Preparation of Phi29 DNA Polymerase Free of Amplifiable DNA Using Ethidium Monoazide, an Ultraviolet-Free Light-Emitting Diode Lamp and Trehalose

We previously reported that multiply-primed rolling circle amplification (MRPCA) using modified random RNA primers can amplify tiny amounts of circular DNA without producing any byproducts. However, contaminating DNA in recombinant Phi29 DNA polymerase adversely affects the outcome of MPRCA, especia...

Descripción completa

Detalles Bibliográficos
Autores principales: Takahashi, Hirokazu, Yamazaki, Hiroyuki, Akanuma, Satoshi, Kanahara, Hiroko, Saito, Toshiyuki, Chimuro, Tomoyuki, Kobayashi, Takayoshi, Ohtani, Toshio, Yamamoto, Kimiko, Sugiyama, Shigeru, Kobori, Toshiro
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2014
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3915000/
https://www.ncbi.nlm.nih.gov/pubmed/24505243
http://dx.doi.org/10.1371/journal.pone.0082624

Ejemplares similares

Ejemplares similares