Identification of Specific Cell-Surface Markers of Adipose-Derived Stem Cells from Subcutaneous and Visceral Fat Depots

Adipose-derived stem/stromal cells (ASCs) from the anatomically distinct subcutaneous and visceral depots of white adipose tissue (WAT) differ in their inherent properties. However, little is known about the molecular identity and definitive markers of ASCs from these depots. In this study, ASCs fro...

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Autores principales: Ong, Wee Kiat, Tan, Chuen Seng, Chan, Kai Li, Goesantoso, Grace Gandi, Chan, Xin Hui Derryn, Chan, Edmund, Yin, Jocelyn, Yeo, Chia Rou, Khoo, Chin Meng, So, Jimmy Bok Yan, Shabbir, Asim, Toh, Sue-Anne, Han, Weiping, Sugii, Shigeki
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2014
Materias:
Report
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3923222/
https://www.ncbi.nlm.nih.gov/pubmed/24527391
http://dx.doi.org/10.1016/j.stemcr.2014.01.002
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author Ong, Wee Kiat
Tan, Chuen Seng
Chan, Kai Li
Goesantoso, Grace Gandi
Chan, Xin Hui Derryn
Chan, Edmund
Yin, Jocelyn
Yeo, Chia Rou
Khoo, Chin Meng
So, Jimmy Bok Yan
Shabbir, Asim
Toh, Sue-Anne
Han, Weiping
Sugii, Shigeki
author_facet Ong, Wee Kiat
Tan, Chuen Seng
Chan, Kai Li
Goesantoso, Grace Gandi
Chan, Xin Hui Derryn
Chan, Edmund
Yin, Jocelyn
Yeo, Chia Rou
Khoo, Chin Meng
So, Jimmy Bok Yan
Shabbir, Asim
Toh, Sue-Anne
Han, Weiping
Sugii, Shigeki
author_sort Ong, Wee Kiat
collection PubMed
description Adipose-derived stem/stromal cells (ASCs) from the anatomically distinct subcutaneous and visceral depots of white adipose tissue (WAT) differ in their inherent properties. However, little is known about the molecular identity and definitive markers of ASCs from these depots. In this study, ASCs from subcutaneous fat (SC-ASCs) and visceral fat (VS-ASCs) of omental region were isolated and studied. High-content image screening of over 240 cell-surface markers identified several potential depot-specific markers of ASCs. Subsequent studies revealed consistent predominant expression of CD10 in SC-ASCs and CD200 in VS-ASCs across 12 human subjects and in mice. CD10-high-expressing cells sorted from SC-ASCs differentiated better than their CD10-low-expressing counterparts, whereas CD200-low VS-ASCs differentiated better than CD200-high VS-ASCs. The expression of CD10 and CD200 is thus depot-dependent and associates with adipogenic capacities. These markers will offer a valuable tool for tracking and screening of depot-specific stem cell populations.
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spelling pubmed-39232222014-02-13 Identification of Specific Cell-Surface Markers of Adipose-Derived Stem Cells from Subcutaneous and Visceral Fat Depots Ong, Wee Kiat Tan, Chuen Seng Chan, Kai Li Goesantoso, Grace Gandi Chan, Xin Hui Derryn Chan, Edmund Yin, Jocelyn Yeo, Chia Rou Khoo, Chin Meng So, Jimmy Bok Yan Shabbir, Asim Toh, Sue-Anne Han, Weiping Sugii, Shigeki Stem Cell Reports Report Adipose-derived stem/stromal cells (ASCs) from the anatomically distinct subcutaneous and visceral depots of white adipose tissue (WAT) differ in their inherent properties. However, little is known about the molecular identity and definitive markers of ASCs from these depots. In this study, ASCs from subcutaneous fat (SC-ASCs) and visceral fat (VS-ASCs) of omental region were isolated and studied. High-content image screening of over 240 cell-surface markers identified several potential depot-specific markers of ASCs. Subsequent studies revealed consistent predominant expression of CD10 in SC-ASCs and CD200 in VS-ASCs across 12 human subjects and in mice. CD10-high-expressing cells sorted from SC-ASCs differentiated better than their CD10-low-expressing counterparts, whereas CD200-low VS-ASCs differentiated better than CD200-high VS-ASCs. The expression of CD10 and CD200 is thus depot-dependent and associates with adipogenic capacities. These markers will offer a valuable tool for tracking and screening of depot-specific stem cell populations. Elsevier 2014-02-06 /pmc/articles/PMC3923222/ /pubmed/24527391 http://dx.doi.org/10.1016/j.stemcr.2014.01.002 Text en © 2014 The Authors http://creativecommons.org/licenses/by-nc-nd/3.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution-NonCommercial-No Derivative Works License, which permits non-commercial use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Report
Ong, Wee Kiat
Tan, Chuen Seng
Chan, Kai Li
Goesantoso, Grace Gandi
Chan, Xin Hui Derryn
Chan, Edmund
Yin, Jocelyn
Yeo, Chia Rou
Khoo, Chin Meng
So, Jimmy Bok Yan
Shabbir, Asim
Toh, Sue-Anne
Han, Weiping
Sugii, Shigeki
Identification of Specific Cell-Surface Markers of Adipose-Derived Stem Cells from Subcutaneous and Visceral Fat Depots
title Identification of Specific Cell-Surface Markers of Adipose-Derived Stem Cells from Subcutaneous and Visceral Fat Depots
title_full Identification of Specific Cell-Surface Markers of Adipose-Derived Stem Cells from Subcutaneous and Visceral Fat Depots
title_fullStr Identification of Specific Cell-Surface Markers of Adipose-Derived Stem Cells from Subcutaneous and Visceral Fat Depots
title_full_unstemmed Identification of Specific Cell-Surface Markers of Adipose-Derived Stem Cells from Subcutaneous and Visceral Fat Depots
title_short Identification of Specific Cell-Surface Markers of Adipose-Derived Stem Cells from Subcutaneous and Visceral Fat Depots
title_sort identification of specific cell-surface markers of adipose-derived stem cells from subcutaneous and visceral fat depots
topic Report
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3923222/
https://www.ncbi.nlm.nih.gov/pubmed/24527391
http://dx.doi.org/10.1016/j.stemcr.2014.01.002
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