Secretory production of an FAD cofactor-containing cytosolic enzyme (sorbitol–xylitol oxidase from Streptomyces coelicolor) using the twin-arginine translocation (Tat) pathway of Corynebacterium glutamicum

Carbohydrate oxidases are biotechnologically interesting enzymes that require a tightly or covalently bound cofactor for activity. Using the industrial workhorse Corynebacterium glutamicum as the expression host, successful secretion of a normally cytosolic FAD cofactor-containing sorbitol–xylitol o...

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Detalles Bibliográficos
Autores principales: Scheele, Sandra, Oertel, Dan, Bongaerts, Johannes, Evers, Stefan, Hellmuth, Hendrik, Maurer, Karl-Heinz, Bott, Michael, Freudl, Roland
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Blackwell Publishing Ltd 2013
Materias:
Brief Reports
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3917463/
https://www.ncbi.nlm.nih.gov/pubmed/23163932
http://dx.doi.org/10.1111/1751-7915.12005

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