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An automated maze task for assessing hippocampus-sensitive memory in mice()
Memory deficits associated with hippocampal dysfunction are a key feature of a number of neurodegenerative and psychiatric disorders. The discrete-trial rewarded alternation T-maze task is highly sensitive to hippocampal dysfunction. Normal mice have spontaneously high levels of alternation, whereas...
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Elsevier/North-Holland Biomedical Press
2014
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3923974/ https://www.ncbi.nlm.nih.gov/pubmed/24333574 http://dx.doi.org/10.1016/j.bbr.2013.12.009 |
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author | Pioli, Elsa Y. Gaskill, Brianna N. Gilmour, Gary Tricklebank, Mark D. Dix, Sophie L. Bannerman, David Garner, Joseph P. |
author_facet | Pioli, Elsa Y. Gaskill, Brianna N. Gilmour, Gary Tricklebank, Mark D. Dix, Sophie L. Bannerman, David Garner, Joseph P. |
author_sort | Pioli, Elsa Y. |
collection | PubMed |
description | Memory deficits associated with hippocampal dysfunction are a key feature of a number of neurodegenerative and psychiatric disorders. The discrete-trial rewarded alternation T-maze task is highly sensitive to hippocampal dysfunction. Normal mice have spontaneously high levels of alternation, whereas hippocampal-lesioned mice are dramatically impaired. However, this is a hand-run task and handling has been shown to impact crucially on behavioural responses, as well as being labour-intensive and therefore unsuitable for high-throughput studies. To overcome this, a fully automated maze was designed. The maze was attached to the mouse's home cage and the subject earned all of its food by running through the maze. In this study the hippocampal dependence of rewarded alternation in the automated maze was assessed. Bilateral hippocampal-lesioned mice were assessed in the standard, hand-run, discrete-trial rewarded alternation paradigm and in the automated paradigm, according to a cross-over design. A similarly robust lesion effect on alternation performance was found in both mazes, confirming the sensitivity of the automated maze to hippocampal lesions. Moreover, the performance of the animals in the automated maze was not affected by their handling history whereas performance in the hand-run maze was affected by prior testing history. By having more stable performance and by decreasing human contact the automated maze may offer opportunities to reduce extraneous experimental variation and therefore increase the reproducibility within and/or between laboratories. Furthermore, automation potentially allows for greater experimental throughput and hence suitability for use in assessment of cognitive function in drug discovery. |
format | Online Article Text |
id | pubmed-3923974 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2014 |
publisher | Elsevier/North-Holland Biomedical Press |
record_format | MEDLINE/PubMed |
spelling | pubmed-39239742014-03-15 An automated maze task for assessing hippocampus-sensitive memory in mice() Pioli, Elsa Y. Gaskill, Brianna N. Gilmour, Gary Tricklebank, Mark D. Dix, Sophie L. Bannerman, David Garner, Joseph P. Behav Brain Res Research Report Memory deficits associated with hippocampal dysfunction are a key feature of a number of neurodegenerative and psychiatric disorders. The discrete-trial rewarded alternation T-maze task is highly sensitive to hippocampal dysfunction. Normal mice have spontaneously high levels of alternation, whereas hippocampal-lesioned mice are dramatically impaired. However, this is a hand-run task and handling has been shown to impact crucially on behavioural responses, as well as being labour-intensive and therefore unsuitable for high-throughput studies. To overcome this, a fully automated maze was designed. The maze was attached to the mouse's home cage and the subject earned all of its food by running through the maze. In this study the hippocampal dependence of rewarded alternation in the automated maze was assessed. Bilateral hippocampal-lesioned mice were assessed in the standard, hand-run, discrete-trial rewarded alternation paradigm and in the automated paradigm, according to a cross-over design. A similarly robust lesion effect on alternation performance was found in both mazes, confirming the sensitivity of the automated maze to hippocampal lesions. Moreover, the performance of the animals in the automated maze was not affected by their handling history whereas performance in the hand-run maze was affected by prior testing history. By having more stable performance and by decreasing human contact the automated maze may offer opportunities to reduce extraneous experimental variation and therefore increase the reproducibility within and/or between laboratories. Furthermore, automation potentially allows for greater experimental throughput and hence suitability for use in assessment of cognitive function in drug discovery. Elsevier/North-Holland Biomedical Press 2014-03-15 /pmc/articles/PMC3923974/ /pubmed/24333574 http://dx.doi.org/10.1016/j.bbr.2013.12.009 Text en © 2013 The Authors https://creativecommons.org/licenses/by-nc-nd/3.0/This is an open access article under the CC BY NC ND license (https://creativecommons.org/licenses/by-nc-nd/3.0/). |
spellingShingle | Research Report Pioli, Elsa Y. Gaskill, Brianna N. Gilmour, Gary Tricklebank, Mark D. Dix, Sophie L. Bannerman, David Garner, Joseph P. An automated maze task for assessing hippocampus-sensitive memory in mice() |
title | An automated maze task for assessing hippocampus-sensitive memory in mice() |
title_full | An automated maze task for assessing hippocampus-sensitive memory in mice() |
title_fullStr | An automated maze task for assessing hippocampus-sensitive memory in mice() |
title_full_unstemmed | An automated maze task for assessing hippocampus-sensitive memory in mice() |
title_short | An automated maze task for assessing hippocampus-sensitive memory in mice() |
title_sort | automated maze task for assessing hippocampus-sensitive memory in mice() |
topic | Research Report |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3923974/ https://www.ncbi.nlm.nih.gov/pubmed/24333574 http://dx.doi.org/10.1016/j.bbr.2013.12.009 |
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