A Simple and Highly Effective Method for Slow-Freezing Human Pluripotent Stem Cells Using Dimethyl Sulfoxide, Hydroxyethyl Starch and Ethylene Glycol

Vitrification and slow-freezing methods have been used for the cryopreservation of human pluripotent stem cells (hPSCs). Vitrification requires considerable skill and post-thaw recovery is low. Furthermore, it is not suitable for cryopreservation of large numbers of hPSCs. While slow-freezing method...

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Detalles Bibliográficos
Autores principales: Imaizumi, Keitaro, Nishishita, Naoki, Muramatsu, Marie, Yamamoto, Takako, Takenaka, Chiemi, Kawamata, Shin, Kobayashi, Kenichiro, Nishikawa, Shin-ichi, Akuta, Teruo
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2014
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3922972/
https://www.ncbi.nlm.nih.gov/pubmed/24533137
http://dx.doi.org/10.1371/journal.pone.0088696

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