Cargando…
Comparative Genomic Hybridization Identifies Virulence Differences in Streptococcus suis
Streptococcus suis is an important zoonotic pathogen. However, identification of virulent S. suis strains is complicated because of the high diversity of the species. Here we evaluated the genetic difference among S. suis strains using comparative genomic hybridization (CGH) and virulence variation...
Autores principales: | , , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2014
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3913679/ https://www.ncbi.nlm.nih.gov/pubmed/24503649 http://dx.doi.org/10.1371/journal.pone.0087866 |
_version_ | 1782302270089592832 |
---|---|
author | Zheng, Han Lan, Ruiting Zheng, Xiao Cui, Zhigang Liu, Zhijie Bai, Xuemei Ji, Shaobo Gottschalk, Marcelo Xu, Jianguo |
author_facet | Zheng, Han Lan, Ruiting Zheng, Xiao Cui, Zhigang Liu, Zhijie Bai, Xuemei Ji, Shaobo Gottschalk, Marcelo Xu, Jianguo |
author_sort | Zheng, Han |
collection | PubMed |
description | Streptococcus suis is an important zoonotic pathogen. However, identification of virulent S. suis strains is complicated because of the high diversity of the species. Here we evaluated the genetic difference among S. suis strains using comparative genomic hybridization (CGH) and virulence variation in vivo and in vitro. We showed that different clades differed in their ability to activate TLR2/6 in vitro and their capacity to induce cytokine production in vivo as well as their resistance to phagocytosis and survival in vivo. Our data showed the S. suis strains tested can be classified into three groups having differing levels of virulence: epidemic and highly virulent strains were clustered into clade Ia (epidemic and highly virulent group, E/HV group), virulent strains were clustered into clade Ib (virulent group, V group), and intermediately or weakly virulent strains were clustered into other clades (intermediately or weakly virulent group, I/WV group). Our study provided further insight into the genomic and virulence variation of S. suis. |
format | Online Article Text |
id | pubmed-3913679 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2014 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-39136792014-02-06 Comparative Genomic Hybridization Identifies Virulence Differences in Streptococcus suis Zheng, Han Lan, Ruiting Zheng, Xiao Cui, Zhigang Liu, Zhijie Bai, Xuemei Ji, Shaobo Gottschalk, Marcelo Xu, Jianguo PLoS One Research Article Streptococcus suis is an important zoonotic pathogen. However, identification of virulent S. suis strains is complicated because of the high diversity of the species. Here we evaluated the genetic difference among S. suis strains using comparative genomic hybridization (CGH) and virulence variation in vivo and in vitro. We showed that different clades differed in their ability to activate TLR2/6 in vitro and their capacity to induce cytokine production in vivo as well as their resistance to phagocytosis and survival in vivo. Our data showed the S. suis strains tested can be classified into three groups having differing levels of virulence: epidemic and highly virulent strains were clustered into clade Ia (epidemic and highly virulent group, E/HV group), virulent strains were clustered into clade Ib (virulent group, V group), and intermediately or weakly virulent strains were clustered into other clades (intermediately or weakly virulent group, I/WV group). Our study provided further insight into the genomic and virulence variation of S. suis. Public Library of Science 2014-02-04 /pmc/articles/PMC3913679/ /pubmed/24503649 http://dx.doi.org/10.1371/journal.pone.0087866 Text en © 2014 Zheng et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited. |
spellingShingle | Research Article Zheng, Han Lan, Ruiting Zheng, Xiao Cui, Zhigang Liu, Zhijie Bai, Xuemei Ji, Shaobo Gottschalk, Marcelo Xu, Jianguo Comparative Genomic Hybridization Identifies Virulence Differences in Streptococcus suis |
title | Comparative Genomic Hybridization Identifies Virulence Differences in Streptococcus suis
|
title_full | Comparative Genomic Hybridization Identifies Virulence Differences in Streptococcus suis
|
title_fullStr | Comparative Genomic Hybridization Identifies Virulence Differences in Streptococcus suis
|
title_full_unstemmed | Comparative Genomic Hybridization Identifies Virulence Differences in Streptococcus suis
|
title_short | Comparative Genomic Hybridization Identifies Virulence Differences in Streptococcus suis
|
title_sort | comparative genomic hybridization identifies virulence differences in streptococcus suis |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3913679/ https://www.ncbi.nlm.nih.gov/pubmed/24503649 http://dx.doi.org/10.1371/journal.pone.0087866 |
work_keys_str_mv | AT zhenghan comparativegenomichybridizationidentifiesvirulencedifferencesinstreptococcussuis AT lanruiting comparativegenomichybridizationidentifiesvirulencedifferencesinstreptococcussuis AT zhengxiao comparativegenomichybridizationidentifiesvirulencedifferencesinstreptococcussuis AT cuizhigang comparativegenomichybridizationidentifiesvirulencedifferencesinstreptococcussuis AT liuzhijie comparativegenomichybridizationidentifiesvirulencedifferencesinstreptococcussuis AT baixuemei comparativegenomichybridizationidentifiesvirulencedifferencesinstreptococcussuis AT jishaobo comparativegenomichybridizationidentifiesvirulencedifferencesinstreptococcussuis AT gottschalkmarcelo comparativegenomichybridizationidentifiesvirulencedifferencesinstreptococcussuis AT xujianguo comparativegenomichybridizationidentifiesvirulencedifferencesinstreptococcussuis |