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A Gene Expression and Pre-mRNA Splicing Signature That Marks the Adenoma-Adenocarcinoma Progression in Colorectal Cancer

It is widely accepted that most colorectal cancers (CRCs) arise from colorectal adenomas (CRAs), but transcriptomic data characterizing the progression from colorectal normal mucosa to adenoma, and then to adenocarcinoma are scarce. These transition steps were investigated using microarrays, both at...

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Autores principales: Pesson, Marine, Volant, Alain, Uguen, Arnaud, Trillet, Kilian, De La Grange, Pierre, Aubry, Marc, Daoulas, Mélanie, Robaszkiewicz, Michel, Le Gac, Gérald, Morel, Alain, Simon, Brigitte, Corcos, Laurent
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2014
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3916340/
https://www.ncbi.nlm.nih.gov/pubmed/24516561
http://dx.doi.org/10.1371/journal.pone.0087761
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author Pesson, Marine
Volant, Alain
Uguen, Arnaud
Trillet, Kilian
De La Grange, Pierre
Aubry, Marc
Daoulas, Mélanie
Robaszkiewicz, Michel
Le Gac, Gérald
Morel, Alain
Simon, Brigitte
Corcos, Laurent
author_facet Pesson, Marine
Volant, Alain
Uguen, Arnaud
Trillet, Kilian
De La Grange, Pierre
Aubry, Marc
Daoulas, Mélanie
Robaszkiewicz, Michel
Le Gac, Gérald
Morel, Alain
Simon, Brigitte
Corcos, Laurent
author_sort Pesson, Marine
collection PubMed
description It is widely accepted that most colorectal cancers (CRCs) arise from colorectal adenomas (CRAs), but transcriptomic data characterizing the progression from colorectal normal mucosa to adenoma, and then to adenocarcinoma are scarce. These transition steps were investigated using microarrays, both at the level of gene expression and alternative pre-mRNA splicing. Many genes and exons were abnormally expressed in CRAs, even more than in CRCs, as compared to normal mucosae. Known biological pathways involved in CRC were altered in CRA, but several new enriched pathways were also recognized, such as the complement and coagulation cascades. We also identified four intersectional transcriptional signatures that could distinguish CRAs from normal mucosae or CRCs, including a signature of 40 genes differentially deregulated in both CRA and CRC samples. A majority of these genes had been described in different cancers, including FBLN1 or INHBA, but only a few in CRC. Several of these changes were also observed at the protein level. In addition, 20% of these genes (i.e. CFH, CRYAB, DPT, FBLN1, ITIH5, NR3C2, SLIT3 and TIMP1) showed altered pre-mRNA splicing in CRAs. As a global variation occurring since the CRA stage, and maintained in CRC, the expression and splicing changes of this 40-gene set may mark the risk of cancer occurrence from analysis of CRA biopsies.
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spelling pubmed-39163402014-02-10 A Gene Expression and Pre-mRNA Splicing Signature That Marks the Adenoma-Adenocarcinoma Progression in Colorectal Cancer Pesson, Marine Volant, Alain Uguen, Arnaud Trillet, Kilian De La Grange, Pierre Aubry, Marc Daoulas, Mélanie Robaszkiewicz, Michel Le Gac, Gérald Morel, Alain Simon, Brigitte Corcos, Laurent PLoS One Research Article It is widely accepted that most colorectal cancers (CRCs) arise from colorectal adenomas (CRAs), but transcriptomic data characterizing the progression from colorectal normal mucosa to adenoma, and then to adenocarcinoma are scarce. These transition steps were investigated using microarrays, both at the level of gene expression and alternative pre-mRNA splicing. Many genes and exons were abnormally expressed in CRAs, even more than in CRCs, as compared to normal mucosae. Known biological pathways involved in CRC were altered in CRA, but several new enriched pathways were also recognized, such as the complement and coagulation cascades. We also identified four intersectional transcriptional signatures that could distinguish CRAs from normal mucosae or CRCs, including a signature of 40 genes differentially deregulated in both CRA and CRC samples. A majority of these genes had been described in different cancers, including FBLN1 or INHBA, but only a few in CRC. Several of these changes were also observed at the protein level. In addition, 20% of these genes (i.e. CFH, CRYAB, DPT, FBLN1, ITIH5, NR3C2, SLIT3 and TIMP1) showed altered pre-mRNA splicing in CRAs. As a global variation occurring since the CRA stage, and maintained in CRC, the expression and splicing changes of this 40-gene set may mark the risk of cancer occurrence from analysis of CRA biopsies. Public Library of Science 2014-02-06 /pmc/articles/PMC3916340/ /pubmed/24516561 http://dx.doi.org/10.1371/journal.pone.0087761 Text en © 2014 Pesson et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
spellingShingle Research Article
Pesson, Marine
Volant, Alain
Uguen, Arnaud
Trillet, Kilian
De La Grange, Pierre
Aubry, Marc
Daoulas, Mélanie
Robaszkiewicz, Michel
Le Gac, Gérald
Morel, Alain
Simon, Brigitte
Corcos, Laurent
A Gene Expression and Pre-mRNA Splicing Signature That Marks the Adenoma-Adenocarcinoma Progression in Colorectal Cancer
title A Gene Expression and Pre-mRNA Splicing Signature That Marks the Adenoma-Adenocarcinoma Progression in Colorectal Cancer
title_full A Gene Expression and Pre-mRNA Splicing Signature That Marks the Adenoma-Adenocarcinoma Progression in Colorectal Cancer
title_fullStr A Gene Expression and Pre-mRNA Splicing Signature That Marks the Adenoma-Adenocarcinoma Progression in Colorectal Cancer
title_full_unstemmed A Gene Expression and Pre-mRNA Splicing Signature That Marks the Adenoma-Adenocarcinoma Progression in Colorectal Cancer
title_short A Gene Expression and Pre-mRNA Splicing Signature That Marks the Adenoma-Adenocarcinoma Progression in Colorectal Cancer
title_sort gene expression and pre-mrna splicing signature that marks the adenoma-adenocarcinoma progression in colorectal cancer
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3916340/
https://www.ncbi.nlm.nih.gov/pubmed/24516561
http://dx.doi.org/10.1371/journal.pone.0087761
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