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A Single-Cell and Feeder-Free Culture System for Monkey Embryonic Stem Cells

Primate pluripotent stem cells (PSCs), including embryonic stem cells (ESCs) and induced pluripotent stem cells (iPSCs), hold great potential for research and application in regenerative medicine and drug discovery. To maximize primate PSC potential, a practical system is required for generating des...

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Autores principales: Ono, Takashi, Suzuki, Yutaka, Kato, Yosuke, Fujita, Risako, Araki, Toshihiro, Yamashita, Tomoko, Kato, Hidemasa, Torii, Ryuzo, Sato, Naoya
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2014
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3915054/
https://www.ncbi.nlm.nih.gov/pubmed/24505480
http://dx.doi.org/10.1371/journal.pone.0088346
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author Ono, Takashi
Suzuki, Yutaka
Kato, Yosuke
Fujita, Risako
Araki, Toshihiro
Yamashita, Tomoko
Kato, Hidemasa
Torii, Ryuzo
Sato, Naoya
author_facet Ono, Takashi
Suzuki, Yutaka
Kato, Yosuke
Fujita, Risako
Araki, Toshihiro
Yamashita, Tomoko
Kato, Hidemasa
Torii, Ryuzo
Sato, Naoya
author_sort Ono, Takashi
collection PubMed
description Primate pluripotent stem cells (PSCs), including embryonic stem cells (ESCs) and induced pluripotent stem cells (iPSCs), hold great potential for research and application in regenerative medicine and drug discovery. To maximize primate PSC potential, a practical system is required for generating desired functional cells and reproducible differentiation techniques. Much progress regarding their culture systems has been reported to date; however, better methods would still be required for their practical use, particularly in industrial and clinical fields. Here we report a new single-cell and feeder-free culture system for primate PSCs, the key feature of which is an originally formulated serum-free medium containing FGF and activin. In this culture system, cynomolgus monkey ESCs can be passaged many times by single-cell dissociation with traditional trypsin treatment and can be propagated with a high proliferation rate as a monolayer without any feeder cells; further, typical PSC properties and genomic stability can be retained. In addition, it has been demonstrated that monkey ESCs maintained in the culture system can be used for various experiments such as in vitro differentiation and gene manipulation. Thus, compared with the conventional culture system, monkey ESCs grown in the aforementioned culture system can serve as a cell source with the following practical advantages: simple, stable, and easy cell maintenance; gene manipulation; cryopreservation; and desired differentiation. We propose that this culture system can serve as a reliable platform to prepare primate PSCs useful for future research and application.
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spelling pubmed-39150542014-02-06 A Single-Cell and Feeder-Free Culture System for Monkey Embryonic Stem Cells Ono, Takashi Suzuki, Yutaka Kato, Yosuke Fujita, Risako Araki, Toshihiro Yamashita, Tomoko Kato, Hidemasa Torii, Ryuzo Sato, Naoya PLoS One Research Article Primate pluripotent stem cells (PSCs), including embryonic stem cells (ESCs) and induced pluripotent stem cells (iPSCs), hold great potential for research and application in regenerative medicine and drug discovery. To maximize primate PSC potential, a practical system is required for generating desired functional cells and reproducible differentiation techniques. Much progress regarding their culture systems has been reported to date; however, better methods would still be required for their practical use, particularly in industrial and clinical fields. Here we report a new single-cell and feeder-free culture system for primate PSCs, the key feature of which is an originally formulated serum-free medium containing FGF and activin. In this culture system, cynomolgus monkey ESCs can be passaged many times by single-cell dissociation with traditional trypsin treatment and can be propagated with a high proliferation rate as a monolayer without any feeder cells; further, typical PSC properties and genomic stability can be retained. In addition, it has been demonstrated that monkey ESCs maintained in the culture system can be used for various experiments such as in vitro differentiation and gene manipulation. Thus, compared with the conventional culture system, monkey ESCs grown in the aforementioned culture system can serve as a cell source with the following practical advantages: simple, stable, and easy cell maintenance; gene manipulation; cryopreservation; and desired differentiation. We propose that this culture system can serve as a reliable platform to prepare primate PSCs useful for future research and application. Public Library of Science 2014-02-05 /pmc/articles/PMC3915054/ /pubmed/24505480 http://dx.doi.org/10.1371/journal.pone.0088346 Text en © 2014 Ono et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
spellingShingle Research Article
Ono, Takashi
Suzuki, Yutaka
Kato, Yosuke
Fujita, Risako
Araki, Toshihiro
Yamashita, Tomoko
Kato, Hidemasa
Torii, Ryuzo
Sato, Naoya
A Single-Cell and Feeder-Free Culture System for Monkey Embryonic Stem Cells
title A Single-Cell and Feeder-Free Culture System for Monkey Embryonic Stem Cells
title_full A Single-Cell and Feeder-Free Culture System for Monkey Embryonic Stem Cells
title_fullStr A Single-Cell and Feeder-Free Culture System for Monkey Embryonic Stem Cells
title_full_unstemmed A Single-Cell and Feeder-Free Culture System for Monkey Embryonic Stem Cells
title_short A Single-Cell and Feeder-Free Culture System for Monkey Embryonic Stem Cells
title_sort single-cell and feeder-free culture system for monkey embryonic stem cells
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3915054/
https://www.ncbi.nlm.nih.gov/pubmed/24505480
http://dx.doi.org/10.1371/journal.pone.0088346
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