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Derivation and Maintenance of Murine Trophoblast Stem Cells under Defined Conditions
Trophoblast stem cells (TSCs) are in vitro equivalents to the precursor cells of the placenta. TSCs are cultured in serum-rich medium with fibroblast growth factor 4, heparin, and embryonic-fibroblast-conditioned medium. Here, we developed a simple medium consisting of ten chemically defined ingredi...
Autores principales: | , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Elsevier
2014
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3923226/ https://www.ncbi.nlm.nih.gov/pubmed/24527396 http://dx.doi.org/10.1016/j.stemcr.2013.12.013 |
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author | Kubaczka, Caroline Senner, Claire Araúzo-Bravo, Marcos J. Sharma, Neha Kuckenberg, Peter Becker, Astrid Zimmer, Andreas Brüstle, Oliver Peitz, Michael Hemberger, Myriam Schorle, Hubert |
author_facet | Kubaczka, Caroline Senner, Claire Araúzo-Bravo, Marcos J. Sharma, Neha Kuckenberg, Peter Becker, Astrid Zimmer, Andreas Brüstle, Oliver Peitz, Michael Hemberger, Myriam Schorle, Hubert |
author_sort | Kubaczka, Caroline |
collection | PubMed |
description | Trophoblast stem cells (TSCs) are in vitro equivalents to the precursor cells of the placenta. TSCs are cultured in serum-rich medium with fibroblast growth factor 4, heparin, and embryonic-fibroblast-conditioned medium. Here, we developed a simple medium consisting of ten chemically defined ingredients for culture of TSCs on Matrigel or synthetic substrates, named TX medium. Gene expression and DNA methylation profiling demonstrated the faithful propagation of expression profiles and epigenomic characteristics of TSCs cultured in TX. Further, TX medium supported the de novo derivation of TSC lines. Finally, TSCs cultured in TX differentiate into all derivatives of the trophectodermal lineage in vitro, give rise to hemorrhagic lesions in nude mice, and chimerize the placenta, indicating that they retained all hallmarks of TSCs. TX media formulation no longer requires fetal bovine serum and conditioned medium, which facilitates and standardizes the culture of this extraembryonic lineage. |
format | Online Article Text |
id | pubmed-3923226 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2014 |
publisher | Elsevier |
record_format | MEDLINE/PubMed |
spelling | pubmed-39232262014-02-13 Derivation and Maintenance of Murine Trophoblast Stem Cells under Defined Conditions Kubaczka, Caroline Senner, Claire Araúzo-Bravo, Marcos J. Sharma, Neha Kuckenberg, Peter Becker, Astrid Zimmer, Andreas Brüstle, Oliver Peitz, Michael Hemberger, Myriam Schorle, Hubert Stem Cell Reports Article Trophoblast stem cells (TSCs) are in vitro equivalents to the precursor cells of the placenta. TSCs are cultured in serum-rich medium with fibroblast growth factor 4, heparin, and embryonic-fibroblast-conditioned medium. Here, we developed a simple medium consisting of ten chemically defined ingredients for culture of TSCs on Matrigel or synthetic substrates, named TX medium. Gene expression and DNA methylation profiling demonstrated the faithful propagation of expression profiles and epigenomic characteristics of TSCs cultured in TX. Further, TX medium supported the de novo derivation of TSC lines. Finally, TSCs cultured in TX differentiate into all derivatives of the trophectodermal lineage in vitro, give rise to hemorrhagic lesions in nude mice, and chimerize the placenta, indicating that they retained all hallmarks of TSCs. TX media formulation no longer requires fetal bovine serum and conditioned medium, which facilitates and standardizes the culture of this extraembryonic lineage. Elsevier 2014-01-30 /pmc/articles/PMC3923226/ /pubmed/24527396 http://dx.doi.org/10.1016/j.stemcr.2013.12.013 Text en © 2014 The Authors http://creativecommons.org/licenses/by-nc-nd/3.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution-NonCommercial-No Derivative Works License, which permits non-commercial use, distribution, and reproduction in any medium, provided the original author and source are credited. |
spellingShingle | Article Kubaczka, Caroline Senner, Claire Araúzo-Bravo, Marcos J. Sharma, Neha Kuckenberg, Peter Becker, Astrid Zimmer, Andreas Brüstle, Oliver Peitz, Michael Hemberger, Myriam Schorle, Hubert Derivation and Maintenance of Murine Trophoblast Stem Cells under Defined Conditions |
title | Derivation and Maintenance of Murine Trophoblast Stem Cells under Defined Conditions |
title_full | Derivation and Maintenance of Murine Trophoblast Stem Cells under Defined Conditions |
title_fullStr | Derivation and Maintenance of Murine Trophoblast Stem Cells under Defined Conditions |
title_full_unstemmed | Derivation and Maintenance of Murine Trophoblast Stem Cells under Defined Conditions |
title_short | Derivation and Maintenance of Murine Trophoblast Stem Cells under Defined Conditions |
title_sort | derivation and maintenance of murine trophoblast stem cells under defined conditions |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3923226/ https://www.ncbi.nlm.nih.gov/pubmed/24527396 http://dx.doi.org/10.1016/j.stemcr.2013.12.013 |
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