Cargando…
abFASP-MS: Affinity-Based Filter-Aided Sample Preparation Mass Spectrometry for Quantitative Analysis of Chemically Labeled Protein Complexes
[Image: see text] Affinity purification coupled to 1-D gel-free liquid chromatography mass spectrometry (LC–MS) is a well-established and widespread approach for the analyses of noncovalently interacting protein complexes. In this study, two proteins conjugated to a streptavidin-binding peptide and...
Autores principales: | Huber, Marie L., Sacco, Roberto, Parapatics, Katja, Skucha, Anna, Khamina, Kseniya, Müller, André C., Rudashevskaya, Elena L., Bennett, Keiryn L. |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
American Chemical
Society
2014
|
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3923452/ https://www.ncbi.nlm.nih.gov/pubmed/24400740 http://dx.doi.org/10.1021/pr4009892 |
Ejemplares similares
-
Enhanced FASP (eFASP) to Increase
Proteome Coverage
and Sample Recovery for Quantitative Proteomic Experiments
por: Erde, Jonathan, et al.
Publicado: (2014) -
Affinity Purification Strategies for Proteomic Analysis
of Transcription Factor Complexes
por: Giambruno, Roberto, et al.
Publicado: (2013) -
A modified FASP protocol for high-throughput preparation of protein samples for mass spectrometry
por: Potriquet, Jeremy, et al.
Publicado: (2017) -
Well-Plate μFASP
for Proteomic Analysis of Single
Pancreatic Islets
por: Sandbaumhüter, Friederike A., et al.
Publicado: (2022) -
Thiol-free reducing agents in electrophoretic separations and FASP proteolytic digestions for the analysis of metal-binding proteins
por: Moraleja, I., et al.
Publicado: (2014)