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Efficient DNA ligation in DNA–RNA hybrid helices by Chlorella virus DNA ligase

Single-stranded DNA molecules (ssDNA) annealed to an RNA splint are notoriously poor substrates for DNA ligases. Herein we report the unexpectedly efficient ligation of RNA-splinted DNA by Chlorella virus DNA ligase (PBCV-1 DNA ligase). PBCV-1 DNA ligase ligated ssDNA splinted by RNA with k(cat) ≈ 8...

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Detalles Bibliográficos
Autores principales:	Lohman, Gregory J. S., Zhang, Yinhua, Zhelkovsky, Alexander M., Cantor, Eric J., Evans, Thomas C.
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Oxford University Press 2014
Materias:	Nucleic Acid Enzymes
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3919565/ https://www.ncbi.nlm.nih.gov/pubmed/24203707 http://dx.doi.org/10.1093/nar/gkt1032

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