Unmixing of fluorescence spectra to resolve quantitative time-series measurements of gene expression in plate readers

BACKGROUND: To connect gene expression with cellular physiology, we need to follow levels of proteins over time. Experiments typically use variants of Green Fluorescent Protein (GFP), and time-series measurements require specialist expertise if single cells are to be followed. Fluorescence plate rea...

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Detalles Bibliográficos
Autores principales: Lichten, Catherine A, White, Rachel, Clark, Ivan BN, Swain, Peter S
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2014
Materias:
Methodology Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3917901/
https://www.ncbi.nlm.nih.gov/pubmed/24495318
http://dx.doi.org/10.1186/1472-6750-14-11

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