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Vinculin Binding Angle in Podosomes Revealed by High Resolution Microscopy

Podosomes are highly dynamic actin-rich adhesive structures formed predominantly by cells of the monocytic lineage, which degrade the extracellular matrix. They consist of a core of F-actin and actin-regulating proteins, surrounded by a ring of adhesion-associated proteins such as vinculin. We have...

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Autores principales: Walde, Marie, Monypenny, James, Heintzmann, Rainer, Jones, Gareth E., Cox, Susan
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2014
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3921150/
https://www.ncbi.nlm.nih.gov/pubmed/24523880
http://dx.doi.org/10.1371/journal.pone.0088251
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author Walde, Marie
Monypenny, James
Heintzmann, Rainer
Jones, Gareth E.
Cox, Susan
author_facet Walde, Marie
Monypenny, James
Heintzmann, Rainer
Jones, Gareth E.
Cox, Susan
author_sort Walde, Marie
collection PubMed
description Podosomes are highly dynamic actin-rich adhesive structures formed predominantly by cells of the monocytic lineage, which degrade the extracellular matrix. They consist of a core of F-actin and actin-regulating proteins, surrounded by a ring of adhesion-associated proteins such as vinculin. We have characterised the structure of podosomes in macrophages, particularly the structure of the ring, using three super-resolution fluorescence microscopy techniques: stimulated emission depletion microscopy, structured illumination microscopy and localisation microscopy. Rather than being round, as previously assumed, we found the vinculin ring to be created from relatively straight strands of vinculin, resulting in a distinctly polygonal shape. The strands bind preferentially at angles between 116° and 135°. Furthermore, adjacent vinculin strands are observed nucleating at the corners of the podosomes, suggesting a mechanism for podosome growth.
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spelling pubmed-39211502014-02-12 Vinculin Binding Angle in Podosomes Revealed by High Resolution Microscopy Walde, Marie Monypenny, James Heintzmann, Rainer Jones, Gareth E. Cox, Susan PLoS One Research Article Podosomes are highly dynamic actin-rich adhesive structures formed predominantly by cells of the monocytic lineage, which degrade the extracellular matrix. They consist of a core of F-actin and actin-regulating proteins, surrounded by a ring of adhesion-associated proteins such as vinculin. We have characterised the structure of podosomes in macrophages, particularly the structure of the ring, using three super-resolution fluorescence microscopy techniques: stimulated emission depletion microscopy, structured illumination microscopy and localisation microscopy. Rather than being round, as previously assumed, we found the vinculin ring to be created from relatively straight strands of vinculin, resulting in a distinctly polygonal shape. The strands bind preferentially at angles between 116° and 135°. Furthermore, adjacent vinculin strands are observed nucleating at the corners of the podosomes, suggesting a mechanism for podosome growth. Public Library of Science 2014-02-11 /pmc/articles/PMC3921150/ /pubmed/24523880 http://dx.doi.org/10.1371/journal.pone.0088251 Text en © 2014 Walde et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
spellingShingle Research Article
Walde, Marie
Monypenny, James
Heintzmann, Rainer
Jones, Gareth E.
Cox, Susan
Vinculin Binding Angle in Podosomes Revealed by High Resolution Microscopy
title Vinculin Binding Angle in Podosomes Revealed by High Resolution Microscopy
title_full Vinculin Binding Angle in Podosomes Revealed by High Resolution Microscopy
title_fullStr Vinculin Binding Angle in Podosomes Revealed by High Resolution Microscopy
title_full_unstemmed Vinculin Binding Angle in Podosomes Revealed by High Resolution Microscopy
title_short Vinculin Binding Angle in Podosomes Revealed by High Resolution Microscopy
title_sort vinculin binding angle in podosomes revealed by high resolution microscopy
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3921150/
https://www.ncbi.nlm.nih.gov/pubmed/24523880
http://dx.doi.org/10.1371/journal.pone.0088251
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