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Steroid Production and Follicular Development of Neonatal Mouse Ovary during in vitro Culture

BACKGROUND: The aim of this study was to investigate follicular growth and steroid production in neonatal mouse ovary during in vitro culture. MATERIALS AND METHODS: In this experimental study, 7-day-old mouse whole ovaries were cultured in α-MEM (medium supplemented with 100 mIU/ml recombinant foll...

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Autores principales: Abdi, Shabnam, Salehnia, Mojdeh, Hosseinkhani, Saman
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Royan Institute 2013
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3914497/
https://www.ncbi.nlm.nih.gov/pubmed/24520484
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author Abdi, Shabnam
Salehnia, Mojdeh
Hosseinkhani, Saman
author_facet Abdi, Shabnam
Salehnia, Mojdeh
Hosseinkhani, Saman
author_sort Abdi, Shabnam
collection PubMed
description BACKGROUND: The aim of this study was to investigate follicular growth and steroid production in neonatal mouse ovary during in vitro culture. MATERIALS AND METHODS: In this experimental study, 7-day-old mouse whole ovaries were cultured in α-MEM (medium supplemented with 100 mIU/ml recombinant follicle stimulating hormone, 1% insulin, transferrin and selenium (ITS), 5% fetal bovine serum (FBS), 100 IU/ml penicillin and 50 μg/ml streptomycin for 7 days. The size of whole ovary was determined as mean area during culture. The survival rates of isolated preantral follicles after culture were assessed using trypan blue staining after being mechanically isolated. Histological evaluation of whole ovary was done by hematoxylin and eosin staining. 17-β estradiol, progesterone and dehydroepiandrosterone concentrations in the medium were measured during culture. RESULTS: The mean area of ovary increased after culture (1.47 vs. 0.21 mm(2)). The survival rate of isolated follicles in ovary after culture was 99.2%. There was a significant decline in the percentage of primordial follicles after seven days of culture (91.8 ± 0.2% vs. 65.1 ± 1.1%), whereas the rate of preantral follicles increased significantly (4.6 ± 0.4% vs. 29.2 ± 0.5%). The levels of estradiol, progesterone and dehydroepiandrosterone also increased significantly after culture (p<0.001). CONCLUSION: These results show that the growth and development of primordial follicles in contrast with hormonal production decreased during in vitro culture of neonatal mouse ovaries.
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spelling pubmed-39144972014-02-11 Steroid Production and Follicular Development of Neonatal Mouse Ovary during in vitro Culture Abdi, Shabnam Salehnia, Mojdeh Hosseinkhani, Saman Int J Fertil Steril Research Article BACKGROUND: The aim of this study was to investigate follicular growth and steroid production in neonatal mouse ovary during in vitro culture. MATERIALS AND METHODS: In this experimental study, 7-day-old mouse whole ovaries were cultured in α-MEM (medium supplemented with 100 mIU/ml recombinant follicle stimulating hormone, 1% insulin, transferrin and selenium (ITS), 5% fetal bovine serum (FBS), 100 IU/ml penicillin and 50 μg/ml streptomycin for 7 days. The size of whole ovary was determined as mean area during culture. The survival rates of isolated preantral follicles after culture were assessed using trypan blue staining after being mechanically isolated. Histological evaluation of whole ovary was done by hematoxylin and eosin staining. 17-β estradiol, progesterone and dehydroepiandrosterone concentrations in the medium were measured during culture. RESULTS: The mean area of ovary increased after culture (1.47 vs. 0.21 mm(2)). The survival rate of isolated follicles in ovary after culture was 99.2%. There was a significant decline in the percentage of primordial follicles after seven days of culture (91.8 ± 0.2% vs. 65.1 ± 1.1%), whereas the rate of preantral follicles increased significantly (4.6 ± 0.4% vs. 29.2 ± 0.5%). The levels of estradiol, progesterone and dehydroepiandrosterone also increased significantly after culture (p<0.001). CONCLUSION: These results show that the growth and development of primordial follicles in contrast with hormonal production decreased during in vitro culture of neonatal mouse ovaries. Royan Institute 2013 2013-09-18 /pmc/articles/PMC3914497/ /pubmed/24520484 Text en Any use, distribution, reproduction or abstract of this publication in any medium, with the exception of commercial purposes, is permitted provided the original work is properly cited http://creativecommons.org/licenses/by/2.5/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Abdi, Shabnam
Salehnia, Mojdeh
Hosseinkhani, Saman
Steroid Production and Follicular Development of Neonatal Mouse Ovary during in vitro Culture
title Steroid Production and Follicular Development of Neonatal Mouse Ovary during in vitro Culture
title_full Steroid Production and Follicular Development of Neonatal Mouse Ovary during in vitro Culture
title_fullStr Steroid Production and Follicular Development of Neonatal Mouse Ovary during in vitro Culture
title_full_unstemmed Steroid Production and Follicular Development of Neonatal Mouse Ovary during in vitro Culture
title_short Steroid Production and Follicular Development of Neonatal Mouse Ovary during in vitro Culture
title_sort steroid production and follicular development of neonatal mouse ovary during in vitro culture
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3914497/
https://www.ncbi.nlm.nih.gov/pubmed/24520484
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