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A glance at quality score: implication for de novo transcriptome reconstruction of Illumina reads

Downstream analyses of short-reads from next-generation sequencing platforms are often preceded by a pre-processing step that removes uncalled and wrongly called bases. Standard approaches rely on their associated base quality scores to retain the read or a portion of it when the score is above a pr...

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Detalles Bibliográficos
Autores principales: Mbandi, Stanley Kimbung, Hesse, Uljana, Rees, D. Jasper G., Christoffels, Alan
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2014
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3921913/
https://www.ncbi.nlm.nih.gov/pubmed/24575122
http://dx.doi.org/10.3389/fgene.2014.00017