Cargando…

Treadmill Exercise Training Prevents Myocardial Mechanical Dysfunction Induced by Androgenic-Anabolic Steroid Treatment in Rats

Elevated concentrations of testosterone and its synthetic analogs may induce changes in cardiovascular function. However, the effects of the combination of anabolic/androgenic steroid (AAS) treatment and exercise training on systolic and diastolic cardiac function are poorly understood. In the prese...

Descripción completa

Detalles Bibliográficos
Autores principales: Bocalini, Danilo S., Beutel, Abram, Bergamaschi, Cássia T., Tucci, Paulo J., Campos, Ruy R.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2014
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3922753/
https://www.ncbi.nlm.nih.gov/pubmed/24533053
http://dx.doi.org/10.1371/journal.pone.0087106
_version_ 1782303500197167104
author Bocalini, Danilo S.
Beutel, Abram
Bergamaschi, Cássia T.
Tucci, Paulo J.
Campos, Ruy R.
author_facet Bocalini, Danilo S.
Beutel, Abram
Bergamaschi, Cássia T.
Tucci, Paulo J.
Campos, Ruy R.
author_sort Bocalini, Danilo S.
collection PubMed
description Elevated concentrations of testosterone and its synthetic analogs may induce changes in cardiovascular function. However, the effects of the combination of anabolic/androgenic steroid (AAS) treatment and exercise training on systolic and diastolic cardiac function are poorly understood. In the present study, we aimed to investigate the effects of low-dose steroid treatment (stanozolol) on cardiac contractile parameters when this steroid treatment was combined with exercise training in rats and the effects of chronic steroid treatment on the Frank-Starling (length-tension curves) relationship. Male Wistar rats were randomly assigned to one of four groups: U (untrained), US (untrained and treated with stanozolol 5 mg/kg/week), T (trained, 16 m/min/1 h) and TS (trained and treated with stanozolol 5 mg/kg/week). Continuous exercise training was conducted 5 days/week for 8 consecutive weeks. The speed of the treadmill was gradually increased to a final setting of 16 m/min/1 h. Experiments were divided into two independent series: 1) central hemodynamic analysis for mean arterial blood pressure (MAP) and cardiac output (CO) measurements and 2) isolated papillary muscle preparation in Krebs solution. Stanozolol treatment significantly increased the MAP and the heart size in untrained and trained rats (U 113±2; T 106±2; US 138±8 and TS 130±7 mmHg). Furthermore, stanozolol significantly decreased developed tension and dT/dt (maximal and minimal) in U rats. However, the developed tension was completely restored by training. The Frank/Starling relationship was impaired in rats treated with stanozolol; however, again, training completely restored diastolic function. Taken together, the present data suggest that AAS treatment is able to decrease cardiac performance (systolic and diastolic functions). The combination of stanozolol and physical training improved cardiac performance, including diastolic and systolic functions, independent of changes in central hemodynamic parameters. Therefore, changes in ventricular myocyte calcium transients may play a cardioprotective role.
format Online
Article
Text
id pubmed-3922753
institution National Center for Biotechnology Information
language English
publishDate 2014
publisher Public Library of Science
record_format MEDLINE/PubMed
spelling pubmed-39227532014-02-14 Treadmill Exercise Training Prevents Myocardial Mechanical Dysfunction Induced by Androgenic-Anabolic Steroid Treatment in Rats Bocalini, Danilo S. Beutel, Abram Bergamaschi, Cássia T. Tucci, Paulo J. Campos, Ruy R. PLoS One Research Article Elevated concentrations of testosterone and its synthetic analogs may induce changes in cardiovascular function. However, the effects of the combination of anabolic/androgenic steroid (AAS) treatment and exercise training on systolic and diastolic cardiac function are poorly understood. In the present study, we aimed to investigate the effects of low-dose steroid treatment (stanozolol) on cardiac contractile parameters when this steroid treatment was combined with exercise training in rats and the effects of chronic steroid treatment on the Frank-Starling (length-tension curves) relationship. Male Wistar rats were randomly assigned to one of four groups: U (untrained), US (untrained and treated with stanozolol 5 mg/kg/week), T (trained, 16 m/min/1 h) and TS (trained and treated with stanozolol 5 mg/kg/week). Continuous exercise training was conducted 5 days/week for 8 consecutive weeks. The speed of the treadmill was gradually increased to a final setting of 16 m/min/1 h. Experiments were divided into two independent series: 1) central hemodynamic analysis for mean arterial blood pressure (MAP) and cardiac output (CO) measurements and 2) isolated papillary muscle preparation in Krebs solution. Stanozolol treatment significantly increased the MAP and the heart size in untrained and trained rats (U 113±2; T 106±2; US 138±8 and TS 130±7 mmHg). Furthermore, stanozolol significantly decreased developed tension and dT/dt (maximal and minimal) in U rats. However, the developed tension was completely restored by training. The Frank/Starling relationship was impaired in rats treated with stanozolol; however, again, training completely restored diastolic function. Taken together, the present data suggest that AAS treatment is able to decrease cardiac performance (systolic and diastolic functions). The combination of stanozolol and physical training improved cardiac performance, including diastolic and systolic functions, independent of changes in central hemodynamic parameters. Therefore, changes in ventricular myocyte calcium transients may play a cardioprotective role. Public Library of Science 2014-02-12 /pmc/articles/PMC3922753/ /pubmed/24533053 http://dx.doi.org/10.1371/journal.pone.0087106 Text en © 2014 Bocalini et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
spellingShingle Research Article
Bocalini, Danilo S.
Beutel, Abram
Bergamaschi, Cássia T.
Tucci, Paulo J.
Campos, Ruy R.
Treadmill Exercise Training Prevents Myocardial Mechanical Dysfunction Induced by Androgenic-Anabolic Steroid Treatment in Rats
title Treadmill Exercise Training Prevents Myocardial Mechanical Dysfunction Induced by Androgenic-Anabolic Steroid Treatment in Rats
title_full Treadmill Exercise Training Prevents Myocardial Mechanical Dysfunction Induced by Androgenic-Anabolic Steroid Treatment in Rats
title_fullStr Treadmill Exercise Training Prevents Myocardial Mechanical Dysfunction Induced by Androgenic-Anabolic Steroid Treatment in Rats
title_full_unstemmed Treadmill Exercise Training Prevents Myocardial Mechanical Dysfunction Induced by Androgenic-Anabolic Steroid Treatment in Rats
title_short Treadmill Exercise Training Prevents Myocardial Mechanical Dysfunction Induced by Androgenic-Anabolic Steroid Treatment in Rats
title_sort treadmill exercise training prevents myocardial mechanical dysfunction induced by androgenic-anabolic steroid treatment in rats
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3922753/
https://www.ncbi.nlm.nih.gov/pubmed/24533053
http://dx.doi.org/10.1371/journal.pone.0087106
work_keys_str_mv AT bocalinidanilos treadmillexercisetrainingpreventsmyocardialmechanicaldysfunctioninducedbyandrogenicanabolicsteroidtreatmentinrats
AT beutelabram treadmillexercisetrainingpreventsmyocardialmechanicaldysfunctioninducedbyandrogenicanabolicsteroidtreatmentinrats
AT bergamaschicassiat treadmillexercisetrainingpreventsmyocardialmechanicaldysfunctioninducedbyandrogenicanabolicsteroidtreatmentinrats
AT tuccipauloj treadmillexercisetrainingpreventsmyocardialmechanicaldysfunctioninducedbyandrogenicanabolicsteroidtreatmentinrats
AT camposruyr treadmillexercisetrainingpreventsmyocardialmechanicaldysfunctioninducedbyandrogenicanabolicsteroidtreatmentinrats