2PE-STED Microscopy with a Single Ti: Sapphire Laser for Reduced Illumination

We reported a new effective approach to carry out two-photon excitation stimulated emission depletion (2PE-STED) microscopy using a single Ti:sapphire laser system. With an acoustic-optic Bragg cell, the modulated-CW 2PE STED microscope had the benefits of both CW and pulse approaches: lower input p...

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Detalles Bibliográficos
Autores principales: Li, Qifeng, Wang, Yang, Chen, Da, Wu, Sherry S. H.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2014
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3916425/
https://www.ncbi.nlm.nih.gov/pubmed/24516661
http://dx.doi.org/10.1371/journal.pone.0088464
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author Li, Qifeng
Wang, Yang
Chen, Da
Wu, Sherry S. H.
author_facet Li, Qifeng
Wang, Yang
Chen, Da
Wu, Sherry S. H.
author_sort Li, Qifeng
collection PubMed
description We reported a new effective approach to carry out two-photon excitation stimulated emission depletion (2PE-STED) microscopy using a single Ti:sapphire laser system. With an acoustic-optic Bragg cell, the modulated-CW 2PE STED microscope had the benefits of both CW and pulse approaches: lower input power, simple optical scheme and no complicated synchronization. Additionally, it also took advantages of fluorescence yield increasing. The sub-diffraction-limit resolution was demonstrated using ATTO 425-tagged clathrin-coated vesicles.
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spelling pubmed-39164252014-02-10 2PE-STED Microscopy with a Single Ti: Sapphire Laser for Reduced Illumination Li, Qifeng Wang, Yang Chen, Da Wu, Sherry S. H. PLoS One Research Article We reported a new effective approach to carry out two-photon excitation stimulated emission depletion (2PE-STED) microscopy using a single Ti:sapphire laser system. With an acoustic-optic Bragg cell, the modulated-CW 2PE STED microscope had the benefits of both CW and pulse approaches: lower input power, simple optical scheme and no complicated synchronization. Additionally, it also took advantages of fluorescence yield increasing. The sub-diffraction-limit resolution was demonstrated using ATTO 425-tagged clathrin-coated vesicles. Public Library of Science 2014-02-06 /pmc/articles/PMC3916425/ /pubmed/24516661 http://dx.doi.org/10.1371/journal.pone.0088464 Text en © 2014 Li et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
spellingShingle Research Article
Li, Qifeng
Wang, Yang
Chen, Da
Wu, Sherry S. H.
2PE-STED Microscopy with a Single Ti: Sapphire Laser for Reduced Illumination
title 2PE-STED Microscopy with a Single Ti: Sapphire Laser for Reduced Illumination
title_full 2PE-STED Microscopy with a Single Ti: Sapphire Laser for Reduced Illumination
title_fullStr 2PE-STED Microscopy with a Single Ti: Sapphire Laser for Reduced Illumination
title_full_unstemmed 2PE-STED Microscopy with a Single Ti: Sapphire Laser for Reduced Illumination
title_short 2PE-STED Microscopy with a Single Ti: Sapphire Laser for Reduced Illumination
title_sort 2pe-sted microscopy with a single ti: sapphire laser for reduced illumination
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3916425/
https://www.ncbi.nlm.nih.gov/pubmed/24516661
http://dx.doi.org/10.1371/journal.pone.0088464
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AT wusherrysh 2pestedmicroscopywithasingletisapphirelaserforreducedillumination

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