5′-Deoxy-5′-Hydrazinylguanosine as an Initiator of T7 RNA Polymerase-Catalyzed Transcriptions for the Preparation of Labeling-Ready RNAS

5′-deoxy-5′-hydrazinylguanosine was incorporated into the 5′-termini of RNA transcripts using T7 RNA polymerase. Transcriptions provided 5′-hydrazinyl-RNA that was readily labeled and purified. The use of fluorophore-labeled material was validated in an endoribonuclease activity assay.

Detalles Bibliográficos
Autores principales: Skipsey, Mark, Hack, Gordon, Hooper, Thomas A., Shankey, Mark C., Conway, Louis P., Schröder, Martin, Hodgson, David R. W.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Taylor & Francis 2013
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3924349/
https://www.ncbi.nlm.nih.gov/pubmed/24328564
http://dx.doi.org/10.1080/15257770.2013.851393
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author Skipsey, Mark
Hack, Gordon
Hooper, Thomas A.
Shankey, Mark C.
Conway, Louis P.
Schröder, Martin
Hodgson, David R. W.
author_facet Skipsey, Mark
Hack, Gordon
Hooper, Thomas A.
Shankey, Mark C.
Conway, Louis P.
Schröder, Martin
Hodgson, David R. W.
author_sort Skipsey, Mark
collection PubMed
description 5′-deoxy-5′-hydrazinylguanosine was incorporated into the 5′-termini of RNA transcripts using T7 RNA polymerase. Transcriptions provided 5′-hydrazinyl-RNA that was readily labeled and purified. The use of fluorophore-labeled material was validated in an endoribonuclease activity assay.
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spelling pubmed-39243492014-02-14 5′-Deoxy-5′-Hydrazinylguanosine as an Initiator of T7 RNA Polymerase-Catalyzed Transcriptions for the Preparation of Labeling-Ready RNAS Skipsey, Mark Hack, Gordon Hooper, Thomas A. Shankey, Mark C. Conway, Louis P. Schröder, Martin Hodgson, David R. W. Nucleosides Nucleotides Nucleic Acids Research Article 5′-deoxy-5′-hydrazinylguanosine was incorporated into the 5′-termini of RNA transcripts using T7 RNA polymerase. Transcriptions provided 5′-hydrazinyl-RNA that was readily labeled and purified. The use of fluorophore-labeled material was validated in an endoribonuclease activity assay. Taylor & Francis 2013-12-11 2013-11 /pmc/articles/PMC3924349/ /pubmed/24328564 http://dx.doi.org/10.1080/15257770.2013.851393 Text en Copyright © Taylor and Francis Group, LLC http://www.informaworld.com/mpp/uploads/iopenaccess_tcs.pdf This is an open access article distributed under the Supplemental Terms and Conditions for iOpenAccess articles published in Taylor & Francis journals (http://www.informaworld.com/mpp/uploads/iopenaccess_tcs.pdf) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Skipsey, Mark
Hack, Gordon
Hooper, Thomas A.
Shankey, Mark C.
Conway, Louis P.
Schröder, Martin
Hodgson, David R. W.
5′-Deoxy-5′-Hydrazinylguanosine as an Initiator of T7 RNA Polymerase-Catalyzed Transcriptions for the Preparation of Labeling-Ready RNAS
title 5′-Deoxy-5′-Hydrazinylguanosine as an Initiator of T7 RNA Polymerase-Catalyzed Transcriptions for the Preparation of Labeling-Ready RNAS
title_full 5′-Deoxy-5′-Hydrazinylguanosine as an Initiator of T7 RNA Polymerase-Catalyzed Transcriptions for the Preparation of Labeling-Ready RNAS
title_fullStr 5′-Deoxy-5′-Hydrazinylguanosine as an Initiator of T7 RNA Polymerase-Catalyzed Transcriptions for the Preparation of Labeling-Ready RNAS
title_full_unstemmed 5′-Deoxy-5′-Hydrazinylguanosine as an Initiator of T7 RNA Polymerase-Catalyzed Transcriptions for the Preparation of Labeling-Ready RNAS
title_short 5′-Deoxy-5′-Hydrazinylguanosine as an Initiator of T7 RNA Polymerase-Catalyzed Transcriptions for the Preparation of Labeling-Ready RNAS
title_sort 5′-deoxy-5′-hydrazinylguanosine as an initiator of t7 rna polymerase-catalyzed transcriptions for the preparation of labeling-ready rnas
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3924349/
https://www.ncbi.nlm.nih.gov/pubmed/24328564
http://dx.doi.org/10.1080/15257770.2013.851393
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