Cargando…
Treadmill exercise reduces spinal cord injury-induced apoptosis by activating the PI3K/Akt pathway in rats
Apoptosis occurring secondary to spinal cord injury (SCI) causes further neural damage and functional loss. In this study, a rat model was used to investigate the effect of treadmill exercise on SCI-induced apoptosis and expression of neurotrophic factors. To produce SCI, a contusion injury (10 g ×...
Autores principales: | , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
D.A. Spandidos
2014
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3919853/ https://www.ncbi.nlm.nih.gov/pubmed/24520250 http://dx.doi.org/10.3892/etm.2013.1451 |
_version_ | 1782303096354897920 |
---|---|
author | JUNG, SUN-YOUNG KIM, DAE-YOUNG YUNE, TAE YOUNG SHIN, DONG-HOON BAEK, SANG-BIN KIM, CHANG-JU |
author_facet | JUNG, SUN-YOUNG KIM, DAE-YOUNG YUNE, TAE YOUNG SHIN, DONG-HOON BAEK, SANG-BIN KIM, CHANG-JU |
author_sort | JUNG, SUN-YOUNG |
collection | PubMed |
description | Apoptosis occurring secondary to spinal cord injury (SCI) causes further neural damage and functional loss. In this study, a rat model was used to investigate the effect of treadmill exercise on SCI-induced apoptosis and expression of neurotrophic factors. To produce SCI, a contusion injury (10 g × 25 mm) was applied subsequent to laminectomy at the T9–T10 level. Following SCI, treadmill exercise was performed for six weeks. Hindlimb motor function was evaluated with a grid-walking test. The expression of neurotrophic factors and the level of apoptosis at the site of SCI were determined by western blotting. SCI reduced hindlimb motor function and suppressed expression of neurotrophin (NT)-3 and insulin-like growth factor (IGF)-1. Expression of phosphatidylinositol 3-kinase (PI3K), the ratio of phosphorylated Akt to Akt (pAkt/Akt) and the ratio of B-cell lymphoma 2 (Bcl-2) to Bax (Bcl-2/Bax) were decreased, and cleaved caspase-3 expression was increased by SCI. Treadmill exercise enhanced hindlimb motor function and increased expression of nerve growth factor (NGF), NT-3 and IGF-1 in the SCI rats. Treadmill exercise increased PI3K expression, the pAkt/Akt and the Bcl-2/Bax ratios, and suppressed cleaved caspase-3 expression in the injured spinal cord. This study demonstrated that treadmill exercise promotes the recovery of motor function by suppressing apoptosis in the injured spinal cord. The beneficial effect of exercise may be attributed to the increase in expression of neurotrophic factors via activation of the PI3K/Akt pathway. |
format | Online Article Text |
id | pubmed-3919853 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2014 |
publisher | D.A. Spandidos |
record_format | MEDLINE/PubMed |
spelling | pubmed-39198532014-02-11 Treadmill exercise reduces spinal cord injury-induced apoptosis by activating the PI3K/Akt pathway in rats JUNG, SUN-YOUNG KIM, DAE-YOUNG YUNE, TAE YOUNG SHIN, DONG-HOON BAEK, SANG-BIN KIM, CHANG-JU Exp Ther Med Articles Apoptosis occurring secondary to spinal cord injury (SCI) causes further neural damage and functional loss. In this study, a rat model was used to investigate the effect of treadmill exercise on SCI-induced apoptosis and expression of neurotrophic factors. To produce SCI, a contusion injury (10 g × 25 mm) was applied subsequent to laminectomy at the T9–T10 level. Following SCI, treadmill exercise was performed for six weeks. Hindlimb motor function was evaluated with a grid-walking test. The expression of neurotrophic factors and the level of apoptosis at the site of SCI were determined by western blotting. SCI reduced hindlimb motor function and suppressed expression of neurotrophin (NT)-3 and insulin-like growth factor (IGF)-1. Expression of phosphatidylinositol 3-kinase (PI3K), the ratio of phosphorylated Akt to Akt (pAkt/Akt) and the ratio of B-cell lymphoma 2 (Bcl-2) to Bax (Bcl-2/Bax) were decreased, and cleaved caspase-3 expression was increased by SCI. Treadmill exercise enhanced hindlimb motor function and increased expression of nerve growth factor (NGF), NT-3 and IGF-1 in the SCI rats. Treadmill exercise increased PI3K expression, the pAkt/Akt and the Bcl-2/Bax ratios, and suppressed cleaved caspase-3 expression in the injured spinal cord. This study demonstrated that treadmill exercise promotes the recovery of motor function by suppressing apoptosis in the injured spinal cord. The beneficial effect of exercise may be attributed to the increase in expression of neurotrophic factors via activation of the PI3K/Akt pathway. D.A. Spandidos 2014-03 2013-12-17 /pmc/articles/PMC3919853/ /pubmed/24520250 http://dx.doi.org/10.3892/etm.2013.1451 Text en Copyright © 2014, Spandidos Publications http://creativecommons.org/licenses/by/3.0 This is an open-access article licensed under a Creative Commons Attribution-NonCommercial 3.0 Unported License. The article may be redistributed, reproduced, and reused for non-commercial purposes, provided the original source is properly cited. |
spellingShingle | Articles JUNG, SUN-YOUNG KIM, DAE-YOUNG YUNE, TAE YOUNG SHIN, DONG-HOON BAEK, SANG-BIN KIM, CHANG-JU Treadmill exercise reduces spinal cord injury-induced apoptosis by activating the PI3K/Akt pathway in rats |
title | Treadmill exercise reduces spinal cord injury-induced apoptosis by activating the PI3K/Akt pathway in rats |
title_full | Treadmill exercise reduces spinal cord injury-induced apoptosis by activating the PI3K/Akt pathway in rats |
title_fullStr | Treadmill exercise reduces spinal cord injury-induced apoptosis by activating the PI3K/Akt pathway in rats |
title_full_unstemmed | Treadmill exercise reduces spinal cord injury-induced apoptosis by activating the PI3K/Akt pathway in rats |
title_short | Treadmill exercise reduces spinal cord injury-induced apoptosis by activating the PI3K/Akt pathway in rats |
title_sort | treadmill exercise reduces spinal cord injury-induced apoptosis by activating the pi3k/akt pathway in rats |
topic | Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3919853/ https://www.ncbi.nlm.nih.gov/pubmed/24520250 http://dx.doi.org/10.3892/etm.2013.1451 |
work_keys_str_mv | AT jungsunyoung treadmillexercisereducesspinalcordinjuryinducedapoptosisbyactivatingthepi3kaktpathwayinrats AT kimdaeyoung treadmillexercisereducesspinalcordinjuryinducedapoptosisbyactivatingthepi3kaktpathwayinrats AT yunetaeyoung treadmillexercisereducesspinalcordinjuryinducedapoptosisbyactivatingthepi3kaktpathwayinrats AT shindonghoon treadmillexercisereducesspinalcordinjuryinducedapoptosisbyactivatingthepi3kaktpathwayinrats AT baeksangbin treadmillexercisereducesspinalcordinjuryinducedapoptosisbyactivatingthepi3kaktpathwayinrats AT kimchangju treadmillexercisereducesspinalcordinjuryinducedapoptosisbyactivatingthepi3kaktpathwayinrats |