Evaluation of In Vitro Antimalarial Activity of Different Extracts of Artemisia aucheri Boiss. and A. armeniaca Lam. and Fractions of the Most Potent Extracts

Ten extracts with different polarity from two Iranian Artemisia species, A. armeniaca Lam. and A. aucheri Boiss, were screened for their antimalarial properties by in vitro   β-hematin formation assay. Dichloromethane (DCM) extracts of both plants showed significant antimalarial activities with IC(5...

Descripción completa

Detalles Bibliográficos
Autores principales: Mojarrab, Mahdi, Shiravand, Ali, Delazar, Abbas, Heshmati Afshar, Fariba
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Hindawi Publishing Corporation 2014
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3914376/
https://www.ncbi.nlm.nih.gov/pubmed/24558335
http://dx.doi.org/10.1155/2014/825370
_version_ 1782302396454535168
author Mojarrab, Mahdi
Shiravand, Ali
Delazar, Abbas
Heshmati Afshar, Fariba
author_facet Mojarrab, Mahdi
Shiravand, Ali
Delazar, Abbas
Heshmati Afshar, Fariba
author_sort Mojarrab, Mahdi
collection PubMed
description Ten extracts with different polarity from two Iranian Artemisia species, A. armeniaca Lam. and A. aucheri Boiss, were screened for their antimalarial properties by in vitro   β-hematin formation assay. Dichloromethane (DCM) extracts of both plants showed significant antimalarial activities with IC(50) values of 1.36 ± 0.01 and 1.83 ± 0.03 mg/mL and IC(90) values of 2.12 ± 0.04 and 2.62 ± 0.09 mg/mL for A. armeniaca and A. aucheri, respectively. Bioactivity-guided fractionation of DCM extracts of both plants by vacuum liquid chromatography (VLC) over silica gel with solvent mixtures of increasing polarities afforded seven fractions. Two fractions from DCM extract of A. armeniaca and four fractions from DCM extract of A. aucheri showed potent antimalarial activity with reducing IC(50) and IC(90) values compared to extracts. The most potent fraction belonged to DCM extract of A. armeniaca with IC(50) and IC(90) values of 0.47 ± 0.006 and 0.71 ± 0.006 mg/mL, respectively.
format Online
Article
Text
id pubmed-3914376
institution National Center for Biotechnology Information
language English
publishDate 2014
publisher Hindawi Publishing Corporation
record_format MEDLINE/PubMed
spelling pubmed-39143762014-02-20 Evaluation of In Vitro Antimalarial Activity of Different Extracts of Artemisia aucheri Boiss. and A. armeniaca Lam. and Fractions of the Most Potent Extracts Mojarrab, Mahdi Shiravand, Ali Delazar, Abbas Heshmati Afshar, Fariba ScientificWorldJournal Research Article Ten extracts with different polarity from two Iranian Artemisia species, A. armeniaca Lam. and A. aucheri Boiss, were screened for their antimalarial properties by in vitro   β-hematin formation assay. Dichloromethane (DCM) extracts of both plants showed significant antimalarial activities with IC(50) values of 1.36 ± 0.01 and 1.83 ± 0.03 mg/mL and IC(90) values of 2.12 ± 0.04 and 2.62 ± 0.09 mg/mL for A. armeniaca and A. aucheri, respectively. Bioactivity-guided fractionation of DCM extracts of both plants by vacuum liquid chromatography (VLC) over silica gel with solvent mixtures of increasing polarities afforded seven fractions. Two fractions from DCM extract of A. armeniaca and four fractions from DCM extract of A. aucheri showed potent antimalarial activity with reducing IC(50) and IC(90) values compared to extracts. The most potent fraction belonged to DCM extract of A. armeniaca with IC(50) and IC(90) values of 0.47 ± 0.006 and 0.71 ± 0.006 mg/mL, respectively. Hindawi Publishing Corporation 2014-01-14 /pmc/articles/PMC3914376/ /pubmed/24558335 http://dx.doi.org/10.1155/2014/825370 Text en Copyright © 2014 Mahdi Mojarrab et al. https://creativecommons.org/licenses/by/3.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Mojarrab, Mahdi
Shiravand, Ali
Delazar, Abbas
Heshmati Afshar, Fariba
Evaluation of In Vitro Antimalarial Activity of Different Extracts of Artemisia aucheri Boiss. and A. armeniaca Lam. and Fractions of the Most Potent Extracts
title Evaluation of In Vitro Antimalarial Activity of Different Extracts of Artemisia aucheri Boiss. and A. armeniaca Lam. and Fractions of the Most Potent Extracts
title_full Evaluation of In Vitro Antimalarial Activity of Different Extracts of Artemisia aucheri Boiss. and A. armeniaca Lam. and Fractions of the Most Potent Extracts
title_fullStr Evaluation of In Vitro Antimalarial Activity of Different Extracts of Artemisia aucheri Boiss. and A. armeniaca Lam. and Fractions of the Most Potent Extracts
title_full_unstemmed Evaluation of In Vitro Antimalarial Activity of Different Extracts of Artemisia aucheri Boiss. and A. armeniaca Lam. and Fractions of the Most Potent Extracts
title_short Evaluation of In Vitro Antimalarial Activity of Different Extracts of Artemisia aucheri Boiss. and A. armeniaca Lam. and Fractions of the Most Potent Extracts
title_sort evaluation of in vitro antimalarial activity of different extracts of artemisia aucheri boiss. and a. armeniaca lam. and fractions of the most potent extracts
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3914376/
https://www.ncbi.nlm.nih.gov/pubmed/24558335
http://dx.doi.org/10.1155/2014/825370
work_keys_str_mv AT mojarrabmahdi evaluationofinvitroantimalarialactivityofdifferentextractsofartemisiaaucheriboissandaarmeniacalamandfractionsofthemostpotentextracts
AT shiravandali evaluationofinvitroantimalarialactivityofdifferentextractsofartemisiaaucheriboissandaarmeniacalamandfractionsofthemostpotentextracts
AT delazarabbas evaluationofinvitroantimalarialactivityofdifferentextractsofartemisiaaucheriboissandaarmeniacalamandfractionsofthemostpotentextracts
AT heshmatiafsharfariba evaluationofinvitroantimalarialactivityofdifferentextractsofartemisiaaucheriboissandaarmeniacalamandfractionsofthemostpotentextracts

Ejemplares similares