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Escherichia coli swimming is robust against variations in flagellar number
Bacterial chemotaxis is a paradigm for how environmental signals modulate cellular behavior. Although the network underlying this process has been studied extensively, we do not yet have an end-to-end understanding of chemotaxis. Specifically, how the rotational states of a cell’s flagella cooperati...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
eLife Sciences Publications, Ltd
2014
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3917375/ https://www.ncbi.nlm.nih.gov/pubmed/24520165 http://dx.doi.org/10.7554/eLife.01916 |
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author | Mears, Patrick J Koirala, Santosh Rao, Chris V Golding, Ido Chemla, Yann R |
author_facet | Mears, Patrick J Koirala, Santosh Rao, Chris V Golding, Ido Chemla, Yann R |
author_sort | Mears, Patrick J |
collection | PubMed |
description | Bacterial chemotaxis is a paradigm for how environmental signals modulate cellular behavior. Although the network underlying this process has been studied extensively, we do not yet have an end-to-end understanding of chemotaxis. Specifically, how the rotational states of a cell’s flagella cooperatively determine whether the cell ‘runs’ or ‘tumbles’ remains poorly characterized. Here, we measure the swimming behavior of individual E. coli cells while simultaneously detecting the rotational states of each flagellum. We find that a simple mathematical expression relates the cell’s run/tumble bias to the number and average rotational state of its flagella. However, due to inter-flagellar correlations, an ‘effective number’ of flagella—smaller than the actual number—enters into this relation. Data from a chemotaxis mutant and stochastic modeling suggest that fluctuations of the regulator CheY-P are the source of flagellar correlations. A consequence of inter-flagellar correlations is that run/tumble behavior is only weakly dependent on number of flagella. DOI: http://dx.doi.org/10.7554/eLife.01916.001 |
format | Online Article Text |
id | pubmed-3917375 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2014 |
publisher | eLife Sciences Publications, Ltd |
record_format | MEDLINE/PubMed |
spelling | pubmed-39173752014-02-12 Escherichia coli swimming is robust against variations in flagellar number Mears, Patrick J Koirala, Santosh Rao, Chris V Golding, Ido Chemla, Yann R eLife Biophysics and Structural Biology Bacterial chemotaxis is a paradigm for how environmental signals modulate cellular behavior. Although the network underlying this process has been studied extensively, we do not yet have an end-to-end understanding of chemotaxis. Specifically, how the rotational states of a cell’s flagella cooperatively determine whether the cell ‘runs’ or ‘tumbles’ remains poorly characterized. Here, we measure the swimming behavior of individual E. coli cells while simultaneously detecting the rotational states of each flagellum. We find that a simple mathematical expression relates the cell’s run/tumble bias to the number and average rotational state of its flagella. However, due to inter-flagellar correlations, an ‘effective number’ of flagella—smaller than the actual number—enters into this relation. Data from a chemotaxis mutant and stochastic modeling suggest that fluctuations of the regulator CheY-P are the source of flagellar correlations. A consequence of inter-flagellar correlations is that run/tumble behavior is only weakly dependent on number of flagella. DOI: http://dx.doi.org/10.7554/eLife.01916.001 eLife Sciences Publications, Ltd 2014-02-11 /pmc/articles/PMC3917375/ /pubmed/24520165 http://dx.doi.org/10.7554/eLife.01916 Text en Copyright © 2013, Mears et al http://creativecommons.org/licenses/by/3.0/ This article is distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/3.0/) , which permits unrestricted use and redistribution provided that the original author and source are credited. |
spellingShingle | Biophysics and Structural Biology Mears, Patrick J Koirala, Santosh Rao, Chris V Golding, Ido Chemla, Yann R Escherichia coli swimming is robust against variations in flagellar number |
title | Escherichia coli swimming is robust against variations in flagellar number |
title_full | Escherichia coli swimming is robust against variations in flagellar number |
title_fullStr | Escherichia coli swimming is robust against variations in flagellar number |
title_full_unstemmed | Escherichia coli swimming is robust against variations in flagellar number |
title_short | Escherichia coli swimming is robust against variations in flagellar number |
title_sort | escherichia coli swimming is robust against variations in flagellar number |
topic | Biophysics and Structural Biology |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3917375/ https://www.ncbi.nlm.nih.gov/pubmed/24520165 http://dx.doi.org/10.7554/eLife.01916 |
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