Generation of TALEN-Mediated GR(dim) Knock-In Rats by Homologous Recombination

Transcription Activator-Like Effector Nucleases (TALEN) are potential tools for precise genome engineering of laboratory animals. We report the first targeted genomic integration in the rat using TALENs (Transcription Activator-Like Effector Nucleases) by homology-derived recombination (HDR). We ass...

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Autores principales: Ponce de León, Verónica, Mérillat, Anne-Marie, Tesson, Laurent, Anegón, Ignacio, Hummler, Edith
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2014
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3921256/
https://www.ncbi.nlm.nih.gov/pubmed/24523878
http://dx.doi.org/10.1371/journal.pone.0088146
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author Ponce de León, Verónica
Mérillat, Anne-Marie
Tesson, Laurent
Anegón, Ignacio
Hummler, Edith
author_facet Ponce de León, Verónica
Mérillat, Anne-Marie
Tesson, Laurent
Anegón, Ignacio
Hummler, Edith
author_sort Ponce de León, Verónica
collection PubMed
description Transcription Activator-Like Effector Nucleases (TALEN) are potential tools for precise genome engineering of laboratory animals. We report the first targeted genomic integration in the rat using TALENs (Transcription Activator-Like Effector Nucleases) by homology-derived recombination (HDR). We assembled TALENs and designed a linear donor insert targeting a pA476T mutation in the rat Glucocorticoid Receptor (Nr3c1) namely GR(dim), that prevents receptor homodimerization in the mouse. TALEN mRNA and linear double-stranded donor were microinjected into rat one-cell embryos. Overall, we observed targeted genomic modifications in 17% of the offspring, indicating high TALEN cutting efficiency in rat zygotes.
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spelling pubmed-39212562014-02-12 Generation of TALEN-Mediated GR(dim) Knock-In Rats by Homologous Recombination Ponce de León, Verónica Mérillat, Anne-Marie Tesson, Laurent Anegón, Ignacio Hummler, Edith PLoS One Research Article Transcription Activator-Like Effector Nucleases (TALEN) are potential tools for precise genome engineering of laboratory animals. We report the first targeted genomic integration in the rat using TALENs (Transcription Activator-Like Effector Nucleases) by homology-derived recombination (HDR). We assembled TALENs and designed a linear donor insert targeting a pA476T mutation in the rat Glucocorticoid Receptor (Nr3c1) namely GR(dim), that prevents receptor homodimerization in the mouse. TALEN mRNA and linear double-stranded donor were microinjected into rat one-cell embryos. Overall, we observed targeted genomic modifications in 17% of the offspring, indicating high TALEN cutting efficiency in rat zygotes. Public Library of Science 2014-02-11 /pmc/articles/PMC3921256/ /pubmed/24523878 http://dx.doi.org/10.1371/journal.pone.0088146 Text en © 2014 Ponce de Leon et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
spellingShingle Research Article
Ponce de León, Verónica
Mérillat, Anne-Marie
Tesson, Laurent
Anegón, Ignacio
Hummler, Edith
Generation of TALEN-Mediated GR(dim) Knock-In Rats by Homologous Recombination
title Generation of TALEN-Mediated GR(dim) Knock-In Rats by Homologous Recombination
title_full Generation of TALEN-Mediated GR(dim) Knock-In Rats by Homologous Recombination
title_fullStr Generation of TALEN-Mediated GR(dim) Knock-In Rats by Homologous Recombination
title_full_unstemmed Generation of TALEN-Mediated GR(dim) Knock-In Rats by Homologous Recombination
title_short Generation of TALEN-Mediated GR(dim) Knock-In Rats by Homologous Recombination
title_sort generation of talen-mediated gr(dim) knock-in rats by homologous recombination
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3921256/
https://www.ncbi.nlm.nih.gov/pubmed/24523878
http://dx.doi.org/10.1371/journal.pone.0088146
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