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The Sulfated Laminarin Triggers a Stress Transcriptome before Priming the SA- and ROS-Dependent Defenses during Grapevine's Induced Resistance against Plasmopara viticola

Grapevine (Vitis vinifera) is susceptible to many pathogens which cause significant losses to viticulture worldwide. Chemical control is available, but agro-ecological concerns have raised interest in alternative methods, especially in triggering plant immunity by elicitor treatments. The β-glucan l...

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Autores principales: Gauthier, Adrien, Trouvelot, Sophie, Kelloniemi, Jani, Frettinger, Patrick, Wendehenne, David, Daire, Xavier, Joubert, Jean-Marie, Ferrarini, Alberto, Delledonne, Massimo, Flors, Victor, Poinssot, Benoit
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2014
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3916396/
https://www.ncbi.nlm.nih.gov/pubmed/24516597
http://dx.doi.org/10.1371/journal.pone.0088145
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author Gauthier, Adrien
Trouvelot, Sophie
Kelloniemi, Jani
Frettinger, Patrick
Wendehenne, David
Daire, Xavier
Joubert, Jean-Marie
Ferrarini, Alberto
Delledonne, Massimo
Flors, Victor
Poinssot, Benoit
author_facet Gauthier, Adrien
Trouvelot, Sophie
Kelloniemi, Jani
Frettinger, Patrick
Wendehenne, David
Daire, Xavier
Joubert, Jean-Marie
Ferrarini, Alberto
Delledonne, Massimo
Flors, Victor
Poinssot, Benoit
author_sort Gauthier, Adrien
collection PubMed
description Grapevine (Vitis vinifera) is susceptible to many pathogens which cause significant losses to viticulture worldwide. Chemical control is available, but agro-ecological concerns have raised interest in alternative methods, especially in triggering plant immunity by elicitor treatments. The β-glucan laminarin (Lam) and its sulfated derivative (PS3) have been previously demonstrated to induce resistance in grapevine against downy mildew (Plasmopara viticola). However, if Lam elicits classical grapevine defenses such as oxidative burst, pathogenesis-related (PR)-proteins and phytoalexin production, PS3 triggered grapevine resistance via a poorly understood priming phenomenon. The aim of this study was to identify the molecular mechanisms of the PS3-induced resistance. For this purpose we studied i) the signaling events and transcriptome reprogramming triggered by PS3 treatment on uninfected grapevine, ii) grapevine immune responses primed by PS3 during P. viticola infection. Our results showed that i) PS3 was unable to elicit reactive oxygen species (ROS) production, cytosolic Ca(2+) concentration variations, mitogen-activated protein kinase (MAPK) activation but triggered a long lasting plasma membrane depolarization in grapevine cells, ii) PS3 and Lam shared a common stress-responsive transcriptome profile that partly overlapped the salicylate- (SA) and jasmonate-(JA)-dependent ones. After P. viticola inoculation, PS3 specifically primed the SA- and ROS-dependent defense pathways leading to grapevine induced resistance against this biotroph. Interestingly pharmacological approaches suggested that the plasma membrane depolarization and the downstream ROS production are key events of the PS3-induced resistance.
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spelling pubmed-39163962014-02-10 The Sulfated Laminarin Triggers a Stress Transcriptome before Priming the SA- and ROS-Dependent Defenses during Grapevine's Induced Resistance against Plasmopara viticola Gauthier, Adrien Trouvelot, Sophie Kelloniemi, Jani Frettinger, Patrick Wendehenne, David Daire, Xavier Joubert, Jean-Marie Ferrarini, Alberto Delledonne, Massimo Flors, Victor Poinssot, Benoit PLoS One Research Article Grapevine (Vitis vinifera) is susceptible to many pathogens which cause significant losses to viticulture worldwide. Chemical control is available, but agro-ecological concerns have raised interest in alternative methods, especially in triggering plant immunity by elicitor treatments. The β-glucan laminarin (Lam) and its sulfated derivative (PS3) have been previously demonstrated to induce resistance in grapevine against downy mildew (Plasmopara viticola). However, if Lam elicits classical grapevine defenses such as oxidative burst, pathogenesis-related (PR)-proteins and phytoalexin production, PS3 triggered grapevine resistance via a poorly understood priming phenomenon. The aim of this study was to identify the molecular mechanisms of the PS3-induced resistance. For this purpose we studied i) the signaling events and transcriptome reprogramming triggered by PS3 treatment on uninfected grapevine, ii) grapevine immune responses primed by PS3 during P. viticola infection. Our results showed that i) PS3 was unable to elicit reactive oxygen species (ROS) production, cytosolic Ca(2+) concentration variations, mitogen-activated protein kinase (MAPK) activation but triggered a long lasting plasma membrane depolarization in grapevine cells, ii) PS3 and Lam shared a common stress-responsive transcriptome profile that partly overlapped the salicylate- (SA) and jasmonate-(JA)-dependent ones. After P. viticola inoculation, PS3 specifically primed the SA- and ROS-dependent defense pathways leading to grapevine induced resistance against this biotroph. Interestingly pharmacological approaches suggested that the plasma membrane depolarization and the downstream ROS production are key events of the PS3-induced resistance. Public Library of Science 2014-02-06 /pmc/articles/PMC3916396/ /pubmed/24516597 http://dx.doi.org/10.1371/journal.pone.0088145 Text en © 2014 Gauthier et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
spellingShingle Research Article
Gauthier, Adrien
Trouvelot, Sophie
Kelloniemi, Jani
Frettinger, Patrick
Wendehenne, David
Daire, Xavier
Joubert, Jean-Marie
Ferrarini, Alberto
Delledonne, Massimo
Flors, Victor
Poinssot, Benoit
The Sulfated Laminarin Triggers a Stress Transcriptome before Priming the SA- and ROS-Dependent Defenses during Grapevine's Induced Resistance against Plasmopara viticola
title The Sulfated Laminarin Triggers a Stress Transcriptome before Priming the SA- and ROS-Dependent Defenses during Grapevine's Induced Resistance against Plasmopara viticola
title_full The Sulfated Laminarin Triggers a Stress Transcriptome before Priming the SA- and ROS-Dependent Defenses during Grapevine's Induced Resistance against Plasmopara viticola
title_fullStr The Sulfated Laminarin Triggers a Stress Transcriptome before Priming the SA- and ROS-Dependent Defenses during Grapevine's Induced Resistance against Plasmopara viticola
title_full_unstemmed The Sulfated Laminarin Triggers a Stress Transcriptome before Priming the SA- and ROS-Dependent Defenses during Grapevine's Induced Resistance against Plasmopara viticola
title_short The Sulfated Laminarin Triggers a Stress Transcriptome before Priming the SA- and ROS-Dependent Defenses during Grapevine's Induced Resistance against Plasmopara viticola
title_sort sulfated laminarin triggers a stress transcriptome before priming the sa- and ros-dependent defenses during grapevine's induced resistance against plasmopara viticola
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3916396/
https://www.ncbi.nlm.nih.gov/pubmed/24516597
http://dx.doi.org/10.1371/journal.pone.0088145
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