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The Influence of Chemical Chaperones on Enzymatic Activity under Thermal and Chemical Stresses: Common Features and Variation among Diverse Chemical Families

Molecular and chemical chaperones are key components of the two main mechanisms that ensure structural stability and activity under environmental stresses. Yet, chemical chaperones are often regarded only as osmolytes and their role beyond osmotic regulation is not fully understood. Here, we systema...

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Autores principales: Levy-Sakin, Michal, Berger, Or, Feibish, Nir, Sharon, Noa, Schnaider, Lee, Shmul, Guy, Amir, Yaniv, Buzhansky, Ludmila, Gazit, Ehud
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2014
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3919781/
https://www.ncbi.nlm.nih.gov/pubmed/24520396
http://dx.doi.org/10.1371/journal.pone.0088541
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author Levy-Sakin, Michal
Berger, Or
Feibish, Nir
Sharon, Noa
Schnaider, Lee
Shmul, Guy
Amir, Yaniv
Buzhansky, Ludmila
Gazit, Ehud
author_facet Levy-Sakin, Michal
Berger, Or
Feibish, Nir
Sharon, Noa
Schnaider, Lee
Shmul, Guy
Amir, Yaniv
Buzhansky, Ludmila
Gazit, Ehud
author_sort Levy-Sakin, Michal
collection PubMed
description Molecular and chemical chaperones are key components of the two main mechanisms that ensure structural stability and activity under environmental stresses. Yet, chemical chaperones are often regarded only as osmolytes and their role beyond osmotic regulation is not fully understood. Here, we systematically studied a large group of chemical chaperones, representatives of diverse chemical families, for their protective influence under either thermal or chemical stresses. Consistent with previous studies, we observed that in spite of the structural similarity between sugars and sugar alcohols, they have an apparent difference in their protective potential. Our results support the notion that the protective activity is mediated by the solvent and the presence of water is essential. In the current work we revealed that i) polyols and sugars have a completely different profile of protective activity toward trifluoroethanol and thermal stress; ii) minor changes in solvent composition that do not affect enzyme activity, yet have a great effect on the ability of osmolytes to act as protectants and iii) increasing the number of active groups of carbohydrates makes them better protectants while increasing the number of active groups of methylamines does not, as revealed by attempts to synthesize de novo designed methylamines with multiple functional groups.
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spelling pubmed-39197812014-02-11 The Influence of Chemical Chaperones on Enzymatic Activity under Thermal and Chemical Stresses: Common Features and Variation among Diverse Chemical Families Levy-Sakin, Michal Berger, Or Feibish, Nir Sharon, Noa Schnaider, Lee Shmul, Guy Amir, Yaniv Buzhansky, Ludmila Gazit, Ehud PLoS One Research Article Molecular and chemical chaperones are key components of the two main mechanisms that ensure structural stability and activity under environmental stresses. Yet, chemical chaperones are often regarded only as osmolytes and their role beyond osmotic regulation is not fully understood. Here, we systematically studied a large group of chemical chaperones, representatives of diverse chemical families, for their protective influence under either thermal or chemical stresses. Consistent with previous studies, we observed that in spite of the structural similarity between sugars and sugar alcohols, they have an apparent difference in their protective potential. Our results support the notion that the protective activity is mediated by the solvent and the presence of water is essential. In the current work we revealed that i) polyols and sugars have a completely different profile of protective activity toward trifluoroethanol and thermal stress; ii) minor changes in solvent composition that do not affect enzyme activity, yet have a great effect on the ability of osmolytes to act as protectants and iii) increasing the number of active groups of carbohydrates makes them better protectants while increasing the number of active groups of methylamines does not, as revealed by attempts to synthesize de novo designed methylamines with multiple functional groups. Public Library of Science 2014-02-10 /pmc/articles/PMC3919781/ /pubmed/24520396 http://dx.doi.org/10.1371/journal.pone.0088541 Text en © 2014 Levy-Sakin et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
spellingShingle Research Article
Levy-Sakin, Michal
Berger, Or
Feibish, Nir
Sharon, Noa
Schnaider, Lee
Shmul, Guy
Amir, Yaniv
Buzhansky, Ludmila
Gazit, Ehud
The Influence of Chemical Chaperones on Enzymatic Activity under Thermal and Chemical Stresses: Common Features and Variation among Diverse Chemical Families
title The Influence of Chemical Chaperones on Enzymatic Activity under Thermal and Chemical Stresses: Common Features and Variation among Diverse Chemical Families
title_full The Influence of Chemical Chaperones on Enzymatic Activity under Thermal and Chemical Stresses: Common Features and Variation among Diverse Chemical Families
title_fullStr The Influence of Chemical Chaperones on Enzymatic Activity under Thermal and Chemical Stresses: Common Features and Variation among Diverse Chemical Families
title_full_unstemmed The Influence of Chemical Chaperones on Enzymatic Activity under Thermal and Chemical Stresses: Common Features and Variation among Diverse Chemical Families
title_short The Influence of Chemical Chaperones on Enzymatic Activity under Thermal and Chemical Stresses: Common Features and Variation among Diverse Chemical Families
title_sort influence of chemical chaperones on enzymatic activity under thermal and chemical stresses: common features and variation among diverse chemical families
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3919781/
https://www.ncbi.nlm.nih.gov/pubmed/24520396
http://dx.doi.org/10.1371/journal.pone.0088541
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