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Possible Involvement of Opa-Interacting Protein 5 in Adipose Proliferation and Obesity
Obesity is an epidemic matter increasing risk for cardiovascular diseases and metabolic disorders such as type 2 diabetes. We recently examined the association between visceral fat adiposity and gene expression profile of peripheral blood cells in human subjects. In a series of studies, Opa (Neisser...
Autores principales: | , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2014
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3916335/ https://www.ncbi.nlm.nih.gov/pubmed/24516558 http://dx.doi.org/10.1371/journal.pone.0087661 |
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author | Inoue, Kana Maeda, Norikazu Mori, Takuya Sekimoto, Ryohei Tsushima, Yu Matsuda, Keisuke Yamaoka, Masaya Suganami, Takayoshi Nishizawa, Hitoshi Ogawa, Yoshihiro Funahashi, Tohru Shimomura, Iichiro |
author_facet | Inoue, Kana Maeda, Norikazu Mori, Takuya Sekimoto, Ryohei Tsushima, Yu Matsuda, Keisuke Yamaoka, Masaya Suganami, Takayoshi Nishizawa, Hitoshi Ogawa, Yoshihiro Funahashi, Tohru Shimomura, Iichiro |
author_sort | Inoue, Kana |
collection | PubMed |
description | Obesity is an epidemic matter increasing risk for cardiovascular diseases and metabolic disorders such as type 2 diabetes. We recently examined the association between visceral fat adiposity and gene expression profile of peripheral blood cells in human subjects. In a series of studies, Opa (Neisseria gonorrhoeae opacity-associated)-interacting protein 5 (OIP5) was nominated as a molecule of unknown function in adipocytes and thus the present study was performed to investigate the role of OIP5 in obesity. Adenovirus overexpressing Oip5 (Ad-Oip5) was generated and infected to 3T3-L1 cells stably expressing Coxsackie-Adenovirus Receptor (CAR-3T3-L1) and to mouse subcutaneous fat. For a knockdown experiment, siRNA against Oip5 (Oip5-siRNA) was introduced into 3T3-L1 cells. Proliferation of adipose cells was measured by BrdU uptake, EdU-staining, and cell count. Significant increase of Oip5 mRNA level was observed in obese white adipose tissues and such increase was detected in both mature adipocytes fraction and stromal vascular cell fraction. Ad-Oip5-infected CAR-3T3-L1 preadipocytes and adipocytes proliferated rapidly, while a significant reduction of proliferation was observed in Oip5-siRNA-introduced 3T3-L1 preadipocytes. Fat weight and number of adipocytes were significantly increased in Ad-Oip5-administered fat tissues. Oip5 promotes proliferation of pre- and mature-adipocytes and contributes adipose hyperplasia. Increase of Oip5 may associate with development of obesity. |
format | Online Article Text |
id | pubmed-3916335 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2014 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-39163352014-02-10 Possible Involvement of Opa-Interacting Protein 5 in Adipose Proliferation and Obesity Inoue, Kana Maeda, Norikazu Mori, Takuya Sekimoto, Ryohei Tsushima, Yu Matsuda, Keisuke Yamaoka, Masaya Suganami, Takayoshi Nishizawa, Hitoshi Ogawa, Yoshihiro Funahashi, Tohru Shimomura, Iichiro PLoS One Research Article Obesity is an epidemic matter increasing risk for cardiovascular diseases and metabolic disorders such as type 2 diabetes. We recently examined the association between visceral fat adiposity and gene expression profile of peripheral blood cells in human subjects. In a series of studies, Opa (Neisseria gonorrhoeae opacity-associated)-interacting protein 5 (OIP5) was nominated as a molecule of unknown function in adipocytes and thus the present study was performed to investigate the role of OIP5 in obesity. Adenovirus overexpressing Oip5 (Ad-Oip5) was generated and infected to 3T3-L1 cells stably expressing Coxsackie-Adenovirus Receptor (CAR-3T3-L1) and to mouse subcutaneous fat. For a knockdown experiment, siRNA against Oip5 (Oip5-siRNA) was introduced into 3T3-L1 cells. Proliferation of adipose cells was measured by BrdU uptake, EdU-staining, and cell count. Significant increase of Oip5 mRNA level was observed in obese white adipose tissues and such increase was detected in both mature adipocytes fraction and stromal vascular cell fraction. Ad-Oip5-infected CAR-3T3-L1 preadipocytes and adipocytes proliferated rapidly, while a significant reduction of proliferation was observed in Oip5-siRNA-introduced 3T3-L1 preadipocytes. Fat weight and number of adipocytes were significantly increased in Ad-Oip5-administered fat tissues. Oip5 promotes proliferation of pre- and mature-adipocytes and contributes adipose hyperplasia. Increase of Oip5 may associate with development of obesity. Public Library of Science 2014-02-06 /pmc/articles/PMC3916335/ /pubmed/24516558 http://dx.doi.org/10.1371/journal.pone.0087661 Text en © 2014 Inoue et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited. |
spellingShingle | Research Article Inoue, Kana Maeda, Norikazu Mori, Takuya Sekimoto, Ryohei Tsushima, Yu Matsuda, Keisuke Yamaoka, Masaya Suganami, Takayoshi Nishizawa, Hitoshi Ogawa, Yoshihiro Funahashi, Tohru Shimomura, Iichiro Possible Involvement of Opa-Interacting Protein 5 in Adipose Proliferation and Obesity |
title | Possible Involvement of Opa-Interacting Protein 5 in Adipose Proliferation and Obesity |
title_full | Possible Involvement of Opa-Interacting Protein 5 in Adipose Proliferation and Obesity |
title_fullStr | Possible Involvement of Opa-Interacting Protein 5 in Adipose Proliferation and Obesity |
title_full_unstemmed | Possible Involvement of Opa-Interacting Protein 5 in Adipose Proliferation and Obesity |
title_short | Possible Involvement of Opa-Interacting Protein 5 in Adipose Proliferation and Obesity |
title_sort | possible involvement of opa-interacting protein 5 in adipose proliferation and obesity |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3916335/ https://www.ncbi.nlm.nih.gov/pubmed/24516558 http://dx.doi.org/10.1371/journal.pone.0087661 |
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