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Interaction between DNA and Trimethyl-Ammonium Bromides with Different Alkyl Chain Lengths

The interaction between λ—DNA and cationic surfactants with varying alkyl chain lengths was investigated. By dynamic light scattering method, the trimethyl-ammonium bromides-DNA complex formation was shown to be dependent on the length of the surfactant's alkyl chain. For surfactants with suffi...

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Detalles Bibliográficos
Autores principales: Cheng, Chao, Ran, Shi-Yong
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Hindawi Publishing Corporation 2014
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3915499/
https://www.ncbi.nlm.nih.gov/pubmed/24574926
http://dx.doi.org/10.1155/2014/863049
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author Cheng, Chao
Ran, Shi-Yong
author_facet Cheng, Chao
Ran, Shi-Yong
author_sort Cheng, Chao
collection PubMed
description The interaction between λ—DNA and cationic surfactants with varying alkyl chain lengths was investigated. By dynamic light scattering method, the trimethyl-ammonium bromides-DNA complex formation was shown to be dependent on the length of the surfactant's alkyl chain. For surfactants with sufficient long alkyl chain (CTAB, TTAB, DTAB), the compacted particles exist with a size of ~60–110 nm at low surfactant concentrations. In contrast, high concentration of surfactants leads to aggregates with increased sizes. Atomic force microscope scanning also supports the above observation. Zeta potential measurements show that the potential of the particles decreases with the increase of surfactant concentration (CTAB, TTAB, DTAB), which contributes much to the coagulation of the particles. For OTAB, the surfactant with the shortest chain in this study, it cannot fully neutralize the charges of DNA molecules; consequently, the complex is looser than other surfactant-DNA structures.
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spelling pubmed-39154992014-02-26 Interaction between DNA and Trimethyl-Ammonium Bromides with Different Alkyl Chain Lengths Cheng, Chao Ran, Shi-Yong ScientificWorldJournal Research Article The interaction between λ—DNA and cationic surfactants with varying alkyl chain lengths was investigated. By dynamic light scattering method, the trimethyl-ammonium bromides-DNA complex formation was shown to be dependent on the length of the surfactant's alkyl chain. For surfactants with sufficient long alkyl chain (CTAB, TTAB, DTAB), the compacted particles exist with a size of ~60–110 nm at low surfactant concentrations. In contrast, high concentration of surfactants leads to aggregates with increased sizes. Atomic force microscope scanning also supports the above observation. Zeta potential measurements show that the potential of the particles decreases with the increase of surfactant concentration (CTAB, TTAB, DTAB), which contributes much to the coagulation of the particles. For OTAB, the surfactant with the shortest chain in this study, it cannot fully neutralize the charges of DNA molecules; consequently, the complex is looser than other surfactant-DNA structures. Hindawi Publishing Corporation 2014-01-16 /pmc/articles/PMC3915499/ /pubmed/24574926 http://dx.doi.org/10.1155/2014/863049 Text en Copyright © 2014 C. Cheng and S.-Y. Ran. https://creativecommons.org/licenses/by/3.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Cheng, Chao
Ran, Shi-Yong
Interaction between DNA and Trimethyl-Ammonium Bromides with Different Alkyl Chain Lengths
title Interaction between DNA and Trimethyl-Ammonium Bromides with Different Alkyl Chain Lengths
title_full Interaction between DNA and Trimethyl-Ammonium Bromides with Different Alkyl Chain Lengths
title_fullStr Interaction between DNA and Trimethyl-Ammonium Bromides with Different Alkyl Chain Lengths
title_full_unstemmed Interaction between DNA and Trimethyl-Ammonium Bromides with Different Alkyl Chain Lengths
title_short Interaction between DNA and Trimethyl-Ammonium Bromides with Different Alkyl Chain Lengths
title_sort interaction between dna and trimethyl-ammonium bromides with different alkyl chain lengths
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3915499/
https://www.ncbi.nlm.nih.gov/pubmed/24574926
http://dx.doi.org/10.1155/2014/863049
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