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Resistin and Visfatin Expression in HCT-116 Colorectal Cancer Cell Line
Adipocytokines, hormones secreted from adipose tissue, have been shown to be associated with many cancers such as breast, prostate and colorectal cancer. Recent studies have indicated that resistin and visfatin, two of these adipokines have high level plasma concentrations in colorectal cancer patie...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Babol University of Medical Sciences
2013
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3920537/ https://www.ncbi.nlm.nih.gov/pubmed/24551805 |
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author | Ghaemmaghami, Sara Mohaddes, Seyed Mojtaba Hedayati, Mehdi Gorgian Mohammadi, Masumeh Dehbashi, Golnoosh |
author_facet | Ghaemmaghami, Sara Mohaddes, Seyed Mojtaba Hedayati, Mehdi Gorgian Mohammadi, Masumeh Dehbashi, Golnoosh |
author_sort | Ghaemmaghami, Sara |
collection | PubMed |
description | Adipocytokines, hormones secreted from adipose tissue, have been shown to be associated with many cancers such as breast, prostate and colorectal cancer. Recent studies have indicated that resistin and visfatin, two of these adipokines have high level plasma concentrations in colorectal cancer patients and may be promising biomarkers for colorectal cancer. The aim of this study was to identify whether the colorectal cancer cell line, HCT-116, itself is the source of these two adipokines secretion. Resistin and visfatin expression were investigated in HCT-116 by RT – PCR at mRNA level and confirmed by ELISA at protein level. Visfatin showed a high expression at both mRNA and protein levels in HCT-116. Conversely, resistin was not expressed in either cell lysate or supernatant. These results showed that HCT-116 colorectal cancer cells secrete and express visfatin endogenously. However, they are not the main source of resistin and the high level of resistin in colorectal cancer may be due to monocytes and other inflammatory cells which increase in proinflammation status of cancer. Taken together, visfatin may act on colorectal cancer cell in an autocrine manner while resistin may act in a paracrine manner. |
format | Online Article Text |
id | pubmed-3920537 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2013 |
publisher | Babol University of Medical Sciences |
record_format | MEDLINE/PubMed |
spelling | pubmed-39205372014-02-18 Resistin and Visfatin Expression in HCT-116 Colorectal Cancer Cell Line Ghaemmaghami, Sara Mohaddes, Seyed Mojtaba Hedayati, Mehdi Gorgian Mohammadi, Masumeh Dehbashi, Golnoosh Int J Mol Cell Med Original Article Adipocytokines, hormones secreted from adipose tissue, have been shown to be associated with many cancers such as breast, prostate and colorectal cancer. Recent studies have indicated that resistin and visfatin, two of these adipokines have high level plasma concentrations in colorectal cancer patients and may be promising biomarkers for colorectal cancer. The aim of this study was to identify whether the colorectal cancer cell line, HCT-116, itself is the source of these two adipokines secretion. Resistin and visfatin expression were investigated in HCT-116 by RT – PCR at mRNA level and confirmed by ELISA at protein level. Visfatin showed a high expression at both mRNA and protein levels in HCT-116. Conversely, resistin was not expressed in either cell lysate or supernatant. These results showed that HCT-116 colorectal cancer cells secrete and express visfatin endogenously. However, they are not the main source of resistin and the high level of resistin in colorectal cancer may be due to monocytes and other inflammatory cells which increase in proinflammation status of cancer. Taken together, visfatin may act on colorectal cancer cell in an autocrine manner while resistin may act in a paracrine manner. Babol University of Medical Sciences 2013 /pmc/articles/PMC3920537/ /pubmed/24551805 Text en © 2013, International Journal of Molecular and Cellular Medicine This is an Open Access article distributed under the terms of the Creative Commons Attribution License, (http://creativecommons.org/licenses/by/3.0/) which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Original Article Ghaemmaghami, Sara Mohaddes, Seyed Mojtaba Hedayati, Mehdi Gorgian Mohammadi, Masumeh Dehbashi, Golnoosh Resistin and Visfatin Expression in HCT-116 Colorectal Cancer Cell Line |
title | Resistin and Visfatin Expression in HCT-116 Colorectal Cancer Cell Line |
title_full | Resistin and Visfatin Expression in HCT-116 Colorectal Cancer Cell Line |
title_fullStr | Resistin and Visfatin Expression in HCT-116 Colorectal Cancer Cell Line |
title_full_unstemmed | Resistin and Visfatin Expression in HCT-116 Colorectal Cancer Cell Line |
title_short | Resistin and Visfatin Expression in HCT-116 Colorectal Cancer Cell Line |
title_sort | resistin and visfatin expression in hct-116 colorectal cancer cell line |
topic | Original Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3920537/ https://www.ncbi.nlm.nih.gov/pubmed/24551805 |
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