Cargando…
MiR-200a inhibits epithelial-mesenchymal transition of pancreatic cancer stem cell
BACKGROUND: Pancreatic cancer is one of the most aggressive cancers, and the aggressiveness of pancreatic cancer is in part due to its intrinsic and extrinsic drug resistance characteristics, which are also associated with the acquisition of epithelial-to-mesenchymal transition (EMT). Increasing evi...
Autores principales: | , , , , , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
BioMed Central
2014
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3923443/ https://www.ncbi.nlm.nih.gov/pubmed/24521357 http://dx.doi.org/10.1186/1471-2407-14-85 |
_version_ | 1782303619683450880 |
---|---|
author | Lu, Yuhua Lu, Jingjing Li, Xiaohong Zhu, Hui Fan, Xiangjun Zhu, Shajun Wang, Yao Guo, Qingsong Wang, Lei Huang, Yan Zhu, Mingyan Wang, Zhiwei |
author_facet | Lu, Yuhua Lu, Jingjing Li, Xiaohong Zhu, Hui Fan, Xiangjun Zhu, Shajun Wang, Yao Guo, Qingsong Wang, Lei Huang, Yan Zhu, Mingyan Wang, Zhiwei |
author_sort | Lu, Yuhua |
collection | PubMed |
description | BACKGROUND: Pancreatic cancer is one of the most aggressive cancers, and the aggressiveness of pancreatic cancer is in part due to its intrinsic and extrinsic drug resistance characteristics, which are also associated with the acquisition of epithelial-to-mesenchymal transition (EMT). Increasing evidence suggests that EMT-type cells share many biological characteristics with cancer stem-like cells. And miR-200 has been identified as a powerful regulator of EMT. METHODS: Cancer Stem Cells (CSCs) of human pancreatic cancer cell line PANC-1 were processed for CD24, CD44 and ESA multi-colorstaining, and sorted out on a BD FACS Aria II machine. RT-qPCR was performed using the miScript PCR Kit to assay the expression of miR-200 family. In order to find the role of miR-200a in the process of EMT, miR-200a mimic was transfected to CSCs. RESULTS: Pancreatic cancer cells with EMT phenotype displayed stem-like cell features characterized by the expression of cell surface markers CD24, CD44 and epithelial-specific antigen (ESA), which was associated with decreased expression of miR-200a. Moreover, overexpression of miR-200a was resulted in down-regulation of N-cadherin, ZEB1 and vimentin, but up-regulation of E-cadherin. In addition, miR-200a overexpression inhibited cell migration and invasion in CSCs. CONCLUSION: In our study, we found that miR-200a played an important role in linking the characteristics of cancer stem-like cells with EMT-like cell signatures in pancreatic cancer. Selective elimination of cancer stem-like cells by reversing the EMT phenotype to mesenchymal-to-epithelial transition (MET) phenotype using novel agents would be useful for prevention and/or treatment of pancreatic cancer. |
format | Online Article Text |
id | pubmed-3923443 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2014 |
publisher | BioMed Central |
record_format | MEDLINE/PubMed |
spelling | pubmed-39234432014-02-14 MiR-200a inhibits epithelial-mesenchymal transition of pancreatic cancer stem cell Lu, Yuhua Lu, Jingjing Li, Xiaohong Zhu, Hui Fan, Xiangjun Zhu, Shajun Wang, Yao Guo, Qingsong Wang, Lei Huang, Yan Zhu, Mingyan Wang, Zhiwei BMC Cancer Research Article BACKGROUND: Pancreatic cancer is one of the most aggressive cancers, and the aggressiveness of pancreatic cancer is in part due to its intrinsic and extrinsic drug resistance characteristics, which are also associated with the acquisition of epithelial-to-mesenchymal transition (EMT). Increasing evidence suggests that EMT-type cells share many biological characteristics with cancer stem-like cells. And miR-200 has been identified as a powerful regulator of EMT. METHODS: Cancer Stem Cells (CSCs) of human pancreatic cancer cell line PANC-1 were processed for CD24, CD44 and ESA multi-colorstaining, and sorted out on a BD FACS Aria II machine. RT-qPCR was performed using the miScript PCR Kit to assay the expression of miR-200 family. In order to find the role of miR-200a in the process of EMT, miR-200a mimic was transfected to CSCs. RESULTS: Pancreatic cancer cells with EMT phenotype displayed stem-like cell features characterized by the expression of cell surface markers CD24, CD44 and epithelial-specific antigen (ESA), which was associated with decreased expression of miR-200a. Moreover, overexpression of miR-200a was resulted in down-regulation of N-cadherin, ZEB1 and vimentin, but up-regulation of E-cadherin. In addition, miR-200a overexpression inhibited cell migration and invasion in CSCs. CONCLUSION: In our study, we found that miR-200a played an important role in linking the characteristics of cancer stem-like cells with EMT-like cell signatures in pancreatic cancer. Selective elimination of cancer stem-like cells by reversing the EMT phenotype to mesenchymal-to-epithelial transition (MET) phenotype using novel agents would be useful for prevention and/or treatment of pancreatic cancer. BioMed Central 2014-02-12 /pmc/articles/PMC3923443/ /pubmed/24521357 http://dx.doi.org/10.1186/1471-2407-14-85 Text en Copyright © 2014 Lu et al.; licensee BioMed Central Ltd. http://creativecommons.org/licenses/by/2.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. |
spellingShingle | Research Article Lu, Yuhua Lu, Jingjing Li, Xiaohong Zhu, Hui Fan, Xiangjun Zhu, Shajun Wang, Yao Guo, Qingsong Wang, Lei Huang, Yan Zhu, Mingyan Wang, Zhiwei MiR-200a inhibits epithelial-mesenchymal transition of pancreatic cancer stem cell |
title | MiR-200a inhibits epithelial-mesenchymal transition of pancreatic cancer stem cell |
title_full | MiR-200a inhibits epithelial-mesenchymal transition of pancreatic cancer stem cell |
title_fullStr | MiR-200a inhibits epithelial-mesenchymal transition of pancreatic cancer stem cell |
title_full_unstemmed | MiR-200a inhibits epithelial-mesenchymal transition of pancreatic cancer stem cell |
title_short | MiR-200a inhibits epithelial-mesenchymal transition of pancreatic cancer stem cell |
title_sort | mir-200a inhibits epithelial-mesenchymal transition of pancreatic cancer stem cell |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3923443/ https://www.ncbi.nlm.nih.gov/pubmed/24521357 http://dx.doi.org/10.1186/1471-2407-14-85 |
work_keys_str_mv | AT luyuhua mir200ainhibitsepithelialmesenchymaltransitionofpancreaticcancerstemcell AT lujingjing mir200ainhibitsepithelialmesenchymaltransitionofpancreaticcancerstemcell AT lixiaohong mir200ainhibitsepithelialmesenchymaltransitionofpancreaticcancerstemcell AT zhuhui mir200ainhibitsepithelialmesenchymaltransitionofpancreaticcancerstemcell AT fanxiangjun mir200ainhibitsepithelialmesenchymaltransitionofpancreaticcancerstemcell AT zhushajun mir200ainhibitsepithelialmesenchymaltransitionofpancreaticcancerstemcell AT wangyao mir200ainhibitsepithelialmesenchymaltransitionofpancreaticcancerstemcell AT guoqingsong mir200ainhibitsepithelialmesenchymaltransitionofpancreaticcancerstemcell AT wanglei mir200ainhibitsepithelialmesenchymaltransitionofpancreaticcancerstemcell AT huangyan mir200ainhibitsepithelialmesenchymaltransitionofpancreaticcancerstemcell AT zhumingyan mir200ainhibitsepithelialmesenchymaltransitionofpancreaticcancerstemcell AT wangzhiwei mir200ainhibitsepithelialmesenchymaltransitionofpancreaticcancerstemcell |