Cargando…
Efficacy of gemcitabine and cetuximab combination treatment in head and neck squamous cell carcinoma
Head and neck squamous cell carcinoma (HNSCC) may be curable with surgery, radiation and chemotherapy in its early stages. However, recurrence and metastasis often prevail following primary treatment in advanced stage cases and are associated with significant morbidity and mortality. In this study w...
Autores principales: | , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
D.A. Spandidos
2013
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3916031/ https://www.ncbi.nlm.nih.gov/pubmed/24649271 http://dx.doi.org/10.3892/mco.2013.159 |
_version_ | 1782302656779255808 |
---|---|
author | MASEKI, SHINICHIRO IJICHI, KEI NAKANISHI, HAYAO HASEGAWA, YASUHISA OGAWA, TETSUYA MURAKAMI, SHINGO |
author_facet | MASEKI, SHINICHIRO IJICHI, KEI NAKANISHI, HAYAO HASEGAWA, YASUHISA OGAWA, TETSUYA MURAKAMI, SHINGO |
author_sort | MASEKI, SHINICHIRO |
collection | PubMed |
description | Head and neck squamous cell carcinoma (HNSCC) may be curable with surgery, radiation and chemotherapy in its early stages. However, recurrence and metastasis often prevail following primary treatment in advanced stage cases and are associated with significant morbidity and mortality. In this study we investigated the combination therapy of gemcitabine and cetuximab for HNSCC. The UM-SCC-6 and UM-SCC-23 HNSCC cell lines were analyzed following treatment with gemcitabine and cetuximab. To determine the mechanism of action of this combination treatment, the cell cycle distributions following gemcitabine and/or cetuximab treatment were analyzed by flow cytometry and apoptosis assay. Gemcitabine and cetuximab combination treatment exerted an enhanced cytotoxic effect. The cell cycle analysis demonstrated that cells accumulated in the S phase following gemcitabine treatment and G1 arrest occurred following cetuximab treatment. An increase in sub-G1 phase cells was also observed following treatment with the two drugs. In an apoptosis assay, caspase 3/7 activity was found to be higher when administering a combination of gemcitabine and cetuximab compared to each agent administered alone. Gemcitabine and cetuximab are individually effective against HNSCC and an enhanced growth inhibitory effect may be expected when these agents are used in combination. |
format | Online Article Text |
id | pubmed-3916031 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2013 |
publisher | D.A. Spandidos |
record_format | MEDLINE/PubMed |
spelling | pubmed-39160312014-03-19 Efficacy of gemcitabine and cetuximab combination treatment in head and neck squamous cell carcinoma MASEKI, SHINICHIRO IJICHI, KEI NAKANISHI, HAYAO HASEGAWA, YASUHISA OGAWA, TETSUYA MURAKAMI, SHINGO Mol Clin Oncol Articles Head and neck squamous cell carcinoma (HNSCC) may be curable with surgery, radiation and chemotherapy in its early stages. However, recurrence and metastasis often prevail following primary treatment in advanced stage cases and are associated with significant morbidity and mortality. In this study we investigated the combination therapy of gemcitabine and cetuximab for HNSCC. The UM-SCC-6 and UM-SCC-23 HNSCC cell lines were analyzed following treatment with gemcitabine and cetuximab. To determine the mechanism of action of this combination treatment, the cell cycle distributions following gemcitabine and/or cetuximab treatment were analyzed by flow cytometry and apoptosis assay. Gemcitabine and cetuximab combination treatment exerted an enhanced cytotoxic effect. The cell cycle analysis demonstrated that cells accumulated in the S phase following gemcitabine treatment and G1 arrest occurred following cetuximab treatment. An increase in sub-G1 phase cells was also observed following treatment with the two drugs. In an apoptosis assay, caspase 3/7 activity was found to be higher when administering a combination of gemcitabine and cetuximab compared to each agent administered alone. Gemcitabine and cetuximab are individually effective against HNSCC and an enhanced growth inhibitory effect may be expected when these agents are used in combination. D.A. Spandidos 2013-09 2013-07-24 /pmc/articles/PMC3916031/ /pubmed/24649271 http://dx.doi.org/10.3892/mco.2013.159 Text en Copyright © 2013, Spandidos Publications http://creativecommons.org/licenses/by/3.0 This is an open-access article licensed under a Creative Commons Attribution-NonCommercial 3.0 Unported License. The article may be redistributed, reproduced, and reused for non-commercial purposes, provided the original source is properly cited. |
spellingShingle | Articles MASEKI, SHINICHIRO IJICHI, KEI NAKANISHI, HAYAO HASEGAWA, YASUHISA OGAWA, TETSUYA MURAKAMI, SHINGO Efficacy of gemcitabine and cetuximab combination treatment in head and neck squamous cell carcinoma |
title | Efficacy of gemcitabine and cetuximab combination treatment in head and neck squamous cell carcinoma |
title_full | Efficacy of gemcitabine and cetuximab combination treatment in head and neck squamous cell carcinoma |
title_fullStr | Efficacy of gemcitabine and cetuximab combination treatment in head and neck squamous cell carcinoma |
title_full_unstemmed | Efficacy of gemcitabine and cetuximab combination treatment in head and neck squamous cell carcinoma |
title_short | Efficacy of gemcitabine and cetuximab combination treatment in head and neck squamous cell carcinoma |
title_sort | efficacy of gemcitabine and cetuximab combination treatment in head and neck squamous cell carcinoma |
topic | Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3916031/ https://www.ncbi.nlm.nih.gov/pubmed/24649271 http://dx.doi.org/10.3892/mco.2013.159 |
work_keys_str_mv | AT masekishinichiro efficacyofgemcitabineandcetuximabcombinationtreatmentinheadandnecksquamouscellcarcinoma AT ijichikei efficacyofgemcitabineandcetuximabcombinationtreatmentinheadandnecksquamouscellcarcinoma AT nakanishihayao efficacyofgemcitabineandcetuximabcombinationtreatmentinheadandnecksquamouscellcarcinoma AT hasegawayasuhisa efficacyofgemcitabineandcetuximabcombinationtreatmentinheadandnecksquamouscellcarcinoma AT ogawatetsuya efficacyofgemcitabineandcetuximabcombinationtreatmentinheadandnecksquamouscellcarcinoma AT murakamishingo efficacyofgemcitabineandcetuximabcombinationtreatmentinheadandnecksquamouscellcarcinoma |