A Modular Plasmid Assembly Kit for Multigene Expression, Gene Silencing and Silencing Rescue in Plants

The Golden Gate (GG) modular assembly approach offers a standardized, inexpensive and reliable way to ligate multiple DNA fragments in a pre-defined order in a single-tube reaction. We developed a GG based toolkit for the flexible construction of binary plasmids for transgene expression in plants. S...

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Detalles Bibliográficos
Autores principales: Binder, Andreas, Lambert, Jayne, Morbitzer, Robert, Popp, Claudia, Ott, Thomas, Lahaye, Thomas, Parniske, Martin
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2014
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3923767/
https://www.ncbi.nlm.nih.gov/pubmed/24551083
http://dx.doi.org/10.1371/journal.pone.0088218

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3923767/
https://www.ncbi.nlm.nih.gov/pubmed/24551083
http://dx.doi.org/10.1371/journal.pone.0088218

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