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Analysis of the role of PrrA, PpsR, and FnrL in intracytoplasmic membrane differentiation of Rhodobacter sphaeroides 2.4.1 using transmission electron microscopy

Oxygen dictates the catabolic “lifestyle” of Rhodobacter sphaeroides. When it is present, the bacteria are fully equipped for aerobic respiration. When it is absent, the cells outfit themselves to make use of energy-gathering options that do not require oxygen. Thus, while respiring on alternate ele...

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Autores principales: Fedotova, Yana, Zeilstra-Ryalls, Jill
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Springer Netherlands 2013
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3923116/
https://www.ncbi.nlm.nih.gov/pubmed/24146256
http://dx.doi.org/10.1007/s11120-013-9944-9
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author Fedotova, Yana
Zeilstra-Ryalls, Jill
author_facet Fedotova, Yana
Zeilstra-Ryalls, Jill
author_sort Fedotova, Yana
collection PubMed
description Oxygen dictates the catabolic “lifestyle” of Rhodobacter sphaeroides. When it is present, the bacteria are fully equipped for aerobic respiration. When it is absent, the cells outfit themselves to make use of energy-gathering options that do not require oxygen. Thus, while respiring on alternate electron acceptors in the absence of oxygen even in the dark, the cells are fully enabled for phototrophy. PrrA, PpsR, and FnrL are global regulatory proteins mediating oxygen control of gene expression in this organism. For each of these, regulon members include a subset of a cluster of genes known as the photosynthesis genes, which encode the structural proteins and enzymes catalyzing biosynthesis of the pigments of the light-harvesting and reaction center complexes. The complexes are housed in a specialized structure called the intracytoplasmic membrane (ICM). Although details are emerging as to the differentiation process leading to fully formed ICM, little is known of necessary regulatory events beyond changes in photosynthesis gene transcription. This study used transmission electron microscopy toward gaining additional insights into potential roles of PrrA, PpsR, and FnrL in the formation of ICM. The major findings were (1) the absence of either PrrA or FnrL negatively affects ICM formation, (2) the lack of ICM in the absence of PrrA is partially, but not fully reversed by removing PpsR from the cell, (3) unlike R. sphaeroides, ICM formation in Rhodobacter capsulatus does not require FnrL. New avenues these findings provide toward identifying additional genes involved in ICM formation are discussed.
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spelling pubmed-39231162014-02-24 Analysis of the role of PrrA, PpsR, and FnrL in intracytoplasmic membrane differentiation of Rhodobacter sphaeroides 2.4.1 using transmission electron microscopy Fedotova, Yana Zeilstra-Ryalls, Jill Photosynth Res Regular Paper Oxygen dictates the catabolic “lifestyle” of Rhodobacter sphaeroides. When it is present, the bacteria are fully equipped for aerobic respiration. When it is absent, the cells outfit themselves to make use of energy-gathering options that do not require oxygen. Thus, while respiring on alternate electron acceptors in the absence of oxygen even in the dark, the cells are fully enabled for phototrophy. PrrA, PpsR, and FnrL are global regulatory proteins mediating oxygen control of gene expression in this organism. For each of these, regulon members include a subset of a cluster of genes known as the photosynthesis genes, which encode the structural proteins and enzymes catalyzing biosynthesis of the pigments of the light-harvesting and reaction center complexes. The complexes are housed in a specialized structure called the intracytoplasmic membrane (ICM). Although details are emerging as to the differentiation process leading to fully formed ICM, little is known of necessary regulatory events beyond changes in photosynthesis gene transcription. This study used transmission electron microscopy toward gaining additional insights into potential roles of PrrA, PpsR, and FnrL in the formation of ICM. The major findings were (1) the absence of either PrrA or FnrL negatively affects ICM formation, (2) the lack of ICM in the absence of PrrA is partially, but not fully reversed by removing PpsR from the cell, (3) unlike R. sphaeroides, ICM formation in Rhodobacter capsulatus does not require FnrL. New avenues these findings provide toward identifying additional genes involved in ICM formation are discussed. Springer Netherlands 2013-10-22 2014 /pmc/articles/PMC3923116/ /pubmed/24146256 http://dx.doi.org/10.1007/s11120-013-9944-9 Text en © The Author(s) 2013 https://creativecommons.org/licenses/by/2.0/ Open AccessThis article is distributed under the terms of the Creative Commons Attribution License which permits any use, distribution, and reproduction in any medium, provided the original author(s) and the source are credited.
spellingShingle Regular Paper
Fedotova, Yana
Zeilstra-Ryalls, Jill
Analysis of the role of PrrA, PpsR, and FnrL in intracytoplasmic membrane differentiation of Rhodobacter sphaeroides 2.4.1 using transmission electron microscopy
title Analysis of the role of PrrA, PpsR, and FnrL in intracytoplasmic membrane differentiation of Rhodobacter sphaeroides 2.4.1 using transmission electron microscopy
title_full Analysis of the role of PrrA, PpsR, and FnrL in intracytoplasmic membrane differentiation of Rhodobacter sphaeroides 2.4.1 using transmission electron microscopy
title_fullStr Analysis of the role of PrrA, PpsR, and FnrL in intracytoplasmic membrane differentiation of Rhodobacter sphaeroides 2.4.1 using transmission electron microscopy
title_full_unstemmed Analysis of the role of PrrA, PpsR, and FnrL in intracytoplasmic membrane differentiation of Rhodobacter sphaeroides 2.4.1 using transmission electron microscopy
title_short Analysis of the role of PrrA, PpsR, and FnrL in intracytoplasmic membrane differentiation of Rhodobacter sphaeroides 2.4.1 using transmission electron microscopy
title_sort analysis of the role of prra, ppsr, and fnrl in intracytoplasmic membrane differentiation of rhodobacter sphaeroides 2.4.1 using transmission electron microscopy
topic Regular Paper
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3923116/
https://www.ncbi.nlm.nih.gov/pubmed/24146256
http://dx.doi.org/10.1007/s11120-013-9944-9
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