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Protective Effect of Pomegranate Seed Oil Against Acute Toxicity of Diazinon in Rat Kidney

Diazinon is an organophosphate which is extensively used in trade and agriculture. Due to its widespread application, its toxicity is common. Several studies have shown that organophosphates are able to induce oxidative stress by generating free radicals and depletion of endogenous antioxidants. Pom...

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Autores principales: Boroushaki, Mohammad Taher, Arshadi, Delnia, Jalili-Rasti, Hamideh, Asadpour, Elham, Hosseini, Azar
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Shaheed Beheshti University of Medical Sciences 2013
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3920704/
https://www.ncbi.nlm.nih.gov/pubmed/24523762
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author Boroushaki, Mohammad Taher
Arshadi, Delnia
Jalili-Rasti, Hamideh
Asadpour, Elham
Hosseini, Azar
author_facet Boroushaki, Mohammad Taher
Arshadi, Delnia
Jalili-Rasti, Hamideh
Asadpour, Elham
Hosseini, Azar
author_sort Boroushaki, Mohammad Taher
collection PubMed
description Diazinon is an organophosphate which is extensively used in trade and agriculture. Due to its widespread application, its toxicity is common. Several studies have shown that organophosphates are able to induce oxidative stress by generating free radicals and depletion of endogenous antioxidants. Pomegranate seed oil (PSO) possesses anti-inflammatory and antioxidant effects. In this study, the effect of PSO was evaluated on diazinon-induced nephrotoxicity in rat. Wistar male rats were randomly divided into four groups, 6 each. Group one received saline, 1 mL/kg, group 2 received diazinon 100 mg/kg. Groups 3 and 4 received PSO, 0.32 and 0.64 mg/kg, one hour before diazinon 100 mg/kg respectively. After 24 h, animals were anesthetized. Blood samples were taken out by cardiac puncture for measuring the level of serum urea and creatinine. 24 h urine samples were also collected for measuring glucose and protein concentration. The right kidney was removed and homogenized for measuring malondialdehyde and thiol content Compare to control group, DIZ increased urea and serum creatinine, urinary glucose, and malondialdehyde, but did not modify significantly urinary protein and thiol content. In groups received PSO+ DIZ, serum creatinine, urinary glucose and MDA were significantly decreased. DIZ induced acute nephrotoxicity and oxidative stress. Probably, increasing of serum creatinine and urinary glucose are appropriate markers for diagnosis of kidney damage. In addition increasing of MDA level emphasizes that DIZ plays role in pathogenesis of kidney via oxidative stress mechanism. PSO reduced DIZ toxicity by antioxidant activity.
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spelling pubmed-39207042014-02-12 Protective Effect of Pomegranate Seed Oil Against Acute Toxicity of Diazinon in Rat Kidney Boroushaki, Mohammad Taher Arshadi, Delnia Jalili-Rasti, Hamideh Asadpour, Elham Hosseini, Azar Iran J Pharm Res Original Article Diazinon is an organophosphate which is extensively used in trade and agriculture. Due to its widespread application, its toxicity is common. Several studies have shown that organophosphates are able to induce oxidative stress by generating free radicals and depletion of endogenous antioxidants. Pomegranate seed oil (PSO) possesses anti-inflammatory and antioxidant effects. In this study, the effect of PSO was evaluated on diazinon-induced nephrotoxicity in rat. Wistar male rats were randomly divided into four groups, 6 each. Group one received saline, 1 mL/kg, group 2 received diazinon 100 mg/kg. Groups 3 and 4 received PSO, 0.32 and 0.64 mg/kg, one hour before diazinon 100 mg/kg respectively. After 24 h, animals were anesthetized. Blood samples were taken out by cardiac puncture for measuring the level of serum urea and creatinine. 24 h urine samples were also collected for measuring glucose and protein concentration. The right kidney was removed and homogenized for measuring malondialdehyde and thiol content Compare to control group, DIZ increased urea and serum creatinine, urinary glucose, and malondialdehyde, but did not modify significantly urinary protein and thiol content. In groups received PSO+ DIZ, serum creatinine, urinary glucose and MDA were significantly decreased. DIZ induced acute nephrotoxicity and oxidative stress. Probably, increasing of serum creatinine and urinary glucose are appropriate markers for diagnosis of kidney damage. In addition increasing of MDA level emphasizes that DIZ plays role in pathogenesis of kidney via oxidative stress mechanism. PSO reduced DIZ toxicity by antioxidant activity. Shaheed Beheshti University of Medical Sciences 2013 /pmc/articles/PMC3920704/ /pubmed/24523762 Text en © 2013 by School of Pharmacy, Shaheed Beheshti University of Medical Sciences and Health Services This is an Open Access article distributed under the terms of the Creative Commons Attribution License, (http://creativecommons.org/licenses/by/3.0/) which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Original Article
Boroushaki, Mohammad Taher
Arshadi, Delnia
Jalili-Rasti, Hamideh
Asadpour, Elham
Hosseini, Azar
Protective Effect of Pomegranate Seed Oil Against Acute Toxicity of Diazinon in Rat Kidney
title Protective Effect of Pomegranate Seed Oil Against Acute Toxicity of Diazinon in Rat Kidney
title_full Protective Effect of Pomegranate Seed Oil Against Acute Toxicity of Diazinon in Rat Kidney
title_fullStr Protective Effect of Pomegranate Seed Oil Against Acute Toxicity of Diazinon in Rat Kidney
title_full_unstemmed Protective Effect of Pomegranate Seed Oil Against Acute Toxicity of Diazinon in Rat Kidney
title_short Protective Effect of Pomegranate Seed Oil Against Acute Toxicity of Diazinon in Rat Kidney
title_sort protective effect of pomegranate seed oil against acute toxicity of diazinon in rat kidney
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3920704/
https://www.ncbi.nlm.nih.gov/pubmed/24523762
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