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First Detection of Leishmania tropica DNA and Trypanosoma Species in Sergentomyia Sand Flies (Diptera: Psychodidae) from an Outbreak Area of Cutaneous Leishmaniasis in Ghana

BACKGROUND: Leishmania major and an uncharacterized species have been reported from human patients in a cutaneous leishmaniasis (CL) outbreak area in Ghana. Reports from the area indicate the presence of anthropophilic Sergentomyia species that were found with Leishmania DNA. METHODOLOGY/PRINCIPAL F...

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Autores principales: Nzelu, Chukwunonso O., Kato, Hirotomo, Puplampu, Naiki, Desewu, Kwame, Odoom, Shirley, Wilson, Michael D., Sakurai, Tatsuya, Katakura, Ken, Boakye, Daniel A.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2014
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3916256/
https://www.ncbi.nlm.nih.gov/pubmed/24516676
http://dx.doi.org/10.1371/journal.pntd.0002630
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author Nzelu, Chukwunonso O.
Kato, Hirotomo
Puplampu, Naiki
Desewu, Kwame
Odoom, Shirley
Wilson, Michael D.
Sakurai, Tatsuya
Katakura, Ken
Boakye, Daniel A.
author_facet Nzelu, Chukwunonso O.
Kato, Hirotomo
Puplampu, Naiki
Desewu, Kwame
Odoom, Shirley
Wilson, Michael D.
Sakurai, Tatsuya
Katakura, Ken
Boakye, Daniel A.
author_sort Nzelu, Chukwunonso O.
collection PubMed
description BACKGROUND: Leishmania major and an uncharacterized species have been reported from human patients in a cutaneous leishmaniasis (CL) outbreak area in Ghana. Reports from the area indicate the presence of anthropophilic Sergentomyia species that were found with Leishmania DNA. METHODOLOGY/PRINCIPAL FINDINGS: In this study, we analyzed the Leishmania DNA positive sand fly pools by PCR-RFLP and ITS1 gene sequencing. The trypanosome was determined using the SSU rRNA gene sequence. We observed DNA of L. major, L. tropica and Trypanosoma species to be associated with the sand fly infections. This study provides the first detection of L. tropica DNA and Trypanosoma species as well as the confirmation of L. major DNA within Sergentomyia sand flies in Ghana and suggests that S. ingrami and S. hamoni are possible vectors of CL in the study area. CONCLUSIONS/SIGNIFICANCE: The detection of L. tropica DNA in this CL focus is a novel finding in Ghana as well as West Africa. In addition, the unexpected infection of Trypanosoma DNA within S. africana africana indicates that more attention is necessary when identifying parasitic organisms by PCR within sand fly vectors in Ghana and other areas where leishmaniasis is endemic.
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spelling pubmed-39162562014-02-10 First Detection of Leishmania tropica DNA and Trypanosoma Species in Sergentomyia Sand Flies (Diptera: Psychodidae) from an Outbreak Area of Cutaneous Leishmaniasis in Ghana Nzelu, Chukwunonso O. Kato, Hirotomo Puplampu, Naiki Desewu, Kwame Odoom, Shirley Wilson, Michael D. Sakurai, Tatsuya Katakura, Ken Boakye, Daniel A. PLoS Negl Trop Dis Research Article BACKGROUND: Leishmania major and an uncharacterized species have been reported from human patients in a cutaneous leishmaniasis (CL) outbreak area in Ghana. Reports from the area indicate the presence of anthropophilic Sergentomyia species that were found with Leishmania DNA. METHODOLOGY/PRINCIPAL FINDINGS: In this study, we analyzed the Leishmania DNA positive sand fly pools by PCR-RFLP and ITS1 gene sequencing. The trypanosome was determined using the SSU rRNA gene sequence. We observed DNA of L. major, L. tropica and Trypanosoma species to be associated with the sand fly infections. This study provides the first detection of L. tropica DNA and Trypanosoma species as well as the confirmation of L. major DNA within Sergentomyia sand flies in Ghana and suggests that S. ingrami and S. hamoni are possible vectors of CL in the study area. CONCLUSIONS/SIGNIFICANCE: The detection of L. tropica DNA in this CL focus is a novel finding in Ghana as well as West Africa. In addition, the unexpected infection of Trypanosoma DNA within S. africana africana indicates that more attention is necessary when identifying parasitic organisms by PCR within sand fly vectors in Ghana and other areas where leishmaniasis is endemic. Public Library of Science 2014-02-06 /pmc/articles/PMC3916256/ /pubmed/24516676 http://dx.doi.org/10.1371/journal.pntd.0002630 Text en © 2014 Nzelu et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
spellingShingle Research Article
Nzelu, Chukwunonso O.
Kato, Hirotomo
Puplampu, Naiki
Desewu, Kwame
Odoom, Shirley
Wilson, Michael D.
Sakurai, Tatsuya
Katakura, Ken
Boakye, Daniel A.
First Detection of Leishmania tropica DNA and Trypanosoma Species in Sergentomyia Sand Flies (Diptera: Psychodidae) from an Outbreak Area of Cutaneous Leishmaniasis in Ghana
title First Detection of Leishmania tropica DNA and Trypanosoma Species in Sergentomyia Sand Flies (Diptera: Psychodidae) from an Outbreak Area of Cutaneous Leishmaniasis in Ghana
title_full First Detection of Leishmania tropica DNA and Trypanosoma Species in Sergentomyia Sand Flies (Diptera: Psychodidae) from an Outbreak Area of Cutaneous Leishmaniasis in Ghana
title_fullStr First Detection of Leishmania tropica DNA and Trypanosoma Species in Sergentomyia Sand Flies (Diptera: Psychodidae) from an Outbreak Area of Cutaneous Leishmaniasis in Ghana
title_full_unstemmed First Detection of Leishmania tropica DNA and Trypanosoma Species in Sergentomyia Sand Flies (Diptera: Psychodidae) from an Outbreak Area of Cutaneous Leishmaniasis in Ghana
title_short First Detection of Leishmania tropica DNA and Trypanosoma Species in Sergentomyia Sand Flies (Diptera: Psychodidae) from an Outbreak Area of Cutaneous Leishmaniasis in Ghana
title_sort first detection of leishmania tropica dna and trypanosoma species in sergentomyia sand flies (diptera: psychodidae) from an outbreak area of cutaneous leishmaniasis in ghana
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3916256/
https://www.ncbi.nlm.nih.gov/pubmed/24516676
http://dx.doi.org/10.1371/journal.pntd.0002630
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