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First Molecular Evidence for Puumala Hantavirus in Poland
Puumala virus (PUUV) causes mild to moderate cases of haemorrhagic fever with renal syndrome (HFRS), and is responsible for the majority of hantavirus infections of humans in Fennoscandia, Central and Western Europe. Although there are relatively many PUUV sequences available from different European...
Autores principales: | , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2014
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3917447/ https://www.ncbi.nlm.nih.gov/pubmed/24452006 http://dx.doi.org/10.3390/v6010340 |
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author | Sheikh Ali, Hanan Drewes, Stephan Sadowska, Edyta T. Mikowska, Magdalena Groschup, Martin H. Heckel, Gerald Koteja, Pawel Ulrich, Rainer G. |
author_facet | Sheikh Ali, Hanan Drewes, Stephan Sadowska, Edyta T. Mikowska, Magdalena Groschup, Martin H. Heckel, Gerald Koteja, Pawel Ulrich, Rainer G. |
author_sort | Sheikh Ali, Hanan |
collection | PubMed |
description | Puumala virus (PUUV) causes mild to moderate cases of haemorrhagic fever with renal syndrome (HFRS), and is responsible for the majority of hantavirus infections of humans in Fennoscandia, Central and Western Europe. Although there are relatively many PUUV sequences available from different European countries, little is known about the presence of this virus in Poland. During population studies in 2009 a total of 45 bank voles were trapped at three sites in north-eastern Poland, namely islands on Dejguny and Dobskie Lakes and in a forest near Mikołajki. S and M segment-specific RT-PCR assays detected PUUV RNA in three animals from the Mikołajki site. The obtained partial S and M segment sequences demonstrated the highest similarity to the corresponding segments of a PUUV strain from Latvia. Analysis of chest cavity fluid samples by IgG ELISA using a yeast-expressed PUUV nucleocapsid protein resulted in the detection of two seropositive samples, both being also RT-PCR positive. Interestingly, at the trapping site in Mikołajki PUUV-positive bank voles belong to the Carpathian and Eastern genetic lineages within this species. In conclusion, we herein present the first molecular evidence for PUUV in the rodent reservoir from Poland. |
format | Online Article Text |
id | pubmed-3917447 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2014 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-39174472014-02-07 First Molecular Evidence for Puumala Hantavirus in Poland Sheikh Ali, Hanan Drewes, Stephan Sadowska, Edyta T. Mikowska, Magdalena Groschup, Martin H. Heckel, Gerald Koteja, Pawel Ulrich, Rainer G. Viruses Brief Report Puumala virus (PUUV) causes mild to moderate cases of haemorrhagic fever with renal syndrome (HFRS), and is responsible for the majority of hantavirus infections of humans in Fennoscandia, Central and Western Europe. Although there are relatively many PUUV sequences available from different European countries, little is known about the presence of this virus in Poland. During population studies in 2009 a total of 45 bank voles were trapped at three sites in north-eastern Poland, namely islands on Dejguny and Dobskie Lakes and in a forest near Mikołajki. S and M segment-specific RT-PCR assays detected PUUV RNA in three animals from the Mikołajki site. The obtained partial S and M segment sequences demonstrated the highest similarity to the corresponding segments of a PUUV strain from Latvia. Analysis of chest cavity fluid samples by IgG ELISA using a yeast-expressed PUUV nucleocapsid protein resulted in the detection of two seropositive samples, both being also RT-PCR positive. Interestingly, at the trapping site in Mikołajki PUUV-positive bank voles belong to the Carpathian and Eastern genetic lineages within this species. In conclusion, we herein present the first molecular evidence for PUUV in the rodent reservoir from Poland. MDPI 2014-01-23 /pmc/articles/PMC3917447/ /pubmed/24452006 http://dx.doi.org/10.3390/v6010340 Text en © 2014 by the authors; licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/3.0/). |
spellingShingle | Brief Report Sheikh Ali, Hanan Drewes, Stephan Sadowska, Edyta T. Mikowska, Magdalena Groschup, Martin H. Heckel, Gerald Koteja, Pawel Ulrich, Rainer G. First Molecular Evidence for Puumala Hantavirus in Poland |
title | First Molecular Evidence for Puumala Hantavirus in Poland |
title_full | First Molecular Evidence for Puumala Hantavirus in Poland |
title_fullStr | First Molecular Evidence for Puumala Hantavirus in Poland |
title_full_unstemmed | First Molecular Evidence for Puumala Hantavirus in Poland |
title_short | First Molecular Evidence for Puumala Hantavirus in Poland |
title_sort | first molecular evidence for puumala hantavirus in poland |
topic | Brief Report |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3917447/ https://www.ncbi.nlm.nih.gov/pubmed/24452006 http://dx.doi.org/10.3390/v6010340 |
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