Determination of ctxAB expression in Vibrio cholerae Classical and El Tor strains using Real-Time PCR

Cholera is an infection of the small intestines caused by the bacterium V. cholerae. It is a major cause of health threat and also a major cause of death worldwide and especially in developing countries. The major virulence factor produced by V. cholerae during infection is the cholera toxin. Total...

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Autores principales: Amin Marashi, Seyed Mahmoud, Rajabnia, Ramazan, Imani Fooladi, Abbas Ali, Hojati, Zohreh, Moghim, Sharareh, Nasr Esfahani, Bahram
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Babol University of Medical Sciences 2013
Materias:
Original Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3920520/
https://www.ncbi.nlm.nih.gov/pubmed/24551784
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author Amin Marashi, Seyed Mahmoud
Rajabnia, Ramazan
Imani Fooladi, Abbas Ali
Hojati, Zohreh
Moghim, Sharareh
Nasr Esfahani, Bahram
author_facet Amin Marashi, Seyed Mahmoud
Rajabnia, Ramazan
Imani Fooladi, Abbas Ali
Hojati, Zohreh
Moghim, Sharareh
Nasr Esfahani, Bahram
author_sort Amin Marashi, Seyed Mahmoud
collection PubMed
description Cholera is an infection of the small intestines caused by the bacterium V. cholerae. It is a major cause of health threat and also a major cause of death worldwide and especially in developing countries. The major virulence factor produced by V. cholerae during infection is the cholera toxin. Total mRNA extraction and reverse transcription was performed for making ctxAB cDNA. Relative Real-Time PCR analysis showed unequal enterotoxin production in V. cholerae strains. The results showed that, classical strain produces cholera toxin more than El Tor strain.
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spelling pubmed-39205202014-02-18 Determination of ctxAB expression in Vibrio cholerae Classical and El Tor strains using Real-Time PCR Amin Marashi, Seyed Mahmoud Rajabnia, Ramazan Imani Fooladi, Abbas Ali Hojati, Zohreh Moghim, Sharareh Nasr Esfahani, Bahram Int J Mol Cell Med Original Article Cholera is an infection of the small intestines caused by the bacterium V. cholerae. It is a major cause of health threat and also a major cause of death worldwide and especially in developing countries. The major virulence factor produced by V. cholerae during infection is the cholera toxin. Total mRNA extraction and reverse transcription was performed for making ctxAB cDNA. Relative Real-Time PCR analysis showed unequal enterotoxin production in V. cholerae strains. The results showed that, classical strain produces cholera toxin more than El Tor strain. Babol University of Medical Sciences 2013 /pmc/articles/PMC3920520/ /pubmed/24551784 Text en © 2013, International Journal of Molecular and Cellular Medicine This is an Open Access article distributed under the terms of the Creative Commons Attribution License, (http://creativecommons.org/licenses/by/3.0/) which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Original Article
Amin Marashi, Seyed Mahmoud
Rajabnia, Ramazan
Imani Fooladi, Abbas Ali
Hojati, Zohreh
Moghim, Sharareh
Nasr Esfahani, Bahram
Determination of ctxAB expression in Vibrio cholerae Classical and El Tor strains using Real-Time PCR
title Determination of ctxAB expression in Vibrio cholerae Classical and El Tor strains using Real-Time PCR
title_full Determination of ctxAB expression in Vibrio cholerae Classical and El Tor strains using Real-Time PCR
title_fullStr Determination of ctxAB expression in Vibrio cholerae Classical and El Tor strains using Real-Time PCR
title_full_unstemmed Determination of ctxAB expression in Vibrio cholerae Classical and El Tor strains using Real-Time PCR
title_short Determination of ctxAB expression in Vibrio cholerae Classical and El Tor strains using Real-Time PCR
title_sort determination of ctxab expression in vibrio cholerae classical and el tor strains using real-time pcr
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3920520/
https://www.ncbi.nlm.nih.gov/pubmed/24551784
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