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Effects of pentoxifylline on renal structure after urethral obstruction in rat: A stereological study

OBJECTIVE: Chronic renal failure due to tubulointerstitial fibrosis is one of complications of lower urinary tract obstruction. Since pentoxifylline is a xanthine derivative that inhibits some inflammatory mediators, we conducted this study to investigate whether pentoxifylline inhibits renal fibros...

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Autores principales: Shirazi, Mehdi, Noorafshan, Ali, Farrokhi, Amir
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Polish Urological Association 2011
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3921697/
https://www.ncbi.nlm.nih.gov/pubmed/24578857
http://dx.doi.org/10.5173/ceju.2011.01.art6
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author Shirazi, Mehdi
Noorafshan, Ali
Farrokhi, Amir
author_facet Shirazi, Mehdi
Noorafshan, Ali
Farrokhi, Amir
author_sort Shirazi, Mehdi
collection PubMed
description OBJECTIVE: Chronic renal failure due to tubulointerstitial fibrosis is one of complications of lower urinary tract obstruction. Since pentoxifylline is a xanthine derivative that inhibits some inflammatory mediators, we conducted this study to investigate whether pentoxifylline inhibits renal fibrosis in a rat model of partial urethral obstruction (PUO). METHODS: All the rats underwent experimental PUO. Then, the animals were divided randomly into two groups: positive control group and experimental group. The experimental group received pentoxifylline 100 mg/kg per day via oral gavages for 4-weeks. The control group received the same dose of normal saline. After 4-weeks, all the rats underwent left nephrectomy. Kidney volume and weight and fractional and absolute volumes of the glomeruli, tubules, interstitium and vessels were determined with stereological methods. To reduce the workload of reference (kidney) volume estimation, the total kidney volume was determined after estimation of tissue shrinkage on isotropic uniform random histological sections. The total volume (amount) of each renal structure including fibrosis was estimated to avoid the bias conclusion due to relying on volume density alone. RESULTS: The absolute volume of interstitial fibrosis was lower in the experimental group (PUO with pentoxifylline treatment) (~84%; p ≤0.006) in comparison with the control group (PUO with no treatment). CONCLUSION: Pentoxifylline reduces interstitial renal fibrosis after partial urethral obstruction in rats.
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spelling pubmed-39216972014-02-27 Effects of pentoxifylline on renal structure after urethral obstruction in rat: A stereological study Shirazi, Mehdi Noorafshan, Ali Farrokhi, Amir Cent European J Urol Basic Sciences OBJECTIVE: Chronic renal failure due to tubulointerstitial fibrosis is one of complications of lower urinary tract obstruction. Since pentoxifylline is a xanthine derivative that inhibits some inflammatory mediators, we conducted this study to investigate whether pentoxifylline inhibits renal fibrosis in a rat model of partial urethral obstruction (PUO). METHODS: All the rats underwent experimental PUO. Then, the animals were divided randomly into two groups: positive control group and experimental group. The experimental group received pentoxifylline 100 mg/kg per day via oral gavages for 4-weeks. The control group received the same dose of normal saline. After 4-weeks, all the rats underwent left nephrectomy. Kidney volume and weight and fractional and absolute volumes of the glomeruli, tubules, interstitium and vessels were determined with stereological methods. To reduce the workload of reference (kidney) volume estimation, the total kidney volume was determined after estimation of tissue shrinkage on isotropic uniform random histological sections. The total volume (amount) of each renal structure including fibrosis was estimated to avoid the bias conclusion due to relying on volume density alone. RESULTS: The absolute volume of interstitial fibrosis was lower in the experimental group (PUO with pentoxifylline treatment) (~84%; p ≤0.006) in comparison with the control group (PUO with no treatment). CONCLUSION: Pentoxifylline reduces interstitial renal fibrosis after partial urethral obstruction in rats. Polish Urological Association 2011-03-18 2011 /pmc/articles/PMC3921697/ /pubmed/24578857 http://dx.doi.org/10.5173/ceju.2011.01.art6 Text en Copyright by Polish Urological Association http://creativecommons.org/licenses/by-nc-nd/3.0/ This is an Open Access article distributed under the terms of the Creative Commons Attribution-Noncommercial 3.0 Unported License, permitting all non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Basic Sciences
Shirazi, Mehdi
Noorafshan, Ali
Farrokhi, Amir
Effects of pentoxifylline on renal structure after urethral obstruction in rat: A stereological study
title Effects of pentoxifylline on renal structure after urethral obstruction in rat: A stereological study
title_full Effects of pentoxifylline on renal structure after urethral obstruction in rat: A stereological study
title_fullStr Effects of pentoxifylline on renal structure after urethral obstruction in rat: A stereological study
title_full_unstemmed Effects of pentoxifylline on renal structure after urethral obstruction in rat: A stereological study
title_short Effects of pentoxifylline on renal structure after urethral obstruction in rat: A stereological study
title_sort effects of pentoxifylline on renal structure after urethral obstruction in rat: a stereological study
topic Basic Sciences
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3921697/
https://www.ncbi.nlm.nih.gov/pubmed/24578857
http://dx.doi.org/10.5173/ceju.2011.01.art6
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