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Soft matrix is a natural stimulator for cellular invasiveness
Directional mesenchymal cell invasion in vivo is understood to be a stimulated event and to be regulated by cytokines, chemokines, and types of extracellular matrix (ECM). Instead, by focusing on the cellular response to ECM stiffness, we found that soft ECM (low stiffness) itself is sufficient to p...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
The American Society for Cell Biology
2014
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3923638/ https://www.ncbi.nlm.nih.gov/pubmed/24336521 http://dx.doi.org/10.1091/mbc.E13-05-0260 |
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author | Gu, Zhizhan Liu, Fei Tonkova, Elina A. Lee, Soo Young Tschumperlin, Daniel J. Brenner, Michael B. |
author_facet | Gu, Zhizhan Liu, Fei Tonkova, Elina A. Lee, Soo Young Tschumperlin, Daniel J. Brenner, Michael B. |
author_sort | Gu, Zhizhan |
collection | PubMed |
description | Directional mesenchymal cell invasion in vivo is understood to be a stimulated event and to be regulated by cytokines, chemokines, and types of extracellular matrix (ECM). Instead, by focusing on the cellular response to ECM stiffness, we found that soft ECM (low stiffness) itself is sufficient to prevent stable cell-to-cell adherens junction formation, up-regulate matrix metalloproteinase (MMP) secretion, promote MMP activity, and induce invadosome-like protrusion (ILP) formation. Consistently, similar ILP formation was also detected in a three-dimensional directional invasion assay in soft matrix. Primary human fibroblasts spontaneously form ILPs in a very narrow range of ECM stiffness (0.1–0.4 kPa), and such ILP formation is Src family kinase dependent. In contrast, spontaneous ILP formation in malignant cancer cells and fibrosarcoma cells occurs across a much wider range of ECM stiffness, and these tumor cell ILPs are also more prominent at lower stiffness. These findings suggest that ECM softness is a natural stimulator for cellular invasiveness. |
format | Online Article Text |
id | pubmed-3923638 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2014 |
publisher | The American Society for Cell Biology |
record_format | MEDLINE/PubMed |
spelling | pubmed-39236382014-04-30 Soft matrix is a natural stimulator for cellular invasiveness Gu, Zhizhan Liu, Fei Tonkova, Elina A. Lee, Soo Young Tschumperlin, Daniel J. Brenner, Michael B. Mol Biol Cell Articles Directional mesenchymal cell invasion in vivo is understood to be a stimulated event and to be regulated by cytokines, chemokines, and types of extracellular matrix (ECM). Instead, by focusing on the cellular response to ECM stiffness, we found that soft ECM (low stiffness) itself is sufficient to prevent stable cell-to-cell adherens junction formation, up-regulate matrix metalloproteinase (MMP) secretion, promote MMP activity, and induce invadosome-like protrusion (ILP) formation. Consistently, similar ILP formation was also detected in a three-dimensional directional invasion assay in soft matrix. Primary human fibroblasts spontaneously form ILPs in a very narrow range of ECM stiffness (0.1–0.4 kPa), and such ILP formation is Src family kinase dependent. In contrast, spontaneous ILP formation in malignant cancer cells and fibrosarcoma cells occurs across a much wider range of ECM stiffness, and these tumor cell ILPs are also more prominent at lower stiffness. These findings suggest that ECM softness is a natural stimulator for cellular invasiveness. The American Society for Cell Biology 2014-02-15 /pmc/articles/PMC3923638/ /pubmed/24336521 http://dx.doi.org/10.1091/mbc.E13-05-0260 Text en © 2014 Gu et al. This article is distributed by The American Society for Cell Biology under license from the author(s). Two months after publication it is available to the public under an Attribution–Noncommercial–Share Alike 3.0 Unported Creative Commons License (http://creativecommons.org/licenses/by-nc-sa/3.0). “ASCB®,” “The American Society for Cell Biology®,” and “Molecular Biology of the Cell®” are registered trademarks of The American Society of Cell Biology. |
spellingShingle | Articles Gu, Zhizhan Liu, Fei Tonkova, Elina A. Lee, Soo Young Tschumperlin, Daniel J. Brenner, Michael B. Soft matrix is a natural stimulator for cellular invasiveness |
title | Soft matrix is a natural stimulator for cellular invasiveness |
title_full | Soft matrix is a natural stimulator for cellular invasiveness |
title_fullStr | Soft matrix is a natural stimulator for cellular invasiveness |
title_full_unstemmed | Soft matrix is a natural stimulator for cellular invasiveness |
title_short | Soft matrix is a natural stimulator for cellular invasiveness |
title_sort | soft matrix is a natural stimulator for cellular invasiveness |
topic | Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3923638/ https://www.ncbi.nlm.nih.gov/pubmed/24336521 http://dx.doi.org/10.1091/mbc.E13-05-0260 |
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