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Alveolar Macrophages Infected with Ames or Sterne Strain of Bacillus anthracis Elicit Differential Molecular Expression Patterns

Alveolar macrophages (AMs) phagocytose Bacillus anthracis following inhalation and induce the production of pro-inflammatory cytokines and chemokines to mediate the activation of innate immunity. Ames, the virulent strain of B. anthracis, contains two plasmids that encode the antiphagocytic poly-γ-d...

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Autores principales: Langel, Felicia D., Chiang, Chih-Yuan, Lane, Douglas, Kenny, Tara, Ojeda, Jenifer F., Zhong, Yang, Che, Jianwei, Zhou, Yingyao, Ribot, Wilson, Kota, Krishna P., Bavari, Sina, Panchal, Rekha G.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2014
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3917846/
https://www.ncbi.nlm.nih.gov/pubmed/24516547
http://dx.doi.org/10.1371/journal.pone.0087201
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author Langel, Felicia D.
Chiang, Chih-Yuan
Lane, Douglas
Kenny, Tara
Ojeda, Jenifer F.
Zhong, Yang
Che, Jianwei
Zhou, Yingyao
Ribot, Wilson
Kota, Krishna P.
Bavari, Sina
Panchal, Rekha G.
author_facet Langel, Felicia D.
Chiang, Chih-Yuan
Lane, Douglas
Kenny, Tara
Ojeda, Jenifer F.
Zhong, Yang
Che, Jianwei
Zhou, Yingyao
Ribot, Wilson
Kota, Krishna P.
Bavari, Sina
Panchal, Rekha G.
author_sort Langel, Felicia D.
collection PubMed
description Alveolar macrophages (AMs) phagocytose Bacillus anthracis following inhalation and induce the production of pro-inflammatory cytokines and chemokines to mediate the activation of innate immunity. Ames, the virulent strain of B. anthracis, contains two plasmids that encode the antiphagocytic poly-γ-d-glutamic acid capsule and the lethal toxin. The attenuated Sterne strain of B. anthracis, which lacks the plasmid encoding capsule, is widely adapted as a vaccine strain. Although differences in the outcome of infection with the two strains may have originated from the presence or absence of an anti-phagocytic capsule, the disease pathogenesis following infection will be manifested via the host responses, which is not well understood. To gain understanding of the host responses at cellular level, a microarray analysis was performed using primary rhesus macaque AMs infected with either Ames or Sterne spores. Notably, 528 human orthologs were identified to be differentially expressed in AMs infected with either strain of the B. anthracis. Meta-analyses revealed genes differentially expressed in response to B. anthracis infection were also induced upon infections with multiple pathogens such as Francisella Novicida or Staphylococcus aureus. This suggests the existence of a common molecular signature in response to pathogen infections. Importantly, the microarray and protein expression data for certain cytokines, chemokines and host factors provide further insights on how cellular processes such as innate immune sensing pathways, anti-apoptosis versus apoptosis may be differentially modulated in response to the virulent or vaccine strain of B. anthracis. The reported differences may account for the marked difference in pathogenicity between these two strains.
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spelling pubmed-39178462014-02-10 Alveolar Macrophages Infected with Ames or Sterne Strain of Bacillus anthracis Elicit Differential Molecular Expression Patterns Langel, Felicia D. Chiang, Chih-Yuan Lane, Douglas Kenny, Tara Ojeda, Jenifer F. Zhong, Yang Che, Jianwei Zhou, Yingyao Ribot, Wilson Kota, Krishna P. Bavari, Sina Panchal, Rekha G. PLoS One Research Article Alveolar macrophages (AMs) phagocytose Bacillus anthracis following inhalation and induce the production of pro-inflammatory cytokines and chemokines to mediate the activation of innate immunity. Ames, the virulent strain of B. anthracis, contains two plasmids that encode the antiphagocytic poly-γ-d-glutamic acid capsule and the lethal toxin. The attenuated Sterne strain of B. anthracis, which lacks the plasmid encoding capsule, is widely adapted as a vaccine strain. Although differences in the outcome of infection with the two strains may have originated from the presence or absence of an anti-phagocytic capsule, the disease pathogenesis following infection will be manifested via the host responses, which is not well understood. To gain understanding of the host responses at cellular level, a microarray analysis was performed using primary rhesus macaque AMs infected with either Ames or Sterne spores. Notably, 528 human orthologs were identified to be differentially expressed in AMs infected with either strain of the B. anthracis. Meta-analyses revealed genes differentially expressed in response to B. anthracis infection were also induced upon infections with multiple pathogens such as Francisella Novicida or Staphylococcus aureus. This suggests the existence of a common molecular signature in response to pathogen infections. Importantly, the microarray and protein expression data for certain cytokines, chemokines and host factors provide further insights on how cellular processes such as innate immune sensing pathways, anti-apoptosis versus apoptosis may be differentially modulated in response to the virulent or vaccine strain of B. anthracis. The reported differences may account for the marked difference in pathogenicity between these two strains. Public Library of Science 2014-02-07 /pmc/articles/PMC3917846/ /pubmed/24516547 http://dx.doi.org/10.1371/journal.pone.0087201 Text en https://creativecommons.org/publicdomain/zero/1.0/ This is an open-access article distributed under the terms of the Creative Commons Public Domain declaration, which stipulates that, once placed in the public domain, this work may be freely reproduced, distributed, transmitted, modified, built upon, or otherwise used by anyone for any lawful purpose.
spellingShingle Research Article
Langel, Felicia D.
Chiang, Chih-Yuan
Lane, Douglas
Kenny, Tara
Ojeda, Jenifer F.
Zhong, Yang
Che, Jianwei
Zhou, Yingyao
Ribot, Wilson
Kota, Krishna P.
Bavari, Sina
Panchal, Rekha G.
Alveolar Macrophages Infected with Ames or Sterne Strain of Bacillus anthracis Elicit Differential Molecular Expression Patterns
title Alveolar Macrophages Infected with Ames or Sterne Strain of Bacillus anthracis Elicit Differential Molecular Expression Patterns
title_full Alveolar Macrophages Infected with Ames or Sterne Strain of Bacillus anthracis Elicit Differential Molecular Expression Patterns
title_fullStr Alveolar Macrophages Infected with Ames or Sterne Strain of Bacillus anthracis Elicit Differential Molecular Expression Patterns
title_full_unstemmed Alveolar Macrophages Infected with Ames or Sterne Strain of Bacillus anthracis Elicit Differential Molecular Expression Patterns
title_short Alveolar Macrophages Infected with Ames or Sterne Strain of Bacillus anthracis Elicit Differential Molecular Expression Patterns
title_sort alveolar macrophages infected with ames or sterne strain of bacillus anthracis elicit differential molecular expression patterns
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3917846/
https://www.ncbi.nlm.nih.gov/pubmed/24516547
http://dx.doi.org/10.1371/journal.pone.0087201
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