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Phylogenetic Analysis of a Swine Influenza A(H3N2) Virus Isolated in Korea in 2012
Influenza A virus (IAV) can infect avian and mammalian species, including humans. The genome nature of IAVs may contribute to viral adaptation in different animal hosts, resulting in gene reassortment and the reproduction of variants with optimal fitness. As seen again in the 2009 swine-origin influ...
Autores principales: | , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2014
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3921248/ https://www.ncbi.nlm.nih.gov/pubmed/24523938 http://dx.doi.org/10.1371/journal.pone.0088782 |
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author | Kim, Jin Il Lee, Ilseob Park, Sehee Lee, Sangmoo Hwang, Min-Woong Bae, Joon-Yong Heo, Jun Kim, Donghwan Jang, Seok-Il Kim, Kabsu Park, Man-Seong |
author_facet | Kim, Jin Il Lee, Ilseob Park, Sehee Lee, Sangmoo Hwang, Min-Woong Bae, Joon-Yong Heo, Jun Kim, Donghwan Jang, Seok-Il Kim, Kabsu Park, Man-Seong |
author_sort | Kim, Jin Il |
collection | PubMed |
description | Influenza A virus (IAV) can infect avian and mammalian species, including humans. The genome nature of IAVs may contribute to viral adaptation in different animal hosts, resulting in gene reassortment and the reproduction of variants with optimal fitness. As seen again in the 2009 swine-origin influenza A H1N1 pandemic, pigs are known to be susceptible to swine, avian, and human IAVs and can serve as a ‘mixing vessel’ for the generation of novel IAV variants. To this end, the emergence of swine influenza viruses must be kept under close surveillance. Herein, we report the isolation and phylogenetic study of a swine IAV, A/swine/Korea/PL01/2012 (swPL01, H3N2 subtype). After screening nasopharyngeal samples from pigs in the Gyeongsangnam-do region of Korea from December 2011 to May 2012, we isolated the swPL01 virus and sequenced its all of 8 genome segments (polymerase basic 2, PB2; polymerase basic 1, PB1; polymerase acidic, PA; hemagglutinin, HA; nucleocapsid protein, NP; neuraminidase, NA; matrix protein, M; and nonstructural protein, NS). The phylogenetic study, analyzed with reference strains registered in the National Center for Biotechnology Information (NCBI) database, indicated that the swPL01 virus was similar to the North American triple-reassortant swine strains and that the HA gene of the swPL01 virus was categorized into swine H3 cluster IV. The swPL01 virus had the M gene of the triple-reassortant swine H3N2 viruses, whereas that of other contemporary strains in Korea was transferred from the 2009 pandemic H1N1 virus. These data suggest the possibility that various swine H3N2 viruses may co-circulate in Korea, which underlines the importance of a sustained surveillance system against swine IAVs. |
format | Online Article Text |
id | pubmed-3921248 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2014 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-39212482014-02-12 Phylogenetic Analysis of a Swine Influenza A(H3N2) Virus Isolated in Korea in 2012 Kim, Jin Il Lee, Ilseob Park, Sehee Lee, Sangmoo Hwang, Min-Woong Bae, Joon-Yong Heo, Jun Kim, Donghwan Jang, Seok-Il Kim, Kabsu Park, Man-Seong PLoS One Research Article Influenza A virus (IAV) can infect avian and mammalian species, including humans. The genome nature of IAVs may contribute to viral adaptation in different animal hosts, resulting in gene reassortment and the reproduction of variants with optimal fitness. As seen again in the 2009 swine-origin influenza A H1N1 pandemic, pigs are known to be susceptible to swine, avian, and human IAVs and can serve as a ‘mixing vessel’ for the generation of novel IAV variants. To this end, the emergence of swine influenza viruses must be kept under close surveillance. Herein, we report the isolation and phylogenetic study of a swine IAV, A/swine/Korea/PL01/2012 (swPL01, H3N2 subtype). After screening nasopharyngeal samples from pigs in the Gyeongsangnam-do region of Korea from December 2011 to May 2012, we isolated the swPL01 virus and sequenced its all of 8 genome segments (polymerase basic 2, PB2; polymerase basic 1, PB1; polymerase acidic, PA; hemagglutinin, HA; nucleocapsid protein, NP; neuraminidase, NA; matrix protein, M; and nonstructural protein, NS). The phylogenetic study, analyzed with reference strains registered in the National Center for Biotechnology Information (NCBI) database, indicated that the swPL01 virus was similar to the North American triple-reassortant swine strains and that the HA gene of the swPL01 virus was categorized into swine H3 cluster IV. The swPL01 virus had the M gene of the triple-reassortant swine H3N2 viruses, whereas that of other contemporary strains in Korea was transferred from the 2009 pandemic H1N1 virus. These data suggest the possibility that various swine H3N2 viruses may co-circulate in Korea, which underlines the importance of a sustained surveillance system against swine IAVs. Public Library of Science 2014-02-11 /pmc/articles/PMC3921248/ /pubmed/24523938 http://dx.doi.org/10.1371/journal.pone.0088782 Text en © 2014 Kim et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited. |
spellingShingle | Research Article Kim, Jin Il Lee, Ilseob Park, Sehee Lee, Sangmoo Hwang, Min-Woong Bae, Joon-Yong Heo, Jun Kim, Donghwan Jang, Seok-Il Kim, Kabsu Park, Man-Seong Phylogenetic Analysis of a Swine Influenza A(H3N2) Virus Isolated in Korea in 2012 |
title | Phylogenetic Analysis of a Swine Influenza A(H3N2) Virus Isolated in Korea in 2012 |
title_full | Phylogenetic Analysis of a Swine Influenza A(H3N2) Virus Isolated in Korea in 2012 |
title_fullStr | Phylogenetic Analysis of a Swine Influenza A(H3N2) Virus Isolated in Korea in 2012 |
title_full_unstemmed | Phylogenetic Analysis of a Swine Influenza A(H3N2) Virus Isolated in Korea in 2012 |
title_short | Phylogenetic Analysis of a Swine Influenza A(H3N2) Virus Isolated in Korea in 2012 |
title_sort | phylogenetic analysis of a swine influenza a(h3n2) virus isolated in korea in 2012 |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3921248/ https://www.ncbi.nlm.nih.gov/pubmed/24523938 http://dx.doi.org/10.1371/journal.pone.0088782 |
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