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In vitro differentiation of human mesenchymal stem cells to epithelial lineage

Our study examined whether human bone marrow-derived MSCs are able to differentiate, in vitro, into functional epithelial-like cells. MSCs were isolated from the sternum of 8 patients with different hematological disorders. The surface phenotype of these cells was characterized.To induce epithelial...

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Autores principales: Păunescu, Virgil, Deak, Erika, Herman, Diana, Siska, Ioana Raluca, T˘anasie, Gabriela, Bunu, Carmen, Anghel, Simona, Tatu, Calin A, Oprea, Tudor I, Henschler, Reinhard, Rüster, Brigitte, Bistrian, Roxana, Seifried, Erhard
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Blackwell Publishing Ltd 2007
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3922356/
https://www.ncbi.nlm.nih.gov/pubmed/17635641
http://dx.doi.org/10.1111/j.1582-4934.2007.00041.x
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author Păunescu, Virgil
Deak, Erika
Herman, Diana
Siska, Ioana Raluca
T˘anasie, Gabriela
Bunu, Carmen
Anghel, Simona
Tatu, Calin A
Oprea, Tudor I
Henschler, Reinhard
Rüster, Brigitte
Bistrian, Roxana
Seifried, Erhard
author_facet Păunescu, Virgil
Deak, Erika
Herman, Diana
Siska, Ioana Raluca
T˘anasie, Gabriela
Bunu, Carmen
Anghel, Simona
Tatu, Calin A
Oprea, Tudor I
Henschler, Reinhard
Rüster, Brigitte
Bistrian, Roxana
Seifried, Erhard
author_sort Păunescu, Virgil
collection PubMed
description Our study examined whether human bone marrow-derived MSCs are able to differentiate, in vitro, into functional epithelial-like cells. MSCs were isolated from the sternum of 8 patients with different hematological disorders. The surface phenotype of these cells was characterized.To induce epithelial differentiation, MSCs were cultured using Epidermal Growth Factor, Keratinocyte Growth Factor, Hepatocyte Growth Factor and Insulin-like growth Factor-II. Differentiated cells were further characterized both morphologically and functionally by their capacity to express markers with specificity for epithelial lineage. The expression of cytokeratin 19 was assessed by immunocytochemistry, and cytokeratin 18 was evaluated by quantitative RT-PCR (Taq-man). The data demonstrate that human MSCs isolated from human bone marrow can differentiate into epithelial-like cells and may thus serve as a cell source for tissue engineering and cell therapy of epithelial tissue.
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spelling pubmed-39223562015-04-27 In vitro differentiation of human mesenchymal stem cells to epithelial lineage Păunescu, Virgil Deak, Erika Herman, Diana Siska, Ioana Raluca T˘anasie, Gabriela Bunu, Carmen Anghel, Simona Tatu, Calin A Oprea, Tudor I Henschler, Reinhard Rüster, Brigitte Bistrian, Roxana Seifried, Erhard J Cell Mol Med Articles Our study examined whether human bone marrow-derived MSCs are able to differentiate, in vitro, into functional epithelial-like cells. MSCs were isolated from the sternum of 8 patients with different hematological disorders. The surface phenotype of these cells was characterized.To induce epithelial differentiation, MSCs were cultured using Epidermal Growth Factor, Keratinocyte Growth Factor, Hepatocyte Growth Factor and Insulin-like growth Factor-II. Differentiated cells were further characterized both morphologically and functionally by their capacity to express markers with specificity for epithelial lineage. The expression of cytokeratin 19 was assessed by immunocytochemistry, and cytokeratin 18 was evaluated by quantitative RT-PCR (Taq-man). The data demonstrate that human MSCs isolated from human bone marrow can differentiate into epithelial-like cells and may thus serve as a cell source for tissue engineering and cell therapy of epithelial tissue. Blackwell Publishing Ltd 2007-05 2007-07-13 /pmc/articles/PMC3922356/ /pubmed/17635641 http://dx.doi.org/10.1111/j.1582-4934.2007.00041.x Text en
spellingShingle Articles
Păunescu, Virgil
Deak, Erika
Herman, Diana
Siska, Ioana Raluca
T˘anasie, Gabriela
Bunu, Carmen
Anghel, Simona
Tatu, Calin A
Oprea, Tudor I
Henschler, Reinhard
Rüster, Brigitte
Bistrian, Roxana
Seifried, Erhard
In vitro differentiation of human mesenchymal stem cells to epithelial lineage
title In vitro differentiation of human mesenchymal stem cells to epithelial lineage
title_full In vitro differentiation of human mesenchymal stem cells to epithelial lineage
title_fullStr In vitro differentiation of human mesenchymal stem cells to epithelial lineage
title_full_unstemmed In vitro differentiation of human mesenchymal stem cells to epithelial lineage
title_short In vitro differentiation of human mesenchymal stem cells to epithelial lineage
title_sort in vitro differentiation of human mesenchymal stem cells to epithelial lineage
topic Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3922356/
https://www.ncbi.nlm.nih.gov/pubmed/17635641
http://dx.doi.org/10.1111/j.1582-4934.2007.00041.x
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