Cargando…

HTLV-1 Specific CD8+ T Cell Function Augmented by Blockade of 2B4/CD48 Interaction in HTLV-1 Infection

CD8+ T cell response is important in the response to viral infections; this response though is regulated by inhibitory receptors. Expression of inhibitory receptors has been positively correlated with CD8+ T cell exhaustion; the consequent effect of simultaneous blockade of these inhibitory receptor...

Descripción completa

Detalles Bibliográficos
Autores principales: Ezinne, Chibueze Chioma, Yoshimitsu, Makoto, White, Yohann, Arima, Naomichi
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2014
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3914814/
https://www.ncbi.nlm.nih.gov/pubmed/24505299
http://dx.doi.org/10.1371/journal.pone.0087631
_version_ 1782302469973344256
author Ezinne, Chibueze Chioma
Yoshimitsu, Makoto
White, Yohann
Arima, Naomichi
author_facet Ezinne, Chibueze Chioma
Yoshimitsu, Makoto
White, Yohann
Arima, Naomichi
author_sort Ezinne, Chibueze Chioma
collection PubMed
description CD8+ T cell response is important in the response to viral infections; this response though is regulated by inhibitory receptors. Expression of inhibitory receptors has been positively correlated with CD8+ T cell exhaustion; the consequent effect of simultaneous blockade of these inhibitory receptors on CD8+ T cell response in viral infections have been studied, however, the role of individual blockade of receptor-ligand pair is unclear. 2B4/CD48 interaction is involved in CD8+T cell regulation, its signal transducer SAP (signaling lymphocyte activation molecule (SLAM)-associated protein) is required for stimulatory function of 2B4/CD244 on lymphocytes hence, we analyzed 2B4/CD244 (natural killer cell receptor) and SAP (signaling lymphocyte activation molecule(SLAM)-associated protein) on total CD8+ and HTLV-1 specific CD8+T cells in HTLV-1 infection and the effect of blockade of interaction with ligand CD48 on HTLV-1 specific CD8+ T cell function. We observed a high expression of 2B4/CD244 on CD8+ T cells relative to uninfected and further upregulation on HTLV-1 specific CD8+ T cells. 2B4+ CD8+ T cells exhibited more of an effector and terminally differentiated memory phenotype. Blockade of 2B4/CD48 interaction resulted in improvement in function via perforin expression and degranulation as measured by CD107a surface mobilization on HTLV-1 specific CD8+ T cells. In the light of these findings, we thus propose an inhibitory role for 2B4/CD48 interaction on CD8+T cell function.
format Online
Article
Text
id pubmed-3914814
institution National Center for Biotechnology Information
language English
publishDate 2014
publisher Public Library of Science
record_format MEDLINE/PubMed
spelling pubmed-39148142014-02-06 HTLV-1 Specific CD8+ T Cell Function Augmented by Blockade of 2B4/CD48 Interaction in HTLV-1 Infection Ezinne, Chibueze Chioma Yoshimitsu, Makoto White, Yohann Arima, Naomichi PLoS One Research Article CD8+ T cell response is important in the response to viral infections; this response though is regulated by inhibitory receptors. Expression of inhibitory receptors has been positively correlated with CD8+ T cell exhaustion; the consequent effect of simultaneous blockade of these inhibitory receptors on CD8+ T cell response in viral infections have been studied, however, the role of individual blockade of receptor-ligand pair is unclear. 2B4/CD48 interaction is involved in CD8+T cell regulation, its signal transducer SAP (signaling lymphocyte activation molecule (SLAM)-associated protein) is required for stimulatory function of 2B4/CD244 on lymphocytes hence, we analyzed 2B4/CD244 (natural killer cell receptor) and SAP (signaling lymphocyte activation molecule(SLAM)-associated protein) on total CD8+ and HTLV-1 specific CD8+T cells in HTLV-1 infection and the effect of blockade of interaction with ligand CD48 on HTLV-1 specific CD8+ T cell function. We observed a high expression of 2B4/CD244 on CD8+ T cells relative to uninfected and further upregulation on HTLV-1 specific CD8+ T cells. 2B4+ CD8+ T cells exhibited more of an effector and terminally differentiated memory phenotype. Blockade of 2B4/CD48 interaction resulted in improvement in function via perforin expression and degranulation as measured by CD107a surface mobilization on HTLV-1 specific CD8+ T cells. In the light of these findings, we thus propose an inhibitory role for 2B4/CD48 interaction on CD8+T cell function. Public Library of Science 2014-02-05 /pmc/articles/PMC3914814/ /pubmed/24505299 http://dx.doi.org/10.1371/journal.pone.0087631 Text en © 2014 Ezinne et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
spellingShingle Research Article
Ezinne, Chibueze Chioma
Yoshimitsu, Makoto
White, Yohann
Arima, Naomichi
HTLV-1 Specific CD8+ T Cell Function Augmented by Blockade of 2B4/CD48 Interaction in HTLV-1 Infection
title HTLV-1 Specific CD8+ T Cell Function Augmented by Blockade of 2B4/CD48 Interaction in HTLV-1 Infection
title_full HTLV-1 Specific CD8+ T Cell Function Augmented by Blockade of 2B4/CD48 Interaction in HTLV-1 Infection
title_fullStr HTLV-1 Specific CD8+ T Cell Function Augmented by Blockade of 2B4/CD48 Interaction in HTLV-1 Infection
title_full_unstemmed HTLV-1 Specific CD8+ T Cell Function Augmented by Blockade of 2B4/CD48 Interaction in HTLV-1 Infection
title_short HTLV-1 Specific CD8+ T Cell Function Augmented by Blockade of 2B4/CD48 Interaction in HTLV-1 Infection
title_sort htlv-1 specific cd8+ t cell function augmented by blockade of 2b4/cd48 interaction in htlv-1 infection
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3914814/
https://www.ncbi.nlm.nih.gov/pubmed/24505299
http://dx.doi.org/10.1371/journal.pone.0087631
work_keys_str_mv AT ezinnechibuezechioma htlv1specificcd8tcellfunctionaugmentedbyblockadeof2b4cd48interactioninhtlv1infection
AT yoshimitsumakoto htlv1specificcd8tcellfunctionaugmentedbyblockadeof2b4cd48interactioninhtlv1infection
AT whiteyohann htlv1specificcd8tcellfunctionaugmentedbyblockadeof2b4cd48interactioninhtlv1infection
AT arimanaomichi htlv1specificcd8tcellfunctionaugmentedbyblockadeof2b4cd48interactioninhtlv1infection