Chymase Activities and Survival in Endotoxin-Induced Human Chymase Transgenic Mice

We examined the effects of overexpressed human chymase on survival and activity in lipopolysaccharide (LPS)-treated mice. Human chymase transgenic (Tg) and wild-type C57BL/6 (WT) mice were treated with LPS (0.03, 0.1 and 0.3 mg/day; intraperitoneal) for 2 weeks. Treatment with 0.03 mg LPS did not af...

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Detalles Bibliográficos
Autores principales: Rafiq, Kazi, Fan, Yu-Yan, Sherajee, Shamshad J., Takahashi, Yoshimasa, Matsuura, Junji, Hase, Naoki, Mori, Hirohito, Nakano, Daisuke, Kobara, Hideki, Hitomi, Hirofumi, Masaki, Tsutomu, Urata, Hidenori, Nishiyama, Akira
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Ivyspring International Publisher 2014
Materias:
Short Research Communication
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3917108/
https://www.ncbi.nlm.nih.gov/pubmed/24516344
http://dx.doi.org/10.7150/ijms.7382
Descripción
Sumario:We examined the effects of overexpressed human chymase on survival and activity in lipopolysaccharide (LPS)-treated mice. Human chymase transgenic (Tg) and wild-type C57BL/6 (WT) mice were treated with LPS (0.03, 0.1 and 0.3 mg/day; intraperitoneal) for 2 weeks. Treatment with 0.03 mg LPS did not affect survival in either WT or Tg mice. WT mice were not affected by 0.1 mg/day of LPS, whereas 25% of Tg mice died. Survival of mice treated with 0.3 mg/day of LPS was 87.5% and 0% in WT and Tg, respectively. LPS-induced increases in chymase activity in the heart and skin were significantly greater in Tg than WT mice. These data suggest a possible contribution of human chymase activation to LPS-induced mortality.

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