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A glance at quality score: implication for de novo transcriptome reconstruction of Illumina reads
Downstream analyses of short-reads from next-generation sequencing platforms are often preceded by a pre-processing step that removes uncalled and wrongly called bases. Standard approaches rely on their associated base quality scores to retain the read or a portion of it when the score is above a pr...
Autores principales: | , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Frontiers Media S.A.
2014
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3921913/ https://www.ncbi.nlm.nih.gov/pubmed/24575122 http://dx.doi.org/10.3389/fgene.2014.00017 |