Cargando…
Administration of a Probiotic Can Change Drug Pharmacokinetics: Effect of E. coli Nissle 1917 on Amidarone Absorption in Rats
The growing interest in the composition and effects of microbiota raised the question how drug pharmacokinetics could be influenced by concomitant application of probiotics. The aim of this study was to find whether probiotic E. coli strain Nissle 1917 (EcN) influences the pharmacokinetics of concom...
Autores principales: | , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2014
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3914806/ https://www.ncbi.nlm.nih.gov/pubmed/24505278 http://dx.doi.org/10.1371/journal.pone.0087150 |
_version_ | 1782302468137287680 |
---|---|
author | Matuskova, Zuzana Anzenbacherova, Eva Vecera, Rostislav Tlaskalova-Hogenova, Helena Kolar, Milan Anzenbacher, Pavel |
author_facet | Matuskova, Zuzana Anzenbacherova, Eva Vecera, Rostislav Tlaskalova-Hogenova, Helena Kolar, Milan Anzenbacher, Pavel |
author_sort | Matuskova, Zuzana |
collection | PubMed |
description | The growing interest in the composition and effects of microbiota raised the question how drug pharmacokinetics could be influenced by concomitant application of probiotics. The aim of this study was to find whether probiotic E. coli strain Nissle 1917 (EcN) influences the pharmacokinetics of concomitantly taken antiarrhythmic drug amiodarone (AMI). Live bacterial suspension of probiotic EcN (or non-probiotic E. coli strain ATCC 25922) was applied orally to male Wistar rats for seven days, while a control group of rats was treated with a saline solution. On the eighth day, the amiodarone hydrochloride was administered as one single oral dose (50 mg/kg) to all rats (N = 60). After 0, 1, 2, 3, 4, 5.5, 7, 9, 14, 22, and 30 hours, blood samples were taken from the rat abdominal aorta. The plasma level of AMI and its metabolite N-desethylamiodarone (DEA) was determined using the HPLC with UV detection. Administration of EcN led to a 43% increase of AMI AUC(0-30) in comparison with control samples. However, this effect was not observed if EcN was replaced by a reference non-probiotic E. coli strain. Thus, EcN administration was most probably responsible for better drug absorption from the gastrointestinal tract. Plasma levels of DEA were also increased in plasma samples from animals treated with EcN. This change was again not found in the experiment with the reference non-probiotic strain. Higher DEA levels in samples from EcN-treated rats may be explained either by better absorption of AMI and/or by an increased activity of CYP2C forms, known to participate in metabolism of this drug, after EcN administration. In this paper, it is documented that concomitantly taken probiotic EcN may modulate pharmacokinetics of a drug; in this case, it led to an increased bioavailability of AMI. |
format | Online Article Text |
id | pubmed-3914806 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2014 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-39148062014-02-06 Administration of a Probiotic Can Change Drug Pharmacokinetics: Effect of E. coli Nissle 1917 on Amidarone Absorption in Rats Matuskova, Zuzana Anzenbacherova, Eva Vecera, Rostislav Tlaskalova-Hogenova, Helena Kolar, Milan Anzenbacher, Pavel PLoS One Research Article The growing interest in the composition and effects of microbiota raised the question how drug pharmacokinetics could be influenced by concomitant application of probiotics. The aim of this study was to find whether probiotic E. coli strain Nissle 1917 (EcN) influences the pharmacokinetics of concomitantly taken antiarrhythmic drug amiodarone (AMI). Live bacterial suspension of probiotic EcN (or non-probiotic E. coli strain ATCC 25922) was applied orally to male Wistar rats for seven days, while a control group of rats was treated with a saline solution. On the eighth day, the amiodarone hydrochloride was administered as one single oral dose (50 mg/kg) to all rats (N = 60). After 0, 1, 2, 3, 4, 5.5, 7, 9, 14, 22, and 30 hours, blood samples were taken from the rat abdominal aorta. The plasma level of AMI and its metabolite N-desethylamiodarone (DEA) was determined using the HPLC with UV detection. Administration of EcN led to a 43% increase of AMI AUC(0-30) in comparison with control samples. However, this effect was not observed if EcN was replaced by a reference non-probiotic E. coli strain. Thus, EcN administration was most probably responsible for better drug absorption from the gastrointestinal tract. Plasma levels of DEA were also increased in plasma samples from animals treated with EcN. This change was again not found in the experiment with the reference non-probiotic strain. Higher DEA levels in samples from EcN-treated rats may be explained either by better absorption of AMI and/or by an increased activity of CYP2C forms, known to participate in metabolism of this drug, after EcN administration. In this paper, it is documented that concomitantly taken probiotic EcN may modulate pharmacokinetics of a drug; in this case, it led to an increased bioavailability of AMI. Public Library of Science 2014-02-05 /pmc/articles/PMC3914806/ /pubmed/24505278 http://dx.doi.org/10.1371/journal.pone.0087150 Text en © 2014 Matuskova et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited. |
spellingShingle | Research Article Matuskova, Zuzana Anzenbacherova, Eva Vecera, Rostislav Tlaskalova-Hogenova, Helena Kolar, Milan Anzenbacher, Pavel Administration of a Probiotic Can Change Drug Pharmacokinetics: Effect of E. coli Nissle 1917 on Amidarone Absorption in Rats |
title | Administration of a Probiotic Can Change Drug Pharmacokinetics: Effect of E. coli Nissle 1917 on Amidarone Absorption in Rats |
title_full | Administration of a Probiotic Can Change Drug Pharmacokinetics: Effect of E. coli Nissle 1917 on Amidarone Absorption in Rats |
title_fullStr | Administration of a Probiotic Can Change Drug Pharmacokinetics: Effect of E. coli Nissle 1917 on Amidarone Absorption in Rats |
title_full_unstemmed | Administration of a Probiotic Can Change Drug Pharmacokinetics: Effect of E. coli Nissle 1917 on Amidarone Absorption in Rats |
title_short | Administration of a Probiotic Can Change Drug Pharmacokinetics: Effect of E. coli Nissle 1917 on Amidarone Absorption in Rats |
title_sort | administration of a probiotic can change drug pharmacokinetics: effect of e. coli nissle 1917 on amidarone absorption in rats |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3914806/ https://www.ncbi.nlm.nih.gov/pubmed/24505278 http://dx.doi.org/10.1371/journal.pone.0087150 |
work_keys_str_mv | AT matuskovazuzana administrationofaprobioticcanchangedrugpharmacokineticseffectofecolinissle1917onamidaroneabsorptioninrats AT anzenbacherovaeva administrationofaprobioticcanchangedrugpharmacokineticseffectofecolinissle1917onamidaroneabsorptioninrats AT vecerarostislav administrationofaprobioticcanchangedrugpharmacokineticseffectofecolinissle1917onamidaroneabsorptioninrats AT tlaskalovahogenovahelena administrationofaprobioticcanchangedrugpharmacokineticseffectofecolinissle1917onamidaroneabsorptioninrats AT kolarmilan administrationofaprobioticcanchangedrugpharmacokineticseffectofecolinissle1917onamidaroneabsorptioninrats AT anzenbacherpavel administrationofaprobioticcanchangedrugpharmacokineticseffectofecolinissle1917onamidaroneabsorptioninrats |