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Multivariate Analysis Using High Definition Flow Cytometry Reveals Distinct T Cell Repertoires between the Fetal–Maternal Interface and the Peripheral Blood

The human T cell compartment is a complex system and while some information is known on repertoire composition and dynamics in the peripheral blood, little is known about repertoire composition at different anatomical sites. Here, we determine the T cell receptor beta variable (TRBV) repertoire at t...

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Autores principales: Neller, Michelle A., Santner-Nanan, Brigitte, Brennan, Rebekah M., Hsu, Peter, Joung, Steven, Nanan, Ralph, Burrows, Scott R., Miles, John J.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2014
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3913911/
https://www.ncbi.nlm.nih.gov/pubmed/24550917
http://dx.doi.org/10.3389/fimmu.2014.00033
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author Neller, Michelle A.
Santner-Nanan, Brigitte
Brennan, Rebekah M.
Hsu, Peter
Joung, Steven
Nanan, Ralph
Burrows, Scott R.
Miles, John J.
author_facet Neller, Michelle A.
Santner-Nanan, Brigitte
Brennan, Rebekah M.
Hsu, Peter
Joung, Steven
Nanan, Ralph
Burrows, Scott R.
Miles, John J.
author_sort Neller, Michelle A.
collection PubMed
description The human T cell compartment is a complex system and while some information is known on repertoire composition and dynamics in the peripheral blood, little is known about repertoire composition at different anatomical sites. Here, we determine the T cell receptor beta variable (TRBV) repertoire at the decidua and compare it with the peripheral blood during normal pregnancy and pre-eclampsia. We found total T cell subset disparity of up to 58% between sites, including large signature TRBV expansions unique to the fetal–maternal interface. Defining the functional nature and specificity of compartment-specific T cells will be necessary if we are to understand localized immunity, tolerance, and pathogenesis.
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spelling pubmed-39139112014-02-18 Multivariate Analysis Using High Definition Flow Cytometry Reveals Distinct T Cell Repertoires between the Fetal–Maternal Interface and the Peripheral Blood Neller, Michelle A. Santner-Nanan, Brigitte Brennan, Rebekah M. Hsu, Peter Joung, Steven Nanan, Ralph Burrows, Scott R. Miles, John J. Front Immunol Immunology The human T cell compartment is a complex system and while some information is known on repertoire composition and dynamics in the peripheral blood, little is known about repertoire composition at different anatomical sites. Here, we determine the T cell receptor beta variable (TRBV) repertoire at the decidua and compare it with the peripheral blood during normal pregnancy and pre-eclampsia. We found total T cell subset disparity of up to 58% between sites, including large signature TRBV expansions unique to the fetal–maternal interface. Defining the functional nature and specificity of compartment-specific T cells will be necessary if we are to understand localized immunity, tolerance, and pathogenesis. Frontiers Media S.A. 2014-02-05 /pmc/articles/PMC3913911/ /pubmed/24550917 http://dx.doi.org/10.3389/fimmu.2014.00033 Text en Copyright © 2014 Neller, Santner-Nanan, Brennan, Hsu, Joung, Nanan, Burrows and Miles. http://creativecommons.org/licenses/by/3.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Immunology
Neller, Michelle A.
Santner-Nanan, Brigitte
Brennan, Rebekah M.
Hsu, Peter
Joung, Steven
Nanan, Ralph
Burrows, Scott R.
Miles, John J.
Multivariate Analysis Using High Definition Flow Cytometry Reveals Distinct T Cell Repertoires between the Fetal–Maternal Interface and the Peripheral Blood
title Multivariate Analysis Using High Definition Flow Cytometry Reveals Distinct T Cell Repertoires between the Fetal–Maternal Interface and the Peripheral Blood
title_full Multivariate Analysis Using High Definition Flow Cytometry Reveals Distinct T Cell Repertoires between the Fetal–Maternal Interface and the Peripheral Blood
title_fullStr Multivariate Analysis Using High Definition Flow Cytometry Reveals Distinct T Cell Repertoires between the Fetal–Maternal Interface and the Peripheral Blood
title_full_unstemmed Multivariate Analysis Using High Definition Flow Cytometry Reveals Distinct T Cell Repertoires between the Fetal–Maternal Interface and the Peripheral Blood
title_short Multivariate Analysis Using High Definition Flow Cytometry Reveals Distinct T Cell Repertoires between the Fetal–Maternal Interface and the Peripheral Blood
title_sort multivariate analysis using high definition flow cytometry reveals distinct t cell repertoires between the fetal–maternal interface and the peripheral blood
topic Immunology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3913911/
https://www.ncbi.nlm.nih.gov/pubmed/24550917
http://dx.doi.org/10.3389/fimmu.2014.00033
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