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Decreased Peripheral Natural Killer Cells Activity in the Immune Activated Stage of Chronic Hepatitis B

BACKGROUND & AIMS: The natural course of chronic hepatitis B virus (HBV) infection is characterized by different immune responses, ranging from immune tolerant (IT) to immune activated (IA) stages. In our study, we investigated the natural killer (NK) cells activity in patients at different immu...

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Autores principales: Li, Yuan, Wang, Jiu-Jun, Gao, Shan, Liu, Qian, Bai, Jia, Zhao, Xue-Qi, Hao, You-Hua, Ding, Hong-Hui, Zhu, Fan, Yang, Dong-Liang, Zhao, Xi-Ping
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2014
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3919705/
https://www.ncbi.nlm.nih.gov/pubmed/24520324
http://dx.doi.org/10.1371/journal.pone.0086927
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author Li, Yuan
Wang, Jiu-Jun
Gao, Shan
Liu, Qian
Bai, Jia
Zhao, Xue-Qi
Hao, You-Hua
Ding, Hong-Hui
Zhu, Fan
Yang, Dong-Liang
Zhao, Xi-Ping
author_facet Li, Yuan
Wang, Jiu-Jun
Gao, Shan
Liu, Qian
Bai, Jia
Zhao, Xue-Qi
Hao, You-Hua
Ding, Hong-Hui
Zhu, Fan
Yang, Dong-Liang
Zhao, Xi-Ping
author_sort Li, Yuan
collection PubMed
description BACKGROUND & AIMS: The natural course of chronic hepatitis B virus (HBV) infection is characterized by different immune responses, ranging from immune tolerant (IT) to immune activated (IA) stages. In our study, we investigated the natural killer (NK) cells activity in patients at different immunological stages of chronic HBV infection. METHODS: Blood samples obtained from 57 HBeAg positive patients with chronic hepatitis B (CHB), including 15 patients in the immune tolerant (IT) stage, 42 patients in the immune activated (IA) stage, and 18 healthy individuals (HI). The analyses included flow cytometry to detect NK cells, the determination of cytokine levels as well as of surface receptor expression and cytotoxicity. RESULTS: NK cells in peripheral blood were significantly lower in patients in the IA stage of CHB compared to HI (p<0.05). Patients in the IA stage of CHB had lower levels of NK cells activating receptor NKp30 and NKG2D expression, cytokine interferon-γ (IFN-γ) and tumor necrosis factor-α (TNF-α) production, as compared to patients in the IT stage and HI, respectively (p<0.05). Cytotoxicity of NK cells was lower in patients in the IA stage of CHB compared to patients in the IT stage and HI, respectively (p<0.05). The level of IFN-γ but not level of TNF-α and cytotoxicity of NK cells was inversely correlated with serum HBV load in patients with CHB. Peripheral NK cells activity did not correlate with ALT level. CONCLUSION: NK cells activity was lower in CHB patients, especially in those in the IA stage.
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spelling pubmed-39197052014-02-11 Decreased Peripheral Natural Killer Cells Activity in the Immune Activated Stage of Chronic Hepatitis B Li, Yuan Wang, Jiu-Jun Gao, Shan Liu, Qian Bai, Jia Zhao, Xue-Qi Hao, You-Hua Ding, Hong-Hui Zhu, Fan Yang, Dong-Liang Zhao, Xi-Ping PLoS One Research Article BACKGROUND & AIMS: The natural course of chronic hepatitis B virus (HBV) infection is characterized by different immune responses, ranging from immune tolerant (IT) to immune activated (IA) stages. In our study, we investigated the natural killer (NK) cells activity in patients at different immunological stages of chronic HBV infection. METHODS: Blood samples obtained from 57 HBeAg positive patients with chronic hepatitis B (CHB), including 15 patients in the immune tolerant (IT) stage, 42 patients in the immune activated (IA) stage, and 18 healthy individuals (HI). The analyses included flow cytometry to detect NK cells, the determination of cytokine levels as well as of surface receptor expression and cytotoxicity. RESULTS: NK cells in peripheral blood were significantly lower in patients in the IA stage of CHB compared to HI (p<0.05). Patients in the IA stage of CHB had lower levels of NK cells activating receptor NKp30 and NKG2D expression, cytokine interferon-γ (IFN-γ) and tumor necrosis factor-α (TNF-α) production, as compared to patients in the IT stage and HI, respectively (p<0.05). Cytotoxicity of NK cells was lower in patients in the IA stage of CHB compared to patients in the IT stage and HI, respectively (p<0.05). The level of IFN-γ but not level of TNF-α and cytotoxicity of NK cells was inversely correlated with serum HBV load in patients with CHB. Peripheral NK cells activity did not correlate with ALT level. CONCLUSION: NK cells activity was lower in CHB patients, especially in those in the IA stage. Public Library of Science 2014-02-10 /pmc/articles/PMC3919705/ /pubmed/24520324 http://dx.doi.org/10.1371/journal.pone.0086927 Text en © 2014 Li et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
spellingShingle Research Article
Li, Yuan
Wang, Jiu-Jun
Gao, Shan
Liu, Qian
Bai, Jia
Zhao, Xue-Qi
Hao, You-Hua
Ding, Hong-Hui
Zhu, Fan
Yang, Dong-Liang
Zhao, Xi-Ping
Decreased Peripheral Natural Killer Cells Activity in the Immune Activated Stage of Chronic Hepatitis B
title Decreased Peripheral Natural Killer Cells Activity in the Immune Activated Stage of Chronic Hepatitis B
title_full Decreased Peripheral Natural Killer Cells Activity in the Immune Activated Stage of Chronic Hepatitis B
title_fullStr Decreased Peripheral Natural Killer Cells Activity in the Immune Activated Stage of Chronic Hepatitis B
title_full_unstemmed Decreased Peripheral Natural Killer Cells Activity in the Immune Activated Stage of Chronic Hepatitis B
title_short Decreased Peripheral Natural Killer Cells Activity in the Immune Activated Stage of Chronic Hepatitis B
title_sort decreased peripheral natural killer cells activity in the immune activated stage of chronic hepatitis b
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3919705/
https://www.ncbi.nlm.nih.gov/pubmed/24520324
http://dx.doi.org/10.1371/journal.pone.0086927
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