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TLR2, TLR4 and CD14 Recognize Venom-Associated Molecular Patterns from Tityus serrulatus to Induce Macrophage-Derived Inflammatory Mediators

Scorpion sting-induced human envenomation provokes an intense inflammatory reaction. However, the mechanisms behind the recognition of scorpion venom and the induction of mediator release in mammalian cells are unknown. We demonstrated that TLR2, TLR4 and CD14 receptors sense Tityus serrulatus venom...

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Autores principales: Zoccal, Karina Furlani, Bitencourt, Claudia da Silva, Paula-Silva, Francisco Wanderley Garcia, Sorgi, Carlos Artério, de Castro Figueiredo Bordon, Karla, Arantes, Eliane Candiani, Faccioli, Lúcia Helena
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2014
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3917877/
https://www.ncbi.nlm.nih.gov/pubmed/24516606
http://dx.doi.org/10.1371/journal.pone.0088174
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author Zoccal, Karina Furlani
Bitencourt, Claudia da Silva
Paula-Silva, Francisco Wanderley Garcia
Sorgi, Carlos Artério
de Castro Figueiredo Bordon, Karla
Arantes, Eliane Candiani
Faccioli, Lúcia Helena
author_facet Zoccal, Karina Furlani
Bitencourt, Claudia da Silva
Paula-Silva, Francisco Wanderley Garcia
Sorgi, Carlos Artério
de Castro Figueiredo Bordon, Karla
Arantes, Eliane Candiani
Faccioli, Lúcia Helena
author_sort Zoccal, Karina Furlani
collection PubMed
description Scorpion sting-induced human envenomation provokes an intense inflammatory reaction. However, the mechanisms behind the recognition of scorpion venom and the induction of mediator release in mammalian cells are unknown. We demonstrated that TLR2, TLR4 and CD14 receptors sense Tityus serrulatus venom (TsV) and its major component, toxin 1 (Ts1), to mediate cytokine and lipid mediator production. Additionally, we demonstrated that TsV induces TLR2- and TLR4/MyD88-dependent NF-κB activation and TLR4-dependent and TLR2/MyD88-independent c-Jun activation. Similar to TsV, Ts1 induces MyD88-dependent NF-κB phosphorylation via TLR2 and TLR4 receptors, while c-Jun activation is dependent on neither TLR2 nor TLR4/MyD88. Therefore, we propose the term venom-associated molecular pattern (VAMP) to refer to molecules that are introduced into the host by stings and are recognized by PRRs, resulting in inflammation.
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spelling pubmed-39178772014-02-10 TLR2, TLR4 and CD14 Recognize Venom-Associated Molecular Patterns from Tityus serrulatus to Induce Macrophage-Derived Inflammatory Mediators Zoccal, Karina Furlani Bitencourt, Claudia da Silva Paula-Silva, Francisco Wanderley Garcia Sorgi, Carlos Artério de Castro Figueiredo Bordon, Karla Arantes, Eliane Candiani Faccioli, Lúcia Helena PLoS One Research Article Scorpion sting-induced human envenomation provokes an intense inflammatory reaction. However, the mechanisms behind the recognition of scorpion venom and the induction of mediator release in mammalian cells are unknown. We demonstrated that TLR2, TLR4 and CD14 receptors sense Tityus serrulatus venom (TsV) and its major component, toxin 1 (Ts1), to mediate cytokine and lipid mediator production. Additionally, we demonstrated that TsV induces TLR2- and TLR4/MyD88-dependent NF-κB activation and TLR4-dependent and TLR2/MyD88-independent c-Jun activation. Similar to TsV, Ts1 induces MyD88-dependent NF-κB phosphorylation via TLR2 and TLR4 receptors, while c-Jun activation is dependent on neither TLR2 nor TLR4/MyD88. Therefore, we propose the term venom-associated molecular pattern (VAMP) to refer to molecules that are introduced into the host by stings and are recognized by PRRs, resulting in inflammation. Public Library of Science 2014-02-07 /pmc/articles/PMC3917877/ /pubmed/24516606 http://dx.doi.org/10.1371/journal.pone.0088174 Text en © 2014 Zoccal et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
spellingShingle Research Article
Zoccal, Karina Furlani
Bitencourt, Claudia da Silva
Paula-Silva, Francisco Wanderley Garcia
Sorgi, Carlos Artério
de Castro Figueiredo Bordon, Karla
Arantes, Eliane Candiani
Faccioli, Lúcia Helena
TLR2, TLR4 and CD14 Recognize Venom-Associated Molecular Patterns from Tityus serrulatus to Induce Macrophage-Derived Inflammatory Mediators
title TLR2, TLR4 and CD14 Recognize Venom-Associated Molecular Patterns from Tityus serrulatus to Induce Macrophage-Derived Inflammatory Mediators
title_full TLR2, TLR4 and CD14 Recognize Venom-Associated Molecular Patterns from Tityus serrulatus to Induce Macrophage-Derived Inflammatory Mediators
title_fullStr TLR2, TLR4 and CD14 Recognize Venom-Associated Molecular Patterns from Tityus serrulatus to Induce Macrophage-Derived Inflammatory Mediators
title_full_unstemmed TLR2, TLR4 and CD14 Recognize Venom-Associated Molecular Patterns from Tityus serrulatus to Induce Macrophage-Derived Inflammatory Mediators
title_short TLR2, TLR4 and CD14 Recognize Venom-Associated Molecular Patterns from Tityus serrulatus to Induce Macrophage-Derived Inflammatory Mediators
title_sort tlr2, tlr4 and cd14 recognize venom-associated molecular patterns from tityus serrulatus to induce macrophage-derived inflammatory mediators
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3917877/
https://www.ncbi.nlm.nih.gov/pubmed/24516606
http://dx.doi.org/10.1371/journal.pone.0088174
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