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A degenerate PCR-based strategy as a means of identifying homologues of aminoglycoside and β-lactam resistance genes in the gut microbiota
BACKGROUND: The potential for the human gut microbiota to serve as a reservoir for antibiotic resistance genes has been the subject of recent discussion. However, this has yet to be investigated using a rapid PCR-based approach. In light of this, here we aim to determine if degenerate PCR primers ca...
Autores principales: | Fouhy, Fiona, Ross, R Paul, Fitzgerald, Gerald F, Stanton, Catherine, Cotter, Paul D |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
BioMed Central
2014
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3917905/ https://www.ncbi.nlm.nih.gov/pubmed/24499167 http://dx.doi.org/10.1186/1471-2180-14-25 |
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