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Diversity of Conotoxin Gene Superfamilies in the Venomous Snail, Conus victoriae
Animal venoms represent a vast library of bioactive peptides and proteins with proven potential, not only as research tools but also as drug leads and therapeutics. This is illustrated clearly by marine cone snails (genus Conus), whose venoms consist of mixtures of hundreds of peptides (conotoxins)...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2014
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3914837/ https://www.ncbi.nlm.nih.gov/pubmed/24505301 http://dx.doi.org/10.1371/journal.pone.0087648 |
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author | Robinson, Samuel D. Safavi-Hemami, Helena McIntosh, Lachlan D. Purcell, Anthony W. Norton, Raymond S. Papenfuss, Anthony T. |
author_facet | Robinson, Samuel D. Safavi-Hemami, Helena McIntosh, Lachlan D. Purcell, Anthony W. Norton, Raymond S. Papenfuss, Anthony T. |
author_sort | Robinson, Samuel D. |
collection | PubMed |
description | Animal venoms represent a vast library of bioactive peptides and proteins with proven potential, not only as research tools but also as drug leads and therapeutics. This is illustrated clearly by marine cone snails (genus Conus), whose venoms consist of mixtures of hundreds of peptides (conotoxins) with a diverse array of molecular targets, including voltage- and ligand-gated ion channels, G-protein coupled receptors and neurotransmitter transporters. Several conotoxins have found applications as research tools, with some being used or developed as therapeutics. The primary objective of this study was the large-scale discovery of conotoxin sequences from the venom gland of an Australian cone snail species, Conus victoriae. Using cDNA library normalization, high-throughput 454 sequencing, de novo transcriptome assembly and annotation with BLASTX and profile hidden Markov models, we discovered over 100 unique conotoxin sequences from 20 gene superfamilies, the highest diversity of conotoxins so far reported in a single study. Many of the sequences identified are new members of known conotoxin superfamilies, some help to redefine these superfamilies and others represent altogether new classes of conotoxins. In addition, we have demonstrated an efficient combination of methods to mine an animal venom gland and generate a library of sequences encoding bioactive peptides. |
format | Online Article Text |
id | pubmed-3914837 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2014 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-39148372014-02-06 Diversity of Conotoxin Gene Superfamilies in the Venomous Snail, Conus victoriae Robinson, Samuel D. Safavi-Hemami, Helena McIntosh, Lachlan D. Purcell, Anthony W. Norton, Raymond S. Papenfuss, Anthony T. PLoS One Research Article Animal venoms represent a vast library of bioactive peptides and proteins with proven potential, not only as research tools but also as drug leads and therapeutics. This is illustrated clearly by marine cone snails (genus Conus), whose venoms consist of mixtures of hundreds of peptides (conotoxins) with a diverse array of molecular targets, including voltage- and ligand-gated ion channels, G-protein coupled receptors and neurotransmitter transporters. Several conotoxins have found applications as research tools, with some being used or developed as therapeutics. The primary objective of this study was the large-scale discovery of conotoxin sequences from the venom gland of an Australian cone snail species, Conus victoriae. Using cDNA library normalization, high-throughput 454 sequencing, de novo transcriptome assembly and annotation with BLASTX and profile hidden Markov models, we discovered over 100 unique conotoxin sequences from 20 gene superfamilies, the highest diversity of conotoxins so far reported in a single study. Many of the sequences identified are new members of known conotoxin superfamilies, some help to redefine these superfamilies and others represent altogether new classes of conotoxins. In addition, we have demonstrated an efficient combination of methods to mine an animal venom gland and generate a library of sequences encoding bioactive peptides. Public Library of Science 2014-02-05 /pmc/articles/PMC3914837/ /pubmed/24505301 http://dx.doi.org/10.1371/journal.pone.0087648 Text en © 2014 Robinson et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited. |
spellingShingle | Research Article Robinson, Samuel D. Safavi-Hemami, Helena McIntosh, Lachlan D. Purcell, Anthony W. Norton, Raymond S. Papenfuss, Anthony T. Diversity of Conotoxin Gene Superfamilies in the Venomous Snail, Conus victoriae |
title | Diversity of Conotoxin Gene Superfamilies in the Venomous Snail, Conus victoriae
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title_full | Diversity of Conotoxin Gene Superfamilies in the Venomous Snail, Conus victoriae
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title_fullStr | Diversity of Conotoxin Gene Superfamilies in the Venomous Snail, Conus victoriae
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title_full_unstemmed | Diversity of Conotoxin Gene Superfamilies in the Venomous Snail, Conus victoriae
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title_short | Diversity of Conotoxin Gene Superfamilies in the Venomous Snail, Conus victoriae
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title_sort | diversity of conotoxin gene superfamilies in the venomous snail, conus victoriae |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3914837/ https://www.ncbi.nlm.nih.gov/pubmed/24505301 http://dx.doi.org/10.1371/journal.pone.0087648 |
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