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Determination of ctxAB expression in Vibrio cholerae Classical and El Tor strains using Real-Time PCR

Cholera is an infection of the small intestines caused by the bacterium V. cholerae. It is a major cause of health threat and also a major cause of death worldwide and especially in developing countries. The major virulence factor produced by V. cholerae during infection is the cholera toxin. Total...

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Detalles Bibliográficos
Autores principales: Amin Marashi, Seyed Mahmoud, Rajabnia, Ramazan, Imani Fooladi, Abbas Ali, Hojati, Zohreh, Moghim, Sharareh, Nasr Esfahani, Bahram
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Babol University of Medical Sciences 2013
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3920520/
https://www.ncbi.nlm.nih.gov/pubmed/24551784
Descripción
Sumario:Cholera is an infection of the small intestines caused by the bacterium V. cholerae. It is a major cause of health threat and also a major cause of death worldwide and especially in developing countries. The major virulence factor produced by V. cholerae during infection is the cholera toxin. Total mRNA extraction and reverse transcription was performed for making ctxAB cDNA. Relative Real-Time PCR analysis showed unequal enterotoxin production in V. cholerae strains. The results showed that, classical strain produces cholera toxin more than El Tor strain.