Cargando…

Hazards of Secondary Bromadiolone Intoxications Evaluated using High-performance Liquid Chromatography with Electrochemical Detection

This study reported on the possibility of intoxications of non-target wild animals associated with use of bromadiolone as the active component of rodenticides with anticoagulation effects. A laboratory test was done with earthworms were exposed to bromadiolone-containing granules under the condition...

Descripción completa

Detalles Bibliográficos
Autores principales: Krizkova, Sona, Beklova, Miroslava, Pikula, Jiri, Adam, Vojtech, Horna, Ales, Kizek, René
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Molecular Diversity Preservation International (MDPI) 2007
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3923180/
_version_ 1782303577970049024
author Krizkova, Sona
Beklova, Miroslava
Pikula, Jiri
Adam, Vojtech
Horna, Ales
Kizek, René
author_facet Krizkova, Sona
Beklova, Miroslava
Pikula, Jiri
Adam, Vojtech
Horna, Ales
Kizek, René
author_sort Krizkova, Sona
collection PubMed
description This study reported on the possibility of intoxications of non-target wild animals associated with use of bromadiolone as the active component of rodenticides with anticoagulation effects. A laboratory test was done with earthworms were exposed to bromadiolone-containing granules under the conditions specified in the modified OECD 207 guideline. No mortality of earthworms was observed during the fourteen days long exposure. When the earthworms from the above test became a part of the diet of common voles in the following experiment, no mortality of consumers was observed too. However, electrochemical analysis revealed higher levels of bromadiolone in tissues from earthworms as well as common voles compared to control animals. There were determined comparable levels of bromadiolone in the liver tissue of common voles after primary (2.34±0.10 μg/g) and secondary (2.20±0.53 μg/g) intoxication. Therefore, the risk of secondary intoxication of small mammalian species feeding on bromadiolone-containing earthworms is the same as of primary intoxication through baited granules. Bromadiolone bio-accumulation in the food chain was monitored using the newly developed analytical procedure based on the use of a liquid chromatography coupled with electrochemical detector (HPLC-ED). The HPLC-ED method allowed to determine the levels of bromadiolone in biological samples and is therefore suitable for examining the environmental hazards of this substance.
format Online
Article
Text
id pubmed-3923180
institution National Center for Biotechnology Information
language English
publishDate 2007
publisher Molecular Diversity Preservation International (MDPI)
record_format MEDLINE/PubMed
spelling pubmed-39231802014-02-13 Hazards of Secondary Bromadiolone Intoxications Evaluated using High-performance Liquid Chromatography with Electrochemical Detection Krizkova, Sona Beklova, Miroslava Pikula, Jiri Adam, Vojtech Horna, Ales Kizek, René Sensors (Basel) Full Research Paper This study reported on the possibility of intoxications of non-target wild animals associated with use of bromadiolone as the active component of rodenticides with anticoagulation effects. A laboratory test was done with earthworms were exposed to bromadiolone-containing granules under the conditions specified in the modified OECD 207 guideline. No mortality of earthworms was observed during the fourteen days long exposure. When the earthworms from the above test became a part of the diet of common voles in the following experiment, no mortality of consumers was observed too. However, electrochemical analysis revealed higher levels of bromadiolone in tissues from earthworms as well as common voles compared to control animals. There were determined comparable levels of bromadiolone in the liver tissue of common voles after primary (2.34±0.10 μg/g) and secondary (2.20±0.53 μg/g) intoxication. Therefore, the risk of secondary intoxication of small mammalian species feeding on bromadiolone-containing earthworms is the same as of primary intoxication through baited granules. Bromadiolone bio-accumulation in the food chain was monitored using the newly developed analytical procedure based on the use of a liquid chromatography coupled with electrochemical detector (HPLC-ED). The HPLC-ED method allowed to determine the levels of bromadiolone in biological samples and is therefore suitable for examining the environmental hazards of this substance. Molecular Diversity Preservation International (MDPI) 2007-07-20 /pmc/articles/PMC3923180/ Text en © 2007 by MDPI (http://www.mdpi.org). Reproduction is permitted for noncommercial purposes.
spellingShingle Full Research Paper
Krizkova, Sona
Beklova, Miroslava
Pikula, Jiri
Adam, Vojtech
Horna, Ales
Kizek, René
Hazards of Secondary Bromadiolone Intoxications Evaluated using High-performance Liquid Chromatography with Electrochemical Detection
title Hazards of Secondary Bromadiolone Intoxications Evaluated using High-performance Liquid Chromatography with Electrochemical Detection
title_full Hazards of Secondary Bromadiolone Intoxications Evaluated using High-performance Liquid Chromatography with Electrochemical Detection
title_fullStr Hazards of Secondary Bromadiolone Intoxications Evaluated using High-performance Liquid Chromatography with Electrochemical Detection
title_full_unstemmed Hazards of Secondary Bromadiolone Intoxications Evaluated using High-performance Liquid Chromatography with Electrochemical Detection
title_short Hazards of Secondary Bromadiolone Intoxications Evaluated using High-performance Liquid Chromatography with Electrochemical Detection
title_sort hazards of secondary bromadiolone intoxications evaluated using high-performance liquid chromatography with electrochemical detection
topic Full Research Paper
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3923180/
work_keys_str_mv AT krizkovasona hazardsofsecondarybromadioloneintoxicationsevaluatedusinghighperformanceliquidchromatographywithelectrochemicaldetection
AT beklovamiroslava hazardsofsecondarybromadioloneintoxicationsevaluatedusinghighperformanceliquidchromatographywithelectrochemicaldetection
AT pikulajiri hazardsofsecondarybromadioloneintoxicationsevaluatedusinghighperformanceliquidchromatographywithelectrochemicaldetection
AT adamvojtech hazardsofsecondarybromadioloneintoxicationsevaluatedusinghighperformanceliquidchromatographywithelectrochemicaldetection
AT hornaales hazardsofsecondarybromadioloneintoxicationsevaluatedusinghighperformanceliquidchromatographywithelectrochemicaldetection
AT kizekrene hazardsofsecondarybromadioloneintoxicationsevaluatedusinghighperformanceliquidchromatographywithelectrochemicaldetection